Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones

Kitoh, Hiroshi; Kitakoji, Takahiko; Tsuchiya, Hiroki; Katoh, Masahiko; Ishiguro, Naoki

doi:10.1016/j.bone.2006.09.019

Cited by 114 publications

(67 citation statements)

References 29 publications

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“…Furthermore, the cells implanted for bone reconstruction is usually ten to one hundred million (35)(36) . Based on previous ASC expansions we supposed to be able to obtain 6 x 10 8 cells by passage 7.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Bellotti

Stanco

Ragazzini

et al. 2013

Int J Immunopathol Pharmacol

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Mesenchymal stem cells (MSC) and adipose-derived stem cells (ASC) were recently proposed for bone maxillofacial reconstruction in association with biomaterials. For this application MSC must be ex-vivo expanded in order to obtain, for a given volume of implanted biomaterial, a relevant number of bone forming cells. Previously conducted pre-clinical studies suggested that a concentration of 6 x 10 8 ASC associated with 900 mg of anorganic bovine bone (ABB) could be effective for human maxillary sinus floor elevation. A keystone issue to guarantee the quality and safety of Advanced Therapy Medicinal Products containing expanded MSC and ASC is their chromosome stability in culture: this topic has been widely investigated and conflicting results have been published. Abnormal karyotype of human ex-vivo expanded MSC and ASC was found by some authors, while, at the same time, several other studies showed the MSC and ASC karyotype to be normal. It is therefore important that all the results obtained on MSC andASC karyotype analysis be published. Given this context, the aim of this manuscript, aim of this manuscript is to verify the karyotype stability of ASC in view oftheir applications in clinical trials. ASC obtained from the adipose tissue of 4 donors were expanded over extended culture time. Based on previous ASC expansions we hypothesized to be able to obtain 6 x lOS cells by passage 7. Karyotype analysis 000 metaphases was planned to be investigated at passage 2, 7, and 15 in all the cultures. No abnormalities were found in the karyotype of two donors at all the passages tested, while a translocation was found in 2 metaphases of a donor at passage 7, but not at passage 15, and in the fourth donor in 5 metaphases a trisomy was found at passage 15. Chromosomal abnormalities were detected only after extended ASC expansion. Whether these anomalies can be related to risk for the patient's safety will have to be demonstrated by in-vivo studies.

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Section: Discussionmentioning

confidence: 99%

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Bellotti

Stanco

Ragazzini

et al. 2013

Int J Immunopathol Pharmacol

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“…The use of platelet rich plasma alone was reported to give positive results in a human study (Latalski et al, 2011). The positive osteogenic effect of culture expanded bone marrow cells has also been reported when combined with platelet-rich plasma in humans and with bFGF in rabbits (Jiang et al, 2010;Kitoh et al, 2007).…”

Section: Cell Therapy and Platelet-rich Plasmamentioning

confidence: 95%

Distraction Osteogenesis and Its Challenges in Bone Regeneration

Hamdy¹,

Rendon²,

Tabrizian³

2012

Bone Regeneration

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“…In 2007, Richards et al [47] observed marrow-derived progenitor cell injections enhanced new bone formation in a femoral distraction model of rat. In 2007, Kitoh et al [30,31] showed transplantation of culture-expanded bone marrow cells combined with PRP accelerated new bone formation during the distraction osteogenesis, especially in the femur, but with many disadvantages [47]. As for PRP, limited reports could be found [13,28,30,33].…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Aspirate Concentrate and Platelet-rich Plasma Enhanced Bone Healing in Distraction Osteogenesis of the Tibia

Lee¹,

Ryu²,

Kim³

et al. 2014

Clin Orthop Relat Res

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Background During lower limb lengthening, poor bone regeneration is a devastating complication. Several local or systemic applications have been used to promote osteogenesis, and biologic stimulations are gaining attention, but their utility has not been proven in this setting. Questions/purposes In patients undergoing bilateral tibial lengthening, we compared those receiving an osteotomy site injection of autologous bone marrow aspirate concentrate (BMAC) plus platelet-rich plasma (PRP) with those not receiving such an injection in terms of external fixator index (time in external fixation divided by amount of lengthening), full weightbearing index (time until a patient was permitted to do full weightbearing divided by amount of lengthening), four cortical healing indexes (time until each cortical union divided by amount of lengthening), and callus shape and type. Methods Twenty-two patients (44 tibias) undergoing bilateral tibial lengthening enrolled in this randomized trial. Two patients were excluded, one due to insufficient radiographic evaluation and one who was lost to followup, leaving 20 patients (40 segments) for inclusion. Ten patients (20 segments) received BMAC combined with PRP injection (treatment group) and 10 patients (20 segments) received no injection (control group). All patients underwent stature lengthening for familial short stature with the lengthening over nail technique. Autologous BMAC combined with PRP was injected at the tibial osteotomy site at the end of the index surgery. Mean distraction rates were similar between groups (0.75 mm/day in the treatment group versus 0.72 mm/day in the control group; p = 0.24). Full weightbearing was permitted when we observed radiographic evidence of healing at two cortices; this assessment was made by the surgeon who was blinded to the treatment each patient received. Minimum followup was 24 months (mean, 28 months; range, 24-34 months).

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Transplantation of culture expanded bone marrow cells and platelet rich plasma in distraction osteogenesis of the long bones

Cited by 114 publications

References 29 publications

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Distraction Osteogenesis and Its Challenges in Bone Regeneration

Bone Marrow Aspirate Concentrate and Platelet-rich Plasma Enhanced Bone Healing in Distraction Osteogenesis of the Tibia

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