Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Martins, Cláudia; Sousa, Flávia; Araújo, Francisca; Sarmento, Bruno

doi:10.1002/adhm.201701035

Cited by 164 publications

(115 citation statements)

References 289 publications

(227 reference statements)

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“…In case of PLGA, a complete degradation of NPs was observed at pH 4.8 within 40 days, whereas at pH 7.4, an 80% degradation was observed after 60 days ( Fig. 2C), which is in line with previous studies [32][33][34]. Considering the degradation profile of the polymers in an acidic medium, the release of BRP-187 from Acdex NPs is expected to be fast due to the rapid degradation of the polymer, whereas the drug release from PLGA NPs is expected to be slower due to the diffusion of drug from the polymer matrix, since degradation of this polymer is very slow [35].…”

Section: Degradation Profile Of the Nanoparticlessupporting

confidence: 92%

Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity

Shkodra

Kretzer

Jordan

et al. 2020

Preprint

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Background: Dual inhibitors of the 5-lipoxygenase-activating protein (FLAP) and the microsomal prostaglandin E2 synthase-1 (mPGES-1) may exert better anti-inflammatory efficacy and lower risks of adverse effects versus non-steroidal anti-inflammatory drugs. Despite these advantages, many dual FLAP/mPGES-1 inhibitors are acidic lipophilic molecules with low solubility and strong tendency for plasma protein binding that limit their bioavailability and bioactivity. Here, we present the encapsulation of the dual FLAP/mPGES-1 inhibitor BRP-187 into the biocompatible polymers acetalated dextran (Acdex) and poly(lactic-co-glycolic acid) (PLGA) via nanoprecipitation.Results: The nanoparticles containing BRP-187 were prepared by the nanoprecipitation method and analyzed by dynamic light scattering regarding their hydrodynamic diameter, by scanning electron microscopy for morphology properties, and by UV-VIS spectroscopy for determination of the encapsulation efficiency of the drug. Moreover, we designed fluorescent BRP-187 particles, which showed high cellular uptake by leukocytes, as analyzed by flow cytometry. Finally, BRP-187 nanoparticles were tested in human polymorphonuclear leukocytes and macrophages to determine drug uptake, cytotoxicity, and efficiency to inhibit FLAP and mPGES-1.Conclusion: Our results demonstrate that encapsulation of BRP-187 into Acdex and PLGA is feasible, and both PLGA-and Acdex-based particles loaded with BRP-187 are more efficient in suppressing 5-lipooxygenase product formation and prostaglandin E2 biosynthesis in intact cells as compared to the free compound, particularly after prolonged preincubation periods.

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Section: Degradation Profile Of the Nanoparticlessupporting

confidence: 92%

Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity

Shkodra

Kretzer

Jordan

et al. 2020

Preprint

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“…5,37-39 Therefore, it is likely that a fibrous membrane system that generates a fiber architecture and mineral composition will increase the feasibility of tendon to bone healing and may be the key to improve clinical prognosis. PLGA is considered an excellent biomaterial for bone tissue engineering due to its satisfactory biocompatibility, [40][41][42] International Journal of Nanomedicine downloaded from https://www.dovepress.com/ by 44.224.250.200 on 11-Jul-2020 For personal use only. which was also observed in our experiment ( Figures 3A and 5).…”

Section: Discussionmentioning

confidence: 99%

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Su¹,

Wang²,

Jiang³

et al. 2019

IJN

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Background These normal entheses are not reestablished after repair despite significant advances in surgical techniques. There is a significant need to develop integrative biomaterials, facilitating functional tendon-to-bone integration. Materials and methods We fabricated a highly interconnective graphene oxide-doped electrospun poly(lactide-co-glycolide acid) (GO-PLGA) nanofibrous membrane by electrospinning technique and evaluated them using in vitro cell assays. Then, we established rabbit models, the PLGA and GO-PLGA nanofibrous membranes were used to augment the rotator cuff repairs. The animals were killed postoperatively, which was followed by micro-computed tomography, histological and biomechanical evaluation. Results GO was easily mixed into PLGA filament without changing the three dimensional microstructure. An in vitro evaluation demonstrated that the PLGA membranes incorporated with GO accelerated the proliferation of BMSCs and furthered the Osteogenic differentiation of BMSCs. In addition, an in vivo assessment further revealed that the local application of GO-PLGA membrane to the gap between the tendon and the bone in a rabbit model promoted the healing enthesis, increased new bone and cartilage generation, and improved collagen arrangement and biomechanical properties in comparison with repair with PLGA only. Conclusion The electrospun GO-PLGA fibrous membrane provides an effective approach for the regeneration of tendon to bone enthesis.

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“…In addition to natural polymers such as chitosan, dextran, and albumin, synthetic polymers such as polyesters, polyanhydrides, and polyamides can be mentioned. Mostly studied poly(lactic-coglycolic acid) (PLGA) is a synthetic copolymer composed of lactide and glycolide and approved by the European Medicine Agency (EMA) and US Food and Drug Administration (FDA) [64]. This polymer, which is biodegradable and biocompatible, is suitable for vaccine and drug delivery systems in humans.…”

Section: Peptide Vaccinesmentioning

confidence: 99%

Synthesis and Applications of Synthetic Peptides

Ucar¹,

Acar²,

Arayici³

et al. 2019

Peptide Synthesis

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The synthesis and applications of the peptides are gaining increasing popularity as a result of the developments in biotechnology and bioengineering areas and for a number of research purposes including cancer diagnosis and treatment, antibiotic drug development, epitope mapping, production of antibodies, and vaccine design. The use of synthetic peptides approved by the health authorities for vaccine, for cancer, and in drug delivery systems is increasing with these developments. The aim of this book chapter is to review the recent developments in the use of peptides in the diagnosis of drug and vaccine systems and to present them to the reader with commercially available illustrations.

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Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Cited by 164 publications

References 289 publications

Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity

Encapsulation of the dual FLAP/mPEGS-1 inhibitor BRP-187 into acetalated dextran and PLGA nanoparticles improves its cellular bioactivity

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

Synthesis and Applications of Synthetic Peptides

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