Transovarial Transmission of Dengue 2 Virus by Aedes Aegypti in Nature

Khin, Mi Mi; Than, Khin Aye

doi:10.4269/ajtmh.1983.32.590

Cited by 95 publications

(63 citation statements)

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“…En 1983, Khin, et al, recolectaron en campo larvas y mosquitos machos, y reportaron que estos individuos eran positivos para DENV-2 (20). Así, se confirmó la competencia de A. aegypti como vector al permitir la producción de grandes cantidades de virus y su circulación en los diferentes tejidos y órganos del insecto, incluido el aparato reproductor, lo cual favorece la transmisión vertical del virus a su progenie.…”

Section: Discussionunclassified

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

et al. 2017

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Introducción. La incidencia y la prevalencia del dengue en Cundinamarca son elevadas y, recientemente, se detectó Aedes aegypti en algunas áreas rurales del departamento.Objetivo. Evaluar la transmisión transovárica del virus del dengue en larvas y pupas recolectadas en áreas rurales del municipio de Anapoima.Materiales y métodos. Se recolectaron ejemplares vivos en 53 viviendas y se transportaron al laboratorio de Anapoima, donde se clasificaron, se agruparon y se congelaron. Llevadas a Bogotá, se las homogeneizó, se les extrajo el ARN con Trizol®, se las sometió a una reacción en cadena de la polimerasa de transcripción inversa (Reverse Transcription Polymerase Change Reaction, RT-PCR) y a PCR convencional, y los productos amplificados se analizaron en geles de agarosa al 2 %.Resultados. En 54,7 % de las viviendas evaluadas se encontraron formas inmaduras del vector y el serotipo más frecuente fue el DENV-1. Sin embargo, en algunos pools se detectó la presencia simultánea de los serotipos DENV 1 y 2, DENV 1 y 3, y DENV 1 y 4, así como los serotipos DENV 1, 2 y 3.Conclusión. Los resultados confirmaron la transmisión vertical del virus de manera natural en el área rural del municipio, lo cual reafirma la capacidad vectorial de A. aegypti y explica, en parte, la persistencia del virus en la región y la posibilidad de que en la fase adulta el vector lo transmita sin haber consumido sangre infectada. Esta situación aumenta el riesgo de infección por el virus del dengue en Colombia y, por lo tanto, la necesidad de adelantar programas de prevención y control en todas las zonas con presencia del mosquito.

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Section: Discussionunclassified

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

et al. 2017

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“…aegypti. [5][6][7] According to Joshi et al, 6 transovarial transmission allows dengue virus to persist in successive generations of mosquitoes, at rates of 5% to 26% in the lab, although rates in nature are likely not to exceed 20%.…”

Section: Discussionmentioning

confidence: 99%

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Zeidler¹,

Acosta²,

Barrêto³

et al. 2008

Rev. Saúde Pública

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OBJECTIVE:To detect the presence of dengue virus in larval forms of Aedes aegypti and to associate vector presence with rainfall and incidence of disease. METHODS:Eighteen households were randomly selected for egg collection in a neighborhood of the city of Boa Vista, Roraima, in Northern Brazil. Two oviposition traps were installed per home, and removed after one week. This was repeated on a monthly basis between November 2006 and May 2007. Trap positivity rate and egg density were calculated. Following the eclosion of 1,422 eggs, 44 pools of at least 30 larvae each were formed, which were evaluated for presence of dengue virus using RT-PCR and hemi-nested PCR. Dengue incidence rates in the period were correlated with rainfall rates. The association between these two variables and the number of eggs collected was determined using Pearson correlation. RESULTS:None of the pools tested positive for presence of dengue virus, despite the high incidence of dengue in the neighborhood during the studied period. The density of Ae. aegypti increased with rainfall, but was not correlated with incidence of dengue. CONCLUSIONS:The results suggest that transovarial transmission of dengue virus in mosquitoes occurs at a very low frequency, and therefore virus persistence in urban settings may not depend on such transmission. The mosquito population increased during the rainy season due to increased formation of breeding sites; the lack of correlation with incidence of dengue may be due to underestimation of incidence data during epidemics.

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“…6,7 Many studies have shown that DENV could be transovarially transmitted to progeny mosquitoes both in the laboratory and field. [50][51][52][53][54][55][56][57][58][59][60] It was considered to be an important means for the maintenance of DENV in nature. However, the mechanism of transovarially transmission is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

Zhang

Zheng²,

Yu³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Dengue virus serotype 2 (DENV-2) RNA replication profiles and tropisms were studied by using quantitative RT-PCR (q-RTPCR) in intrathoracically infected Aedes albopictus. The virus RNA replication profiles were diverse in mosquito organs. In fat body, brain, salivary gland, and malpighian tubes, it peaked at 8, 23, 23, and 27 days post-infection, respectively, and then, all declined. In midgut, it increased all the time and had no trend of decline. In ovary, it had no apparent increase. Subsequent Western blotting of DENV-2 E protein had similar results. Using ribosomal protein 7 (rpS7) as an internal control, we found that, in salivary gland, brain, fat body, and midgut, the average DENV-2 RNA levels (DENV-2 RNA/rpS7 mRNA) were 1,028, 464, 5.6, and 6.2, respectively; in malpighian tubes, it was 1, and in ovary, it was far less than 1. These results suggest that infection profiles and tropism of DENV-2 RNA in Ae. albopictus organs are significantly different.

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Transovarial Transmission of Dengue 2 Virus by Aedes Aegypti in Nature

Cited by 95 publications

References 0 publications

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Vírus dengue em larvas de Aedes aegypti e sua dinâmica de infestação, Roraima, Brasil

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

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