Transmitted Antiretroviral Drug Resistance and Thumb Subdomain Polymorphisms Among Newly HIV Type 1 Diagnosed Patients Infected with CRF01_AE and CRF07_BC Virus in Guangdong Province, China

Abstract

The aim of this study was to elucidate the prevalence of transmitted drug-resistant (TDR) mutations and reverse transcriptase (RT) thumb subdomain polymorphisms in CRF01_AE and CRF07_BC virus among newly diagnosed, therapy-naive HIV-1 patients in Guangdong Province, China. One hundred and sixty-four samples were collected in the Guangzhou Eighth People's Hospital. The entire protease gene and 300 codons of the entry part of the reverse transcriptase were amplified and sequenced. Furthermore, genotypic drug resistance, polymorphisms, and their phylogeny were analyzed. According to eligibility criteria, seven samples were excluded, and 119 of 157 (75.8%) samples (84 CRF01_AE and 35 CRF07_BC) were amplified and sequenced successfully. The prevalence of TDR identified in the present study was 6.7% [8/119, 95% confidence interval (CI) 1.8-11.6%]. Three major resistance mutations, K103N, M184V, and Y188L, each of which caused more than one drug resistance, appeared in only two patients; the prevalence [1.7 % (2/119)] was relatively low. Until now, this is the first observation of the five newly identified accessory mutations, V35T, K43E, V60I, K122E, and E203D, and seven thumb subdomain polymorphisms, A272P, K277R, K281R, T286A, E291D, V292I, and I293V, in the RT gene in China. These findings provide useful information for guidance on the antiretroviral therapy (ART) policy in China where therapeutic options are still limited.

Identification of 3 Distinct HIV-1 Founding Strains Responsible for Expanding Epidemic Among Men Who Have Sex With Men in 9 Chinese Cities

“…1A). Similar to CRF01_AE cluster 1, none of the CRF07_BC sequences available in the database (n = 487) from non-MSM high-risk populations belonged to CRF07_BC cluster 3 except for a few CRF07_BC strains from Guangdong IDUs (6 of 35)32 (Fig. 1B).…”

Prevalence of Transmitted HIV-1 Drug Resistance Remains Low in Guangxi, China, Eight Years after Scale-Up of Highly-Active Antiretroviral Therapy

“…A survey from the prefecture reveals that the prevalence of TDR was 4.3% after 7 years of the scale-up [19]. Another study from Guangdong province showed that the prevalence of TDR was 6.7% after 5 years of the scale-up [20]. A report from Henan province, the first province to provide free ARV to HIV-infected individuals in 2003 in China, shows that the prevalence of TDR in the province is 14.3%, 8 years after the scale-up [21].…”

Targeting HIV Prevention Based on Molecular Epidemiology Among Deeply Sampled Subnetworks of Men Who Have Sex With Men

“…Participants were identified with primary infection by screening 2000 MSM for HIV infection every 2 months using enzyme-linked immunosorbent assay for antibody testing and nested polymerase chain reaction for HIV RNA testing [2,7]. All participants were diagnosed with HIV within 2 months of their infection and had their blood collected for CD4 T-cell count, viral load measurements, and HIV genotyping [2,5,8]. Sequences covered a 1.3-kb region of the HIV-1 pol, including protease (amino acids 1-99) and partial reverse transcriptase (amino acids 1-305; GenBank accession numbers KP250654-KP250829).…”