Transplantation of cryopreserved cardiomyocytes

Abstract

The present study uncovers the following key observations: (1) cryopreservation of fetal cardiomyocytes and cardiomyocytes isolated from cryopreserved myocardial tissue results in viable and functional cells, (2) cryopreserved fetal cardiomyocytes can be successfully transplanted into subcutaneous and myocardial scar tissue, and (3) improvements in cryopreservation techniques are required to augment the rates of cardiomyocyte survival observed in the study.

Perkutane intramyokardiale Implantation von autologen Myoblasten bei ischämischer Kardiomyopathie

“…Als ein experimenteller therapeutischer Ansatz zur Behandlung der ischämischen Kardiomyopathie rückt die Zelltransplantation zunehmend in das klinische Interesse [9,20]. Mit dem Ziel, nekrotisches Gewebe durch funktionsfähige Zellen zu ersetzen, haben verschiedene Zelltypen in tierexperimentellen Modellen Hoffnungen geweckt, insbesondere die Verwendung von embryonalen Stammzellen [18], fetalen Kardiomyozyten [43], autologen Myoblasten [29], hämatopoetischen Stammzellen [26], mesenchymalen Stammzellen [39] und endothelialen Progenitorzellen [16]. Histologisch konnten Regeneration von kardialen Strukturen und vermehrte Angiogenese nachgewiesen werden, welche mit einer messbaren funktionellen Verbesserung des Herzens einhergehen.…”

Transcatheter Transplantation of Autologous Skeletal Myoblasts in Postinfarction Patients With Severe Left Ventricular Dysfunction

“…Cell transplantation is emerging as a potential novel therapeutic approach for endstage cardiac disease. 1,2 With the aim of replacing necrotic dysfunctional tissue, cells from different sources have been implanted in animal models of myocardial infarction, including embryonic stem cells, 3 fetal cardiomyocytes, 4 skeletal myoblasts, 5 hematopoietic stem cells, 6 mesenchymal stem cells, 7 and endothelial progenitor cells. 8 Most studies have shown some engraftment of donor cells and reconstitution of cardiac structures, i.e., cardiomyocytes and blood vessels, in association with improved heart function.…”

Preservation of rat skeletal muscle function during storage for 16 h at 4 °C is not improved by pre-storage perfusion

“…Other methods of cooling, such as cryopreservation, appear to be a promising possibility in the future.To date, however, this preservation technique has been successful for isolated cells [38] and tissue slices of a few cell layers in thickness [39,40], but not for intact organs such as muscles [41].…”