Transmission Electron Microscope Observation of Epithelial Cells with Single Cilia in Intrahepatic Biliary Ductules of Bats

Tanuma, Yutaka; Ohata, Masako

doi:10.1679/aohc1950.41.367

Cited by 15 publications

(9 citation statements)

References 14 publications

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“…Confirming the findings by CRABO (1963), DE KRETSER (1967a, RUSSO and DE ROSAS (1971), CONNELL (1972), CONNELL and CHRISTENSEN (1975 and USUI (1976), the present author demonstrated a cilium developing from one of the paired centrioles (diplosome). Neither USUI (1976) nor the present author could reveal the fibril pattern of the cilium, but according to Connell it shows a 9+0 pattern, favoring the view that the single cilium of the Leydig cell might be sensory or chemoreceptor in nature (TANUMA and OHATA, 1978).…”

Section: Centriole and Solitary (Single) Ciliumcontrasting

confidence: 68%

“…The fine structures of the macrophages in the interstitial tissue of adult mice agreed almost completely with those described by USUI and WING and LIN containing especially abundant lysosomes and tubular SER with dense material in them. But complex channels described by WING and LIN which were continuous with the extracellular space and which resembled the so-called "worm-like structure" of Kupffer cells of the liver (TANUMA, 1978) were missed.…”

Section: Macrophagementioning

confidence: 99%

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Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Ohata

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Centriole and Solitary (Single) Ciliumcontrasting

confidence: 68%

Section: Macrophagementioning

confidence: 99%

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Ohata

1979

Arch. Histol. Jap., Arch. Histol. Jpn

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“…As proposed by TANUMA and OHATA (1978a) in the hat, the number of profiles of the ciliary shafts in bile ductular lumina generally surpasses that of the basal bodies also detected in the epithelial cells in the bird. This may be accounted for by the fact that there are some unexpectedly long solitary cilia possibly derived from the epithelial cells far outside the section plane.…”

Section: Discussionmentioning

confidence: 60%

“…Single Cilium in Intrahepatic Biliary Duct 297 unrelated to cilium within the intrahepatic biliary epithelium (STEINER et al, 1962;TANUMA and OHATA, 1978a GRISHAM et al (1975) reported that they could count more numerous cilia by SEM than by TEM.…”

Section: Discussionmentioning

confidence: 99%

“…Cilia with the 9+0 fiber pattern in the ciliary shaft and the basal organization of the two-centriole type are believed to be sensory (BARNES, 1961;CURRIE and WHEATLEY, 1966). Concerning the hepatic single cilia found in the intrahepatic biliary passage, the fiber pattern and/or the type of basal structure has been discussed by a number of authors (STEINER et al, 1962;GRISHAM and PORTA, 1963;GRISHAM, 1963;STERNLIEB, 1965;BROOKS et al, 1975;TANUMA and OHATA, 1978a). The work of GRISHAM and PoRTA (1963) seems exceptional, since they reported that the hepatic single cilia showed a 9+2 fiber pattern and was provided with a basal structure of the one-centriole type consistent with typical kinocilia, although in another report GRISHAM (1963) altered his view describing the basal apparatus of two-centriole type.…”

Section: Discussionmentioning

confidence: 99%

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Electron microscopic observations on single cilia in the intrahepatic biliary ducts in some birds.

Ohata

Tanuma

Uchida

1982

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary. The intrahepatic biliary passage in five species of birds was investigated with the transmission electron microscope. The avian liver was characterized by a frequent occurrence of intralobular bile ductules and canaliculo-ductular junctions in the parenchyme. It was further characterized by solitary bile ductular epithelial cells intercalated among hapatocytes surrounding bile canaliculi.The present study first revealed that avian bile ductular epithelial cells possess a long single cilium. Its basal body (distal centriole) was connected to a basal foot and slender rootlet and accompanied by a proximal centriole. The hepatocytes facing the bile passage possessed no cilium, although they frequently had a diplosome in their apical cytoplasm. The single cilia of the bile ductular epithelium gradually tapered toward the tip. The original fiber pattern in the most proximal part was peripheral 9 doublets +0. In the ciliary shaft, the doublets altered into ringlets which were diminished in number gradually toward the distal parts of the shaft, so that in the tip only one singlet remained. Since these fiber patterns in the single cilia markedly deviated from the 9+2 fiber pattern of the ordinary motile cilia, they may not be motile, but properly regarded as sensory or chemoreceptors.Epithelial cells provided with single cilia have been demonstrated by many authors with transmission (TEM) and scanning electron microscopes (SEM) in normal and artificially proliferated intrahepatic biliary ducts in the rat and human (STEINER et al., 1962; STEINER and CARRUTHERS, 1963;GRISHAM, 1963;GRISHAM and PORTA, 1963;STERNLIEB, 1965). In their SEM observations, MOTTA and FUMAGALLI (1974), BROOKS et al. (1975) and GRISHAM et al. (1975) reported the actual length, number and orientation of the single cilia originating from the ductular epithelium in the rat liver. Recently, TANUMA and OHATA (1978a) have observed with TEM the single cilia in the intrahepatic biliary ducts of bats; they demonstrated the arrangement of ciliary microtubules and the pattern of the basal structures.In the course of our TEM study on normal livers in several species of birds, we occasionally found single cilia in the lumen of biliary ducts. We carried out a detailed investigation on them, with the following findings.285

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Reduced primary cilia length and altered Arl13b expression are associated with deregulated chondrocyte Hedgehog signaling in alkaptonuria

Thorpe

Gambassi

Thompson

et al. 2017

Journal Cellular Physiology

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Alkaptonuria (AKU) is a rare inherited disease resulting from a deficiency of the enzyme homogentisate 1,2‐dioxygenase which leads to the accumulation of homogentisic acid (HGA). AKU is characterized by severe cartilage degeneration, similar to that observed in osteoarthritis. Previous studies suggest that AKU is associated with alterations in cytoskeletal organization which could modulate primary cilia structure/function. This study investigated whether AKU is associated with changes in chondrocyte primary cilia and associated Hedgehog signaling which mediates cartilage degradation in osteoarthritis. Human articular chondrocytes were obtained from healthy and AKU donors. Additionally, healthy chondrocytes were treated with HGA to replicate AKU pathology (+HGA). Diseased cells exhibited shorter cilia with length reductions of 36% and 16% in AKU and +HGA chondrocytes respectively, when compared to healthy controls. Both AKU and +HGA chondrocytes demonstrated disruption of the usual cilia length regulation by actin contractility. Furthermore, the proportion of cilia with axoneme breaks and bulbous tips was increased in AKU chondrocytes consistent with defective regulation of ciliary trafficking. Distribution of the Hedgehog‐related protein Arl13b along the ciliary axoneme was altered such that its localization was increased at the distal tip in AKU and +HGA chondrocytes. These changes in cilia structure/trafficking in AKU and +HGA chondrocytes were associated with a complete inability to activate Hedgehog signaling in response to exogenous ligand. Thus, we suggest that altered responsiveness to Hedgehog, as a consequence of cilia dysfunction, may be a contributing factor in the development of arthropathy highlighting the cilium as a novel target in AKU.

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Transmission Electron Microscope Observation of Epithelial Cells with Single Cilia in Intrahepatic Biliary Ductules of Bats

Cited by 15 publications

References 14 publications

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Electron microscopic observations on single cilia in the intrahepatic biliary ducts in some birds.

Reduced primary cilia length and altered Arl13b expression are associated with deregulated chondrocyte Hedgehog signaling in alkaptonuria

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