Transmission of Barley Yellow-Dwarf Virus by Four Biotypes of the Corn Leaf Aphid, Rhopalosiphum maidis1

Saksena, K.N.; Singh, S.R.K.; Sill, W. H.

doi:10.1093/jee/57.4.569

Cited by 29 publications

(21 citation statements)

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“…Most studies on aphid-BYDV relationships have been focused on variation in species specific transmission among aphid species but inter-clonal variation for BYDV transmission has also been assessed. In the USA, Rochow (1960) described clones of the greenbug Schizaphis graminum (Rondani) differing in their ability to transmit a BYDV-SGV isolate, and four biotypes (clones differing by biological characters) of the corn leaf aphid Rhopalosiphum maidis (Fitch) were reported to differ in their ability to transmit the AG-1 strain of BYDV (Saksena et al, 1964). Rochow and Eastop (1966) reported variation in the abilities of two morphologically different clones of Rhopalosiphum padi (L) to transmit BYDV-RMV, and noticed that differences between clones were less pronounced when experiments were conducted at 30°C than at lower temperatures.…”

Section: Introductionmentioning

confidence: 99%

Intra-specific variation and inheritance of BYDV-PAV transmission in the aphid Sitobion avenae

et al. 2005

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Vector efficiency of 44 clonal lines (clones) of Sitobion avenae belonging to 31 different genotypes (distinct patterns for five microsatellite loci) originating from Western France was evaluated by transmitting the isolate PAV4 of BYDV-PAV to barley seedlings. Variation in transmission rates from 3.7% to 92.5% was observed, with significant effects of the aphid clone, of the plant species on which clones were collected, and of the reproductive mode of the clones. When genotypes are considered instead of clones, a continuum in transmission rates was observed. A subset of S. avenae clones was tested for transmission of one (10 clones) and 13 (4 clones) other BYDV-PAV isolates, and a clear clone effect modulated by an isolate effect was observed. Crosses were made between clones with different vectoring phenotypes and their F1 progeny were tested for PAV4 transmission. The narrow sense heritability of the PAV transmission character was rather high in the F1 families (h 2 ¼ 0.5) and the segregation analyses suggested an oligo/polygenic determinism of this character. The possibility of generating new transmission variants by sexual reproduction and its consequences on transmission mechanism studies and on BYD epidemics are discussed.

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Section: Introductionmentioning

confidence: 99%

Intra-specific variation and inheritance of BYDV-PAV transmission in the aphid Sitobion avenae

et al. 2005

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“…Intraspecific variation has been investigated for non-persistently (stylet-borne) transmitted viruses (Sohi & Swenson, 1964;Upreti & Nagaich, 1971b;Thottappilly et al, 1972Thottappilly et al, , 1977Jurik et al, 1980;Singh et al, 1983;Lupoli et al, 1992) and persistently (circulative) transmitted viruses (Björling & Ossiannilsson, 1958;Saksena et al, 1964;Rochow & Eastop, 1966;Robert & Maury, 1970;Upreti & Nagaich 1971a). For most of these virus-vector combinations, variations between transmitting abilities of different clones were found.…”

Section: Introductionmentioning

confidence: 99%

“…Most studies of aphid-BYDV relationships have been focused on variation in species-specific transmission among aphid species but clonal variations for serotype transmission were demonstrated even in the earlier studies. In the USA, Rochow (1960) reported strains of the greenbug Schizaphis graminum differing in their ability to transmit a BYDV-SGV isolate and four biotypes of the corn leaf aphid Rhopalosiphum maidis were proved to differ in their ability to transmit the AG-1 strain of BYDV (Saksena et al, 1964). Rochow & Eastop (1966) also reported variations in transmission abilities of two morphologically distinct clones of Rhopalosiphum padi for BYDV-RMV, and noticed that differences were less pronounced when experiments were carried out at 30ЊC than at lower temperatures.…”

Section: Introductionmentioning

confidence: 99%

Role of acquisition and inoculation time in the expression of clonal variation for BYDV‐PAV transmission in the aphid species Rhopalosiphum padi

Sadeghi¹,

Dedryver²,

Gauthier³

1997

Plant Pathology

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Vector efficiency of 20 Rhopalosiphum padi clones, originating from Europe, North America and North Africa and exhibiting different types of life cycle, was evaluated by transmitting a French BYDV-PAV isolate to barley plants under five different acquisition/inoculation sequences (AAP/IAP). Differences between clones in transmission efficiency were found only when a short AAP was followed by a long IAP (6 h/120 h) and, to some extent, when a long AAP (48 h) was followed by a short IAP (6 h), but no differences were found when the conditions for virus transmission were optimal, i.e. long AAP followed by long IAP (48 h/120 h). There were no differences in transmission rates by clones of different geographical origins and with different life cycles. As a consequence, clonal variation is probably of little importance in the vector aspects of the epidemiology of PAV serotypes transmitted by R. padi, but the availability of a range of clones exhibiting transmission differences under limiting AAP or IAP conditions could be of interest for studies of virus-vector relationships.

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“…Most studies in the virus-aphid relationships of BYDV have been focused on virus variation associated with the specific transmission by different cereal aphid species. A few studies have compared the variation of vectoring ability among aphid clones within vector species (Saksena, Singh & Sill, 1964;Rochow & Eastop, 1966: Gildow & Rochow, 1983. A better understanding of the variation in vectoring ability hetween aphid clones within species will be critical to the genetic analysis of transmission mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Vectoring ability of aphid clones of Rhopalosiphum padi (L.) and Sitobion avenae (Fabr.) and their capacity to retain barley yellow dwarf virus

Jing-Quan

Lapierre

Moreau

1997

Annals of Applied Biology

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Vectoring ability of four aphid clones, Rp-M and Rp-R26 of Rhopalosiphum padi and Sa-RI and Sa-V of Sitobion avenae, to transmit barley yellow dwarf (PAV, MAV and RPV) luteoviruses (BYDV) was compared in controlled conditions. Significant differences between highly efficient vectors (HEV), Rp-M and Sa-R1, and poorly efficient vectors (PEV), Rp-R26 and Sa-V, were found in transmission of their specific viruses with acquisition and inoculation access periods (AAP, IAP) of 5 days. BYD-RPV was occasionally transmitted by both clones of S. avenae. None of 150 tested apterous adults of the Rp-R26 transmitted BYD-MAV, while 10% of transmission was observed from those of the Rp-M in a parallel test. An improved ELISA and immuno-PCR were adapted to test for viruses in aphids. The results obtained by the improved ELISA indicated there was a good correlation between virus detection in single aphids of HEV clones after a 5 day AAP and virus transmission by them. In contrast, the percentages of viruscarrying aphids of PEV clones were generally higher than those of their transmission rates. BYD-MAV and BYD-RPV were also detected by the improved ELISA in single aphids of their PEV clones, with the exception of BYD-RPV in those of Sa-V. However, after a 2-day IAP, the improved ELISA in most cases failed to detect these viruses in single aphids of PEV clones.Detection by immuno-PCR demonstrated that all three viruses could be acquired and retained by the aphids of both HEV and PEV clones. But, as visualised from electrophoretic bands, after the 2-day IAP the amplified products from aphid extracts of PEV clones were reduced. The detection in a batch of nine aphids by the improved ELISA revealed that virus content in PEV clones decreased more rapidly than that in HEV clones during transmission. Thus, the difference in transmission efficiency of the aphid clones within species was not caused by an inability to acquire virus, but was determined by variation in vectoring ability between them. This was due to differences in ability to prevent the passage of virions from haemocoel to salivary duct and/or different capacities for the retention of BYDV. 1997 Association of Applied Biologists 180 GUO JING-QU AN, H E R V~ LAPERM AND JEAN-PIERRE MOFEAU

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Transmission of Barley Yellow-Dwarf Virus by Four Biotypes of the Corn Leaf Aphid, Rhopalosiphum maidis1

Cited by 29 publications

References 0 publications

Intra-specific variation and inheritance of BYDV-PAV transmission in the aphid Sitobion avenae

Intra-specific variation and inheritance of BYDV-PAV transmission in the aphid Sitobion avenae

Role of acquisition and inoculation time in the expression of clonal variation for BYDV‐PAV transmission in the aphid species Rhopalosiphum padi

Vectoring ability of aphid clones of Rhopalosiphum padi (L.) and Sitobion avenae (Fabr.) and their capacity to retain barley yellow dwarf virus

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