Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Huang, Shengping; Hsu, Chia-Hao; Chang, Stephen S.; Wang, Chih-Hsin; Deng, Shou-Cheng; Dai, Niann‐Tzyy; Chen, Tim-Mo; Chan, James; Chen, Shyi-Gen; Huang, Shih‐Ming

doi:10.1097/sap.0b013e318273f909

Cited by 120 publications

(94 citation statements)

References 28 publications

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“…Aliquots of MSCs were characterized by their adhesiveness and fusiform shape. They were also characterized by flow cytometry for the surface markers CD45− and CD29+ and by differentiation [28].…”

Section: Isolation and Culture Of At-mscsmentioning

confidence: 99%

Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations

et al. 2014

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Vascular endothelial dysfunction plays a key role in the pathogenesis of Alzheimer's disease (AD). Patients with AD have displayed decreased circulating endothelial progenitor cells (EPCs) which repair and maintain the endothelial function. Transplantation of EPCs has emerged as a promising approach for the management of cerebrovascular diseases including ischemic stroke, however, its impact on AD has been poorly described. Thus, the current study aimed at investigating the effects of bone marrow-derived (BM) EPCs transplantation in repeated scopolamine-induced cognitive impairment, an experimental model that replicates biomarkers of AD. Intravenously transplanted BM-EPCs migrated into the brain of rats and improved the learning and memory deficits. Meanwhile, they mitigated the deposition of amyloid plaques and associated histopathological alterations. At the molecular levels, BM-EPCs blunted the increase of hippocampal amyloid beta protein (Aβ), amyloid precursor protein (APP) and reinstated the Aβ-degrading neprilysin together with downregulation of p-tau and its upstream glycogen synthase kinase-3β (GSK-3β). They also corrected the perturbations of neurotransmitter levels including restoration of acetylcholine and associated esterase along with dopamine, GABA, and the neuroexitatory glutamate. Furthermore, BM-EPCs induced behavioral recovery via boosting of vascular endothelial growth factor (VEGF), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and its upstream cAMP response element binding (CREB), suppression of the proinflammatory tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and upregulation of interleukin-10 (IL-10). BM-EPCs also augmented Nrf2 and seladin-1. Generally, these actions were analogous to those exerted by adipose tissue-derived mesenchymal stem cells (AT-MSCs) and the reference anti-Alzheimer donepezil. For the first time, these findings highlight the beneficial actions of BM-EPCs against the memory deficits and AD-like pathological dysfunction.

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Section: Isolation and Culture Of At-mscsmentioning

confidence: 99%

Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations

et al. 2014

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“…116,123 In one animal study, the use of ASCs seeded on an acellular dermal matrix found that it enhanced wound healing, promoted angiogenesis, and contributed to newly formed vasculature in murine mouse models. 124 Finally, MSC treatment of wounds reduces inflammation, accelerates wound healing, promotes granulation tissue, and increases angiogenesis. 2 MSC safety has been evaluated in a present metaanalysis and at this time appears to be safe for clinical use.…”

Section: Mscs: Mechanism(s) Of Actionmentioning

confidence: 99%

Mesenchymal Stem Cells in Chronic Wounds: The Spectrum from Basic to Advanced Therapy

Otero-Viñas

Falanga

2016

Advances in Wound Care

121

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“…Successfully using MSCs for skin regeneration requires a sufficient quantity of MSCs and effective delivery of the MSCs to the wound site. Theoretically, ADM can serve as a scaffold for the delivery of MSCs to the target wound, while the MSCs promote retention and neovascularization of the scaffold [27].…”

Section: Discussionmentioning

confidence: 99%

Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing

et al. 2015

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Adipose-Derived Stem Cells Seeded on Acellular Dermal Matrix Grafts Enhance Wound Healing in a Murine Model of a Full-Thickness Defect

Cited by 120 publications

References 28 publications

Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations

Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations

Mesenchymal Stem Cells in Chronic Wounds: The Spectrum from Basic to Advanced Therapy

Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing

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