Transplantation of Cultured Neural Cells from Human Fetuses into the Brain of Rats Exposed to Acute Hypoxia

Александрова, М. А.; Revishchin, A. V.; Podgornyi, O. V.; Полтавцева, Р. А.; Marei, M. V.; Korochkin, L. I.; Сухих, Г. Т.

doi:10.1023/b:bebm.0000031565.35782.31

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…The cells cultured by the short and long protocols were injected (as a cell suspension) into the hippocampus of intact rats and animals exposed to hypoxia [1,3,7]. The results of microscopic examination 10, 20, and 30 days after transplantation showed HNSC in the brain of all recipients.…”

Section: Resultsmentioning

confidence: 99%

“…Precultured stem/progenitor cells from human fetal brain were transplanted into intact or damaged brain (hypoxic hypoxia), or into mechanically damaged spinal cord of adult rats [1][2][3][4][5]7].…”

Section: Methodsmentioning

confidence: 99%

“…The cells cultured by both protocols were transplanted into the brain of intact rats and rats exposed to hypoxia [1,3]. Experiments were carried out on adult female Wistar rats.…”

Section: Methodsmentioning

confidence: 99%

“…Some sections were stained by the methods of Niessl, Giemsa, with hematoxylin and eosin, and other sections were studied by immunocytochemical methods using anti Human nuclei (Chemicon; 1:30) and anti-Neu N antibodies to the nuclear protein of differentiated neurons (Chemicon; 1:30) as first antibodies. The sections were processed as described previously [1][2][3][4][5]7].…”

Section: Methodsmentioning

confidence: 99%

“…We studied the effect of the status of recipient brain and the regimen of culturing of the transplanted material on the behavior of human fetal cells transplanted into rat brain [1][2][3][4][5]7,12].…”

mentioning

confidence: 99%

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Human Fetal Neural Stem Cells in Rat Brain: Effects of Preculturing and Transplantation

et al. 2005

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The fate of human fetal stem/progenitor cells transplanted into rat brain depends on conditions of preculturing (long or short) and state and site of transplantation. Human nestin-positive stem cells cultured according to the short protocol did not migrate into hypoxic and normal brain after transplantation, but actively migrated in damaged spinal cord. After transplantation of long-cultured cells into the brain mainly committed neuroblasts and solitary nestin-positive cells migrated from the site of transplantation into the brain.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The cells cultured by both protocols were transplanted into the brain of intact rats and rats exposed to hypoxia [1,3]. Experiments were carried out on adult female Wistar rats.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Human Fetal Neural Stem Cells in Rat Brain: Effects of Preculturing and Transplantation

et al. 2005

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Differentiation of Dissociated Rat Embryonic Brain after Allotransplantation into Damaged Nerve

2013

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Fragments of the dorsolateral wall of the anterior brain vesicle from rat embryos on embryonic day 15 were dissociated and the resultant suspension containing single cells and cell aggregates was injected into the proximal segment of crushed sciatic nerve of adult animals for evaluation of their engrafting and differentiation under conditions of changed microenvironment. On days 1 and 21 postoperation, Msi-1, GFAP, NeuN, vimentin, and PCNA were detected by immunohistochemical methods. Small clusters of Msi-1-immunopositive cells were detected in the nerve trunks on the next day after transplantation. On day 21 after surgery, these precursors differentiate into nerve cells, astrocytes, and primarily ependymocytes.

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Differentiation of Cholinergic Neurons in Rat Spinal Cord Under Conditions of Allotransplantation into a Peripheral Nerve and In Situ Development

2015

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The method of ectopic transplantation of embryonic anlages of CNS allows studying histoblastic potencies of progenitor cells developing under conditions of changed microenvironment. Some progenitor cells in the transplants of rat embryonic spinal cord retained their ability to express choline acetyltransferase after transplantation into the sciatic nerve of adult animals. Comparative analysis of cholinergic neurons in the neurotransplants and neurons formed in rat spinal cord during normal ontogeny showed that choline acetyltransferase-positive cells after transplantation into the nerve reached morphological differentiation of motor neurons at later terms than cells developing in situ. They were scattered one by one and did not form nuclear nerve centers. We did not fi nd structures similar to presynaptic cholinergic buds typical of intact spinal cord near these cells throughout the observation period. Solitary cholinergic neurons survived in the transplants for 19 months.

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Transplantation of Cultured Neural Cells from Human Fetuses into the Brain of Rats Exposed to Acute Hypoxia

Cited by 6 publications

References 7 publications

Human Fetal Neural Stem Cells in Rat Brain: Effects of Preculturing and Transplantation

Human Fetal Neural Stem Cells in Rat Brain: Effects of Preculturing and Transplantation

Differentiation of Dissociated Rat Embryonic Brain after Allotransplantation into Damaged Nerve

Differentiation of Cholinergic Neurons in Rat Spinal Cord Under Conditions of Allotransplantation into a Peripheral Nerve and In Situ Development

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