Transplantation of autologous endothelial progenitor cells in porous PLGA scaffolds create a microenvironment for the regeneration of hyaline cartilage in rabbits

Chang, Nai-Jen; Lam, Chen Fuh; Lin, Chih‐Chan; Chen, W.-L.; Li, C.-F.; Lin, Yi Ting; Yeh, Ming Long

doi:10.1016/j.joca.2013.07.016

Cited by 46 publications

(36 citation statements)

References 36 publications

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“…After 12 weeks after implantation, the EPC-PLGA was the only group that demonstrated the development of new cartilaginous tissue with a transparent, smooth and host integrated articular surface. [189] Still, compared to the other groups, the EPC-PLGA one was associated with higher SOX9 expression, and greater glycosaminoglycan and collagen type II content, the major collagen type of cartilage. Moreover, it was verified an arranged osteochondral integration and the development of vessel-rich tubercular bone.…”

Section: Docetaxelmentioning

confidence: 75%

“…Chang et al investigated a cell-based approach by seeding autologous endothelial progenitor cells (EPCs) into the PLGA scaffold to repair a full-thickness osteochondral defect in rabbits. [189] The animals were randomly divided into three groups: the empty defect group, the PLGA group, and the EPC-PLGA group. After 12 weeks after implantation, the EPC-PLGA was the only group that demonstrated the development of new cartilaginous tissue with a transparent, smooth and host integrated articular surface.…”

Section: Docetaxelmentioning

confidence: 99%

“…Moreover, it was verified an arranged osteochondral integration and the development of vessel-rich tubercular bone. [189] Ren et al studied the reconstructive functions of mesenchymal stem cells (MSCs) in PLGA scaffolds in mandibular defect of rabbit using as a control group PLGA scaffold alone and a blank group without scaffold. [190] The results showed that the PLGAMSCs could greatly promote cell growth with the defect completely recovered after 3 months, while in the other groups the defects could not be repaired.…”

Section: Docetaxelmentioning

confidence: 99%

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Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Martins

Sousa

Araújo

et al. 2017

Adv Healthcare Materials

195

133

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Poly(lactic‐co‐glycolic) acid (PLGA) is one of the most versatile biomedical polymers, already approved by regulatory authorities to be used in human research and clinics. Due to its valuable characteristics, PLGA can be tailored to acquire desirable features for control bioactive payload or scaffold matrix. Moreover, its chemical modification with other polymers or bioconjugation with molecules may render PLGA with functional properties that make it the Holy Grail among the synthetic polymers to be applied in the biomedical field. In this review, the physical–chemical properties of PLGA, its synthesis, degradation, and conjugation with other polymers or molecules are revised in detail, as well as its applications in drug delivery and regeneration fields. A particular focus is given to successful examples of products already on the market or at the late stages of trials, reinforcing the potential of this polymer in the biomedical field.

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Section: Docetaxelmentioning

confidence: 75%

Section: Docetaxelmentioning

confidence: 99%

Section: Docetaxelmentioning

confidence: 99%

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Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Martins

Sousa

Araújo

et al. 2017

Adv Healthcare Materials

195

133

View full text Add to dashboard Cite

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“…For implantation purposes in vivo, BMSC seeding densities of 1-50 × 10 6 cells/cm 3 of biomaterial matrix have been used in preclinical animal studies. 1,14,[23][24][25][26][27][28][29][30][31][32] In clinical studies, a density of 5 × 10 6 cells/cm 3 has been reported although the rationale for adopting this seeding density was not described. 3,4 The objective of this study was to assess the impact of cell seeding density within a clinically relevant, collagen I scaffold on in vitro BMSC chondrogenesis following 2D and 3D isolation and expansion.…”

Section: Introductionmentioning

confidence: 99%

Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold

Bornes

Jomha

Mulet‐Sierra

et al. 2016

Tissue Engineering Part C: Methods

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Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects. The objective of this study was to assess the impact of cell seeding density within a collagen I scaffold on in vitro BMSC chondrogenesis following isolation and expansion in two-dimensional (2D) and three-dimensional (3D) environments. It was hypothesized that both expansion protocols would produce BMSCs capable of hyaline-like chondrogenesis with an optimal seeding density of 10 × 10 6 cells/cm 3 . Ovine BMSCs were isolated in a 2D environment by plastic adherence, expanded to passage two in flasks containing an expansion medium, and seeded within collagen I scaffolds at densities of 50, 10, 5, 1, and 0.5 × 10 6 BMSCs/cm 3 . For 3D isolation and expansion, aspirates containing known quantities of mononucleated cells (bone marrow-derived mononucleated cells [BMNCs]) were seeded on scaffolds at 50, 10, 5, 1, and 0.5 × 10 6 BMNCs/cm 3 and cultured in the expansion medium for an equivalent duration to 2D expansion. Constructs were differentiated in vitro in the chondrogenic medium for 21 days and assessed with reverse-transcription quantitative polymerase chain reaction, safranin O staining, histological scoring using the Bern Score, collagen immunofluorescence, and glycosaminoglycan (GAG) quantification. Two-dimensional-expanded BMSCs seeded at all densities were capable of proteoglycan production and displayed increased expressions of aggrecan and collagen II messenger RNA (mRNA) relative to pre-differentiation controls. Collagen II deposition was apparent in scaffolds seeded at 0.5-10 × 10 6 BMSCs/cm 3 . Chondrogenesis of 2D-expanded BMSCs was most pronounced in scaffolds seeded at 5-10 × 10 6 BMSCs/cm 3 based on aggrecan and collagen II mRNA, safranin O staining, Bern Score, total GAG, and GAG/deoxyribonucleic acid (DNA). For 3D-expanded BMSC-seeded scaffolds, increased aggrecan and collagen II mRNA expressions relative to controls were noted with all densities. Proteoglycan deposition was present in scaffolds seeded at 0.5-50 × 10 6 BMNCs/cm 3 , while collagen II deposition occurred in scaffolds seeded at 10-50 × 10 6 BMNCs/cm 3 . The

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“…MSCs are historically obtained from bone marrow aspirates (BMSCs) 26 , and more recently from other tissue sources: adipose tissue 27 (adipose-derived stem cells - ADSCs), amniotic fluid 28 (AFSCs), synovium 29, 30 and periosteum 31 . The use of peripheral blood has also been reported clinically, both for obtaining stem cells 32 and progenitor cells 33 . Recently, multipotent adult progenitor cells (MAPC) are gaining more attention, as potentially better candidate seed cells for OC grafts.…”

Section: Biomimetic System Component I: Cellsmentioning

confidence: 99%

Challenges in engineering osteochondral tissue grafts with hierarchical structures

Gadjanski

Vunjak-Novakovic

2015

Expert Opinion on Biological Therapy

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Introduction A major hurdle in treating osteochondral (OC) defects are the different healing abilities of two types of tissues involved - articular cartilage and subchondral bone. Biomimetic approaches to OC-construct-engineering, based on recapitulation of biological principles of tissue development and regeneration, have potential for providing new treatments and advancing fundamental studies of OC tissue repair. Areas covered This review on state of the art in hierarchical OC tissue graft engineering is focused on tissue engineering approaches designed to recapitulate the native milieu of cartilage and bone development. These biomimetic systems are discussed with relevance to bioreactor cultivation of clinically sized, anatomically shaped human cartilage/bone constructs with physiologic stratification and mechanical properties. The utility of engineered OC tissue constructs is evaluated for their use as grafts in regenerative medicine, and as high-fidelity models in biological research. Expert opinion A major challenge in engineering OC tissues is to generate a functionally integrated stratified cartilage-bone structure starting from one single population of mesenchymal cells, while incorporating perfusable vasculature into the bone, and in bone-cartilage interface. To this end, new generations of advanced scaffolds and bioreactors, implementation of mechanical loading regimens, and harnessing of inflammatory responses of the host will likely drive the further progress.

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Transplantation of autologous endothelial progenitor cells in porous PLGA scaffolds create a microenvironment for the regeneration of hyaline cartilage in rabbits

Abstract: The present EPC-PLGA cell delivery system generates a suitable in situ microenvironment for osteochondral regeneration without the supplement of exogenous growth factors.

Cited by 46 publications

References 36 publications

Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Optimal Seeding Densities for In Vitro Chondrogenesis of Two- and Three-Dimensional-Isolated and -Expanded Bone Marrow-Derived Mesenchymal Stromal Stem Cells Within a Porous Collagen Scaffold

Challenges in engineering osteochondral tissue grafts with hierarchical structures

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