Transmission geometry laser ablation into a non‐contact liquid vortex capture probe for mass spectrometry imaging

Ovchinnikova, Olga S.; Bhandari, Deepak; Lorenz, Matthias; Berkel, Gary J. Van

doi:10.1002/rcm.6946

Cited by 38 publications

(58 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No obvious relationship was found between the transfer efficiency and peptide properties such as amino acid composition or compound hydrophobicity. The measured transfer efficiency of 16% is consistent with previous estimates between 10% and 20% [29,39] but higher than our previously reported values, suggesting improvement in capture efficiency because of the vacuum.…”

Section: Resultssupporting

confidence: 92%

“…In a work by Ovchinnikova and coworkers [39], laser ablation in transmission mode using a 355 nm UV laser with the plume was captured in a liquid microjunction resulted in a transfer efficiency of 10%. The use of a vortex probe instead of a liquid microjunction improved this value to 24% [29] using a commercial dye standard.…”

Section: Resultsmentioning

confidence: 99%

“…We have recently used the technique of laser ablation sample transfer (LAST) in conjunction with MSI for the analysis of lipids in rat brain tissue sections [27]. Laser ablation sample transfer using UV and IR lasers has been coupled with liquid capture and mass spectrometry, displaying the ability to transfer and detect large biomolecules [28,29]. In particular, IR lasers have been used in several studies [30][31][32] because of their high material removal efficiency.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Laser Ablation with Vacuum Capture for MALDI Mass Spectrometry of Tissue

Donnarumma

Cao

Murray

2015

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Abstract. We have developed a laser ablation sampling technique for matrix-assisted laser desorption ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS) analyses of in-situ digested tissue proteins. Infrared laser ablation was used to remove biomolecules from tissue sections for collection by vacuum capture and analysis by MALDI. Ablation and transfer of compounds from tissue removes biomolecules from the tissue and allows further analysis of the collected material to facilitate their identification. Laser ablated material was captured in a vacuum aspirated pipette-tip packed with C18 stationary phase and the captured material was dissolved, eluted, and analyzed by MALDI. Rat brain and lung tissue sections 10 μm thick were processed by in-situ trypsin digestion after lipid and salt removal. The tryptic peptides were ablated with a focused mid-infrared laser, vacuum captured, and eluted with an acetonitrile/ water mixture. Eluted components were deposited on a MALDI target and mixed with matrix for mass spectrometry analysis. Initial experiments were conducted with peptide and protein standards for evaluation of transfer efficiency: a transfer efficiency of 16% was obtained using seven different standards. Laser ablation vacuum capture was applied to freshly digested tissue sections and compared with sections processed with conventional MALDI imaging. A greater signal intensity and lower background was observed in comparison with the conventional MALDI analysis. Tandem time-of-flight MALDI mass spectrometry was used for compound identification in the tissue.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Laser Ablation with Vacuum Capture for MALDI Mass Spectrometry of Tissue

Donnarumma

Cao

Murray

2015

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…Since then, a number of other ambient MSI modalities have also been developed that utilize different ambient ionization techniques to create MS images. The variety of methods include laser-based techniques such as laser ablation electrospray ionization (LAESI) MS [15] and transmission geometry laser ablation MSI [16], spray-based techniques such as DESI MS and electrostatic spray ionization (ESTASI) MS [17], and surface micro-extraction-based methods such as LMJ-SSP MS [18], liquid extraction surface analysis (LESA) MS [19], and nano-DESI MS [20], among many other emerging technologies that are actively being researched at the present time [21].…”

Section: Introductionmentioning

confidence: 99%

“…Transmission geometry laser ablation MSI uses a laser to ablate material from a surface into a liquid vortex capture probe that is coupled with the ESI source of a mass spectrometer. The resolution achievable with this method is around 50 μm on a mouse brain section [16]. The DESI method sprays charged solvent droplets at the sample surface to generate the secondary ions detected by MS analysis, and it has been able to achieve a spatial resolution of 35 μm [22] on biological tissues.…”

Section: Introductionmentioning

confidence: 99%

High Resolution Tissue Imaging Using the Single-probe Mass Spectrometry under Ambient Conditions

Rao

Pan

Yang

2015

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Abstract. Ambient mass spectrometry imaging (MSI) is an emerging field with great potential for the detailed spatial analysis of biological samples with minimal pretreatment. We have developed a miniaturized sampling and ionization device, the Singleprobe, which uses in-situ surface micro-extraction to achieve high detection sensitivity and spatial resolution during MSI experiments. The Single-probe was coupled to a Thermo LTQ Orbitrap XL mass spectrometer and was able to create high spatial and high mass resolution MS images at 8±2 and 8.5 μm on flat polycarbonate microscope slides and mouse kidney sections, respectively, which are among the highest resolutions available for ambient MSI techniques. Our proof-of-principle experiments indicate that the Single-probe MSI technique has the potential to obtain ambient MS images with very high spatial resolutions with minimal sample preparation, which opens the possibility for subcellular ambient tissue MSI to be performed in the future.

show abstract