Transplantation of BMSCs expressing hPDGF-A/hBD2 promotes wound healing in rats with combined radiation-wound injury

Liu, Hao; Wang, J; Zou, Zhongmin; Gao, Yan; Dong, Shiwu; Deng, Jun; Ran, Xinze; Feng, Yimei; Luo, Chao; Wang, Y; Cheng, Tianmin

doi:10.1038/gt.2008.133

Cited by 59 publications

(46 citation statements)

References 29 publications

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“…28,29 In conclusion, we prepared an ADM from nude mice and demonstrated that the ASC-seeded ADM improved wound healing. Moreover, cell-tracking experiments showed that the ASCs contributed to the newly formed vasculature and that VEGF-expressing ASCs could be detected after transplantation.…”

mentioning

confidence: 84%

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Huang

Hsu

Chang

et al. 2012

Annals of Plastic Surgery

117

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mentioning

confidence: 84%

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Huang

Hsu

Chang

et al. 2012

Annals of Plastic Surgery

117

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“…77 Hao et al examined the role of the BMSCs in combination with gene therapy. 78 BMSCs isolated from rats were transfected by an adenovirus with 2 human genes, the PDGF and beta-defensin2 (BD2). More granulation tissue, collagen deposition, and less bacterial colonization in the wounds treated with the transfected stem cells were observed.…”

Section: Stem Cells In Wound Healingmentioning

confidence: 99%

Adipose-Derived Stem Cells for Wound Healing Applications

Cherubino¹,

Rubin²,

Miljković³

et al. 2011

Annals of Plastic Surgery

149

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Nonhealing wounds remain a significant challenge for plastic surgeons. More than 600,000 people suffer from venous ulcers and 1.5 to 3 million people are being treated for pressure sores every year in the United States. The use of tissue engineering techniques such as stem-cell therapy and gene therapy to improve wound healing is a promising strategy. Adipose tissue represents a source of cells that may be able to enhance wound healing. Adipose-derived stem cells (ASCs) are adult stem cells that are easily harvested and of great interest for plastic surgeons. Specifically, ASCs secrete angiogenic growth factors that can induce tissue regeneration. This review describes innovative research strategies using ASCs therapies for treatment of chronic, nonhealing wounds.

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“…Most current methods involve viral vectors to achieve gene delivery in order to overexpress therapeutic factors in situ. 8,9 Although these approaches produce impressive results, 4,10 their promise for broad clinical application is limited due to risks of immunogenicity, insertional mutagenesis, and tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived Stromal Cells Overexpressing Vascular Endothelial Growth Factor Accelerate Mouse Excisional Wound Healing

et al. 2013

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Angiogenesis is essential to wound repair, and vascular endothelial growth factor (VEGF) is a potent factor to stimulate angiogenesis. Here, we examine the potential of VEGF-overexpressing adipose-derived stromal cells (ASCs) for accelerating wound healing using nonviral, biodegradable polymeric vectors. Mouse ASCs were transfected with DNA plasmid encoding VEGF or green fluorescent protein (GFP) using biodegradable poly (β-amino) esters (PBAE). Cells transfected using Lipofectamine 2000, a commercially available transfection reagent, were included as controls. ASCs transfected using PBAEs showed enhanced transfection efficiency and 12-15-fold higher VEGF production compared with cells transfected using Lipofectamine 2000 (*P < 0.05). When transplanted into a mouse wild-type excisional wound model, VEGF-overexpressing ASCs led to significantly accelerated wound healing, with full wound closure observed at 8 days compared to 10-12 days in groups treated with ASCs alone or saline control (*P < 0.05). Histology and polarized microscopy showed increased collagen deposition and more mature collagen fibers in the dermis of wound beds treated using PBAE/VEGF-modified ASCs than ASCs alone. Our results demonstrate the efficacy of using nonviral-engineered ASCs to accelerate wound healing, which may provide an alternative therapy for treating many diseases in which wound healing is impaired.

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Transplantation of BMSCs expressing hPDGF-A/hBD2 promotes wound healing in rats with combined radiation-wound injury

Cited by 59 publications

References 29 publications

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Adipose-Derived Stem Cells for Wound Healing Applications

Adipose-derived Stromal Cells Overexpressing Vascular Endothelial Growth Factor Accelerate Mouse Excisional Wound Healing

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