Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Ohata, Masako

doi:10.1679/aohc1950.42.51

Cited by 28 publications

(12 citation statements)

References 44 publications

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“…Sildenafil treated Leydig cells showed a vesicular SER and some large vacuoles scattered throughout the cytoplasm that are features of an active secreting cell. According to Ohata (1979), these vacuoles are derived from testosterone-containing vesicles of the SER and could migrate towards the cell surface to open into the extra-cellular space.…”

Section: Discussionmentioning

confidence: 99%

Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

Saraiva

Silva

Wanderley³

et al. 2009

Int J Experimental Path

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The phosphodiesterase type 5 (PDE5) inhibitor, Sildenafil, is a novel, oral treatment approach for pulmonary hypertension. As Leydig cells present PDE5, this study was conducted to investigate the effects of the chronic treatment with Sildenafil (25 mg/kg) on male Swiss Webster mice steroidogenesis. After a 4-week long experimental design, Leydig cells were analysed by morphological and immunocytochemical procedures. Serum testosterone was assayed by radioimmunoassay. Leydig cells presented noteworthy ultrastructural alterations, such as a vesicular smooth endoplasmic reticulum, large vacuoles scattered through the cytoplasm, enlarged mitochondria with discontinue cristaes and whorle membranes with vesicles at the periphery, which are typical characteristics of an activated steroid-secreting cell. Important immunocytochemical labelling for steroidogenic acute regulatory protein, cytochrome P450 side-chain cleavage enzyme and testosterone were detected in isolated Leydig cells. In addition, Sildenafil-treated mice showed significant increased levels of total testosterone. The results obtained in the present study are consistent with the hypothesis that the accumulation of cyclic guanosine monophosphate by PDE5 inhibition could be involved in the androgen biosynthesis stimulation. Important clinical implications of hormonal disorders should be taken into account for patients with pulmonary hypertension.

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Section: Discussionmentioning

confidence: 99%

Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

Saraiva

Silva

Wanderley³

et al. 2009

Int J Experimental Path

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“…Features of adult-type Leydig cells included a dense cytoplasmic matrix, an abundance of smooth endoplasmic reticulum, the presence of whorled endoplasmic reticulum, and a relative paucity of glycogen [15,16] (Fig. 3A).…”

Section: Electron Microscopic Observations Of Testicular Morphologymentioning

confidence: 99%

Desert hedgehog (Dhh) Gene Is Required in the Mouse Testis for Formation of Adult-Type Leydig Cells and Normal Development of Peritubular Cells and Seminiferous Tubules

Clark¹,

Garland²,

Russell³

2000

Biology of Reproduction

355

261

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Testes from adult and prepubertal mice lacking the Desert hedgehog (DHH:) gene were examined in order to describe further the role of Dhh in spermatogenesis because, in a previous report, DHH:-null male mice were shown to be sterile. Dhh is a signaling molecule expressed by Sertoli cells. Its receptor, patched (Ptc), has been previously localized to Leydig cells and is herein described as being localized also to peritubular cells. Two phenotypes of the mice were observed: masculinized (7.5% of DHH:-null males) and feminized (92.5%), both of which displayed abnormal peritubular tissue and severely restricted spermatogenesis. Testes from adult feminized animals lacked adult-type Leydig cells and displayed numerous undifferentiated fibroblastic cells in the interstitium that produced abundant collagen. The basal lamina, normally present between the myoid cells and Sertoli cells, was focally absent. We speculate that the abnormal basal lamina contributed to other characteristics, such as extracordal gonocytes, apolar Sertoli cells, and anastomotic seminiferous tubules. The two DHH:-null phenotypes described have common peritubular cell defects that may be indicative of the essential role of peritubular cells in development of tubular morphology, the differentiation of Leydig cells, and the ultimate support of spermatogenesis.

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“…In the clusters, cell contacts are numerous and elaborate. Typical ring-shaped gap junctions are also observed in Leydig cells of mouse and dog (Masako Ohato, 1979;Connell and Christensen, 1975;Larsen, 1977). Gap junctions are seen less frequently at 52 and 62 days p.c.…”

Section: Discussionmentioning

confidence: 89%

Leydig cell development of pig testis in the early fetal period: An ultrastructural study

Vorstenbosch¹,

Colenbrander²,

Wensing³

1982

Am. J. Anat.

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Leydig cell development in the pig testis occurs in three periods (an early fetal, the perinatal period, and the period from puberty onward). The earliest of these periods was investigated ultrastructurally. The early fetal period starts immediately after gonadal differentiation, approximately 27 days postcoitum (p.c.), and finishes at about 60 days postcoitum. Dates of observation were 35, 52, and 62 days p.c. At 42 days p.c. some animals were decapitated. Leydig cells at 35 days p.c. are characterized by an oval nucleus, vesicular or branched tubular smooth endoplasmic reticulum (SER), and a small quantity of rough endoplasmic reticulum (RER). The RER has two forms: a short and a long profile. The latter is closely coupled with mitochondria. The mitochondria mostly have tubular cristae. From 52 days p.c. onward the degree of coupling lessens, and it vanishes at 62 days p.c. At 52 and 62 days p.c. a very large amount of 10 nm filaments and a slight decrease in SER can be observed. The SER now has a branched tubular form, and the presence of polygonal dense bodies is also characteristic. Decapitation does not disturb normal development of the Leydig cells in the observation period. No obvious differences from controls can be observed.

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Electron Microscopic Study on the Testicular Interstitial Cells in the Mouse

Cited by 28 publications

References 44 publications

Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

Desert hedgehog (Dhh) Gene Is Required in the Mouse Testis for Formation of Adult-Type Leydig Cells and Normal Development of Peritubular Cells and Seminiferous Tubules

Leydig cell development of pig testis in the early fetal period: An ultrastructural study

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