Preservation of rat skeletal muscle function during storage for 16 h at 4 °C is not improved by pre-storage perfusion

Heijden, E.P.A. Brigitte van der; Kroese, A.B.A.; Werker, Paul M. N.; Kon, Moshe; Bär, Dop

doi:10.1042/cs20020308

Cited by 4 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a 2003 study, Van Der Heiden et al compared six perfused and six non-perfused muscle tissues to determine whether perfusion before storage at +4°C for 16 hours improves the properties of muscle tissues. [ 14 ] UW and HTK solutions were used for perfusion with both groups of muscle tissues incubated at +4°C for 16 hours. Comparison was conducted by measuring the function of the muscle tissue, and it was shown that perfusion did not yield any improvement of muscle tissue function.…”

Section: Discussionmentioning

confidence: 99%

Preservative solution for skeletal muscle biopsy samples

Kurt

Özcan

et al. 2015

Ann Indian Acad Neurol

View full text Add to dashboard Cite

Context:Muscle biopsy samples must be frozen with liquid nitrogen immediately after excision and maintained at -80°C until analysis. Because of this requirement for tissue processing, patients with neuromuscular diseases often have to travel to centers with on-site muscle pathology laboratories for muscle biopsy sample excision to ensure that samples are properly preserved.Aim:Here, we developed a preservative solution and examined its protectiveness on striated muscle tissues for a minimum of the length of time that would be required to reach a specific muscle pathology laboratory.Materials and Methods:A preservative solution called Kurt-Ozcan (KO) solution was prepared. Eight healthy Sprague-Dawley rats were sacrificed; striated muscle tissue samples were collected and divided into six different groups. Muscle tissue samples were separated into groups for morphological, enzyme histochemical, molecular, and biochemical analysis.Statistical method used:Chi-square and Kruskal Wallis tests.Results:Samples kept in the KO and University of Wisconsin (UW) solutions exhibited very good morphological scores at 3, 6, and 18 hours, but artificial changes were observed at 24 hours. Similar findings were observed for the evaluated enzyme activities. There were no differences between the control group and the samples kept in the KO or UW solution at 3, 6, and 18 hours for morphological, enzyme histochemical, and biochemical features. The messenger ribonucleic acid (mRNA) of β-actin gene was protected up to 6 hours in the KO and UW solutions.Conclusion:The KO solution protects the morphological, enzyme histochemical, and biochemical features of striated muscle tissue of healthy rats for 18 hours and preserves the mRNA for 6 hours.

show abstract

Section: Discussionmentioning

confidence: 99%

Preservative solution for skeletal muscle biopsy samples

Kurt

Özcan

et al. 2015

Ann Indian Acad Neurol

View full text Add to dashboard Cite

show abstract

“…Flush-out with HTK (for composition, see [12]) at 4 • C was performed at a rate of 40 µl/min during 10 min [19] under continuous monitoring of perfusion pressure through the femoral artery catheter to ensure that high pressures in the cremaster muscle were avoided. Flush-out was not only performed to remove all blood cells from the cremaster muscle, but also to ensure that the entire vascular bed was filled with HTK during the ischaemic period.…”

Section: Methodsmentioning

confidence: 99%

Effect of hypothermia and HTK on the microcirculation in the rat cremaster muscle after ischaemia

et al. 2005

Self Cite

View full text Add to dashboard Cite

Hypothermia is an important preservation method for tissues and solid organs. The aim of the present study was to assess in rat cremaster muscle the effect of hypothermia, without or with pre-ischaemic HTK (histidine-tryptophan-ketoglutarate-Bretschneider solution) perfusion, on microvascular consequences of 4 or 6 h ischaemia and 2 h of reperfusion. Intravital microscopy was applied to examine capillary perfusion and leucocyte-endothelium interactions. The cremaster muscle was subjected to 4 or 6 h of cold (4 degrees C) or warm (33-34 degrees C) ischaemia and 2 h of reperfusion. Measurements were performed at baseline, prior to HTK perfusion and ischaemia, and at 0, 1 and 2 h after blood flow restoration. Hypothermia completely prevented the 50% reduction in capillary perfusion that was observed previously at start of reperfusion after 4 h warm ischaemia. After 6 h of warm ischaemia, perfusion resumed in only 45% of capillaries and remained at this low level during reperfusion. In contrast, only a slight decrease (< 10%) in capillary perfusion was observed after 6 h of cold ischaemia. Pre-ischaemic HTK perfusion had no beneficial effect on tissue perfusion. Both hypothermia and HTK attenuated the significant increase in venular leucocyte-vessel wall interactions, which was observed after 4 h of warm ischaemia in a previous study. Combined application of both interventions had no additional effects. After 6 h of warm ischaemia, no increase in leucocyte-vessel wall interactions was observed, possibly due to venular flow reduction. In conclusion, hypothermia preserves capillary perfusion and prevents an increase in leucocyte-vessel wall interactions during reperfusion after muscle tissue ischaemia. Preischaemic perfusion of the vasculature with HTK does not improve the effects of cold storage on tissue perfusion, but attenuates the inflammatory response independently of temperature effect.

show abstract

“…Therefore, in 8 of 26 preparations the vascular territory supplied by the feed arteries of the RET was investigated by intravascular injection with plastic as described [19,20] . Briefly, 12 parts of Araldite F with 7.5% Microlith-T pigment was mixed with 4 parts of diluent DY 026 SP and 9 parts of hardener HY 2967 (all from Ciba-Geigy BV, Arnhem, The Netherlands).…”

Section: Vascular Territorymentioning

confidence: 99%

Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

With

Vries

Kroese³

et al. 2008

Eur Surg Res

View full text Add to dashboard Cite

Background: The hamster retractor muscle (RET) is used as an in vivo model in studies of skeletal muscle ischemia-reperfusion injury. The RET is unique in that the muscle can be isolated while preserving the primary vascular supply so that its contractile function can be measured simultaneously with local microvascular responses to experimental interventions. The goal of this study was to understand the anatomical origin of the vascular supply to the RET and determine whether the RET can be used as a free flap after surgical isolation of the thoracodorsal vessels. Methods: Microdissection was performed to determine the anatomy of the vasculature that supplies and drains the RET. Results: Distinct numbers and patterns of feed arteries (2–4) and collecting veins (1–3) were identified (n = 26 animals). Dye injection (n = 8) of the thoracodorsal artery demonstrated that the RET remains perfused following its isolation on the thoracodorsal pedicle. Heterotopic allograft transplantation of the RET (n = 2) was performed by anastomosing the thoracodorsal vessels to the femoral vessels using the end-to-side technique. Conclusions: The anatomical relationships indicate that the RET can be used as a free flap model for evaluating the effect of preservation strategies and transplantation on skeletal muscle microcirculation and contractile function.

show abstract

Preservation of rat skeletal muscle function during storage for 16 h at 4 °C is not improved by pre-storage perfusion

Cited by 4 publications

References 33 publications

Preservative solution for skeletal muscle biopsy samples

Preservative solution for skeletal muscle biopsy samples

Effect of hypothermia and HTK on the microcirculation in the rat cremaster muscle after ischaemia

Vascular Anatomy of the Hamster Retractor Muscle with Regard to Its Microvascular Transfer

Contact Info

Product

Resources

About