Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function

Shen, He; Chen, Xi; Li, Xing; Jia, Ke Ran; Xiao, Zhifeng

doi:10.1007/s11427-019-9490-8

Cited by 18 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Collagen, as a natural biomaterial, has been used in regenerative therapy such as spinal cord injury repair (Han et al, 2018;Lin, 2019;Shen et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

Wang

et al. 2020

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Stem cell therapy is an attractive approach for recovery from myocardial infarction (MI) but faces the challenges of rapid diffusion and poor survival after transplantation. Here we developed an injectable collagen scaffold to promote the long-term retention of transplanted cells in chronic MI. Forty-five minipigs underwent left anterior descending artery (LAD) ligation and were equally divided into three groups 2 months later (collagen scaffold loading with human umbilical mesenchymal stem cell (hUMSC) group, hUMSC group, and placebo group (only phosphate-buffered saline (PBS) injection)). Immunofluorescence staining indicated that the retention of transplanted cells was promoted by the collagen scaffold. Echocardiography and cardiac magnetic resonance imaging (CMR) showed much higher left ventricular ejection fraction (LVEF) and lower infarct size percentage in the collagen/hUMSC group than in the hUMSC and placebo groups at 12 months after treatment. There were also higher densities of vWf-, α-sma-, and cTnT-positive cells in the infarct border zone in the collagen/cell group, as revealed by immunohistochemical analysis, suggesting better angiogenesis and more cardiomyocyte survival after MI. Thus, the injectable collagen scaffold was safe and effective on a large animal myocardial model, which is beneficial for constructing a favorable microenvironment for applying stem cells in clinical MI. injectable collagen scaffold, mesenchymal stem cell, MI

show abstract

“…Collagen, as a natural biomaterial, has been used in regenerative therapy such as spinal cord injury repair (Han et al, 2018;Lin, 2019;Shen et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

Wang

et al. 2020

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…All animal procedures were performed depending on the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health. The surgical procedure was similar to that previously described, with minor modifications (Han et al, 2018;Shen et al, 2019;Shen et al, 2020). Mice were anesthetized through administration of chloral hydrate (0.5 g kg −1…”

Section: Surgical Proceduresmentioning

confidence: 99%

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Xue

Shu

Xiao

et al. 2021

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Nestin is expressed extensively in neural stem/progenitor cells during neural development, but its expression is mainly restricted to the ependymal cells in the adult spinal cord. After spinal cord injury (SCI), Nestin expression is reactivated and Nestinpositive (Nestin + ) cells aggregate at the injury site. However, the derivation of Nestin + cells is not clearly defined. Here, we found that Nestin expression was substantially increased in the lesion edge and lesion core after SCI. Using a tamoxifen inducible CreER(T2)-loxP system, we verified that ependymal cells contribute few Nestin + cells either to the lesion core or the lesion edge after SCI. In the lesion edge, GFAP + astrocytes were the main cell type that expressed Nestin; they then formed an astrocyte scar.In the lesion core, Nestin + cells expressed αSMA or Desmin, indicating that they might be derived from pericytes. Our results reveal that Nestin + cells in the lesion core and edge came from various cell types and rarely from ependymal cells after complete transected SCI, which may provide new insights into SCI repair.

show abstract

“…Transplanted aSCGs have been shown to survive long‐term at lesion sites and improve functional recovery in rodent and canine SCI models. [ 179 ] A growth factor “cocktail” containing BDNF, NT‐3, and VEGF was applied to the transplants, which enhanced the survival levels of the donor cells and improved the therapeutic efficiency of aSCGs. Adult spinal cord tissue grafts are composed of various types of cells, such as neurons, astrocytes, oligodendrocytes, and endothelial cells, and have a complex architecture and extracellular matrix.…”

Section: Advanced Technologies For Structure and Function Restorationmentioning

confidence: 99%

“…Adult spinal cord tissue grafts are composed of various types of cells, such as neurons, astrocytes, oligodendrocytes, and endothelial cells, and have a complex architecture and extracellular matrix. They can therefore be considered as a promising approach for SCI future repairs [ 178–180 ] ( Figure ).…”

Section: Advanced Technologies For Structure and Function Restorationmentioning

confidence: 99%

Advances in Biomaterial‐Based Spinal Cord Injury Repair

Shen

Fan

You

et al. 2021

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

Spinal cord injury (SCI) often leads to the loss of motor and sensory functions and is a major challenge in neurological clinical practice. Understanding the pathophysiological changes and the inhibitory microenvironment is crucial to enable the identification of potential mechanisms for functional restoration and to provide guidance for the development of efficient treatment and repair strategies. To date, the implantation of specifically functionalized biomaterials in the lesion area has been shown to help promote axon regeneration and facilitate neuronal circuit generation by remolding SCI microenvironments. Moreover, structural and functional restoration of the spinal cord through the transplantation of naive spinal cord tissue grafts from adult donors, artificial spinal cord-like tissue developed from tissue engineering, and 3D printing will open up new avenues for SCI treatment. This review focuses on the dynamic pathophysiological changes in SCI microenvironments, biomaterials for SCI repairs, strategies for restoring spinal cord structure and function, experimental animal models, regenerative mechanisms, and clinical studies for SCI repair. The current status, recent advances, challenges, and prospects of scaffold-based SCI repair from basic to clinical settings are summarized and discussed, to provide a reference that will help to guide the future exploration and development of spinal cord regeneration strategies.

show abstract

Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function

Cited by 18 publications

References 40 publications

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells

Lineage tracing reveals the origin of Nestin-positive cells are heterogeneous and rarely from ependymal cells after spinal cord injury

Advances in Biomaterial‐Based Spinal Cord Injury Repair

Contact Info

Product

Resources

About