Differentiation of Dissociated Rat Embryonic Brain after Allotransplantation into Damaged Nerve

Abstract

Fragments of the dorsolateral wall of the anterior brain vesicle from rat embryos on embryonic day 15 were dissociated and the resultant suspension containing single cells and cell aggregates was injected into the proximal segment of crushed sciatic nerve of adult animals for evaluation of their engrafting and differentiation under conditions of changed microenvironment. On days 1 and 21 postoperation, Msi-1, GFAP, NeuN, vimentin, and PCNA were detected by immunohistochemical methods. Small clusters of Msi-1-immunopositive cells were detected in the nerve trunks on the next day after transplantation. On day 21 after surgery, these precursors differentiate into nerve cells, astrocytes, and primarily ependymocytes.

Effect of Allotransplants Containing Dissociated Cells of Rat Embryonic Spinal Cord on Nerve Fiber Regeneration in a Recipient

“…The cell suspensions containing ≥85% viable cells were used for transplantation. The cell suspension (3-7×10 4 ) in 5 μl medium was injected under nerve trunk perineurium of the previously damaged sciatic nerve of adult animals [6]. The control group received no cell transplantation.…”

Stem Cell Technology in Peripheral Nerve Restoration

“…Первиною метою цих експериментів було намагання довести принципову можливість такого виду трансплантації та дослідження подальшого диференціювання клітин ембріональної нервової тканини і їх вплив на регенерацію ушкодженого нерва [79]. Гарні результати були отримані при алогенній трансплантації ембріональної нервової тканини в експерименті, що підтверджено даними електрофізіологічного та морфологічного досліджень [80,81].…”