Transmission events and antimicrobial susceptibilities of methicillin-resistant Staphylococcus argenteus in Stockholm

Abstract

Objectives: Staphylococcus argenteus has been increasingly reported since the species was defined as a novel staphylococcal species in 2015. This study aims to investigate genetic epidemiological links and antimicrobial susceptibilities of methicillin-resistant S. argenteus isolates recovered in Stockholm. Methods: Sixteen methicillin-resistant S. argenteus isolates were identified from a collection of methicillin-resistant Staphylococcus aureus in Stockholm 2007e2018, by using whole-genome sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The genomes of the isolates were investigated by pulsed-field gel electrophoresis, singlenucleotide polymorphism (SNP)-based phylogeny, k-mer analysis, core-genome multi-locus sequence typing (cgMLST), resistance traits and virulence factors. The MICs of 19 antimicrobial agents for each isolate were determined by using the broth microdilution method. Results: Of the 16 isolates, seven, seven and two isolates were assigned to ST1223, ST2250 and ST2793, respectively, with the S. aureus MLST-scheme. Analyses based on SNPs and cgMLST revealed a likely clonal spread of methicillin-resistant S. argenteus in 2007. Four isolates were found to be resistant to nonb-lactams in antimicrobial susceptibility testing.Conclusions: A transmission event of methicillin-resistant S. argenteus in family was identified by this study. Among our limited number of isolates, non-b-lactam resistance was detected, which highlights the necessity of a continued surveillance on this emerging pathogen. S. argenteus could be correctly identified by MALDI-TOF MS with the updated database, enabling its detection also in clinical laboratories.

Staphylococcus argenteus as an etiological agent of prosthetic hip joint infection: a case presentation

“…From Europe the reports are sparse; 25 S. argenteus genomes from Denmark [8], low prevalence (0.16%) in a nationwide Belgian study [9], a case report from France [10] and one strain from UK [3]. However, among isolates classically identified as methicillin-resistant S. aureus (MRSA) in Sweden that is normally considered a low prevalence country regarding MRSA, S. argenteus has also been identified [11,12].…”

Heterogeneity of Molecular Characteristics among Staphylococcus argenteus Clinical Isolates (ST2250, ST2793, ST1223, and ST2198) in Northern Taiwan

“…To date, characteristics such as the lack of pigment have been applied to identify S. argenteus along with multilocus sequence typing (MLST), rpoB , nonribosomal peptide synthetase ( NRPS ) gene molecular typing, and/or MALDI-TOF MS [ 5 , 7 , 9 ]. Recently, molecular characterization of whole genome sequencing or genotyping of S. argenteus with more than ten clinical isolates of S. argenteus have been reported in Denmark, Thailand, China, Sweden, and Japan [ 10 , 11 , 12 , 13 , 14 ]. Previous studies report the prevalence rate of S. argenteus as ranging from <1% in European countries and Japan to 19% of community onset Staphylococcus sepsis in Thailand [ 10 , 14 , 15 ].…”

“…Previous studies report the prevalence rate of S. argenteus as ranging from <1% in European countries and Japan to 19% of community onset Staphylococcus sepsis in Thailand [ 10 , 14 , 15 ]. ST2250 lineage is the predominant clone and is found worldwide [ 10 , 11 , 12 , 13 , 14 ]. The second most prevalent is ST1223, and its prevalence rate is apparently lower than ST2250 [ 14 ].…”

Genetic Profiling of Methicillin-resistant Staphylococcus aureus in An African Hospital by Multiplex-PCR

“…The PCR product (Amplicons) was electrophoresed on a 1.5% agarose gel prestained with ethidium bromide. A 100 bp plus ladder was loaded in the in the first well using 10x Qiagen™ loading dye [12]. The agarose gel was transferred into Bio-Rad gel documentation device and viewed using a UV trans-illuminator.…”