Transplantation Antigen Activity of Human Blood Platelets1

Dausset, J; Ft, Rapaport

doi:10.1097/00007890-196603000-00008

Cited by 37 publications

(7 citation statements)

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“…Most patients receiving platelets have acute hematological malignancies and possibly an altered immunological status both due to the disease and its treatment with immunosuppressive drugs (9). Platelets possess isoantigens along with antigens of the HL-A system (10,11). Platelet-specific antigens, which in certain clinical situations are of prime importance, are not detected by HL-A lymphocyte typing (12).…”

Section: Introductionmentioning

confidence: 99%

Detection of platelet isoantibodies by (3H)serotonin platelet release and its clinical application to the problem of platelet matching.

Gockerman¹,

Bowman²,

Conrad³

1975

J. Clin. Invest.

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A B S T R A C T The detection of platelet isoantibodies by the release of ['H]serotonin from platelets has been evaluated. The conditions for optimal release of ['H]serotonin with platelet isoantibodies using a microtechnique have been defined. A group of cardiac surgery patients were followed pre-and post-transfusions, with 48% developing a positive serotonin release assay. Of these patients, 16% also had a platelet complement-fixing and/or lymphocytotoxic isoantibody. There was variation in the degree of correlation between ['H]serotonin release and lymphocytotoxicity using individual National Institutes of Health typing serum. The matching obtained between family members by both techniques showed a close correlation when each technique was evaluated separately using the same NIH typing serum. The detection of isoantibodies in patients with hematological malignancies correlated with the unresponsiveness to unmatched platelet transfusions in 15 out of 17 cases. The use of the patient's isoantibody to matched platelets of family members by ['H]serotonin release correlated well with the clinical response to transfusion with these platelets. The data suggest that (a) platelet isoantibodies can be

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Section: Introductionmentioning

confidence: 99%

Detection of platelet isoantibodies by (3H)serotonin platelet release and its clinical application to the problem of platelet matching.

Gockerman¹,

Bowman²,

Conrad³

1975

J. Clin. Invest.

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“…If platelets have stimulated lymphocytes to transform, they can be used as antigen in MLPR without treatment with Mitomycin C or sOCo -i rradiation, and therefore the platelets can be employed conveniently as antigenic stimulator in one way interaction of MLC. However, as Dausset and Rapaport (1966) and Rapaport (1968) described that much larger doses of platelets than leukocytes were required to induce skin allograft sensitivity on an individual-specific basis; more than 1 X 109 platelets as antigen have been required in the mixed culture to obtain enough counts in our experiments .…”

Section: Discussionmentioning

confidence: 86%

Mixed Lymphocyte-Platelet, Lymphocyte-Kidney Cell and Lymphocyte-Liver Cell Reactions in Rats

Nishihira

Fujii

1972

Tohoku J. Exp. Med.

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“…Alloimmunization, which correlates well with decreased responsiveness to random donor platelets, can be demonstrated by assaying for lymphocytotoxic antibodies directed at HLA-antigens (Daly et al, 1980: Howard & Perkins, 1978Hogge et al 198 3: Schiffer et al, 19 78). For example, there is known to be variable expression of HLA antigens on platelets compared to lymphocytes (Dausset & Rapaport, 1966;Aster et al 1977). However, this assay uses lymphocytes and not platelets as the target cell which may limit the sensitivity and specificity of the assay.…”

Section: Discussionmentioning

confidence: 99%

Refractoriness to random donor platelet transfusions in patients with aplastic anaemia: a multivariate analysis of data from 264 cases

et al. 1987

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Frequent platelet support is an essential part of the management of patients with severe aplastic anaemia and platelet transfusions from random donors are usually given as initial therapy. To evaluate those parameters that might correlate with the development of refractoriness to platelets from random donors, we performed a retrospective multivariate analysis in 264 patients with severe aplastic anaemia who presented for allogeneic bone marrow transplantation. Two hundred and ten (79.5%) of these patients had received multiple platelet and red cell transfusions, and 71 (34%) were refractory to random donor platelets. The strongest factor correlating with refractoriness was the presence of lymphocytotoxic antibodies, followed by the number of platelet units previously transfused. However, the latter variable attained significance only when the number of platelet units transfused exceeded 40. When given HLA-compatible platelet transfusions, only five (7%) of the refractory patients did not show a reasonable post-transfusion platelet increment. Measures which would delay or prevent platelet alloimmunization might include a policy of therapeutic rather than prophylactic platelet transfusions, and referring patients early in the course of their disease for marrow grafting if a suitable donor is available.

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Transplantation Antigen Activity of Human Blood Platelets1

Cited by 37 publications

References 0 publications

Detection of platelet isoantibodies by (3H)serotonin platelet release and its clinical application to the problem of platelet matching.

Detection of platelet isoantibodies by (3H)serotonin platelet release and its clinical application to the problem of platelet matching.

Mixed Lymphocyte-Platelet, Lymphocyte-Kidney Cell and Lymphocyte-Liver Cell Reactions in Rats

Refractoriness to random donor platelet transfusions in patients with aplastic anaemia: a multivariate analysis of data from 264 cases

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