Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures

Sirinarumitr, Theerapol; Kluge, John P.; Paul, P. S.

doi:10.1007/s007050050477

Cited by 24 publications

(23 citation statements)

References 39 publications

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“…Both processes have been previously described in FIPV-infected cats (Haagmans et al, 1996). Recent studies have shown apoptosis is a common feature in the pathogenesis of infection for several Coronaviridae members (An et al, 1999;Belyavsky et al, 1998;Liu et al, 2001;Sirinarumitr et al, 1998). FIPV, in particular, induces lymphocyte apoptosis predominantly in the CD3+ T-cell population (Haagmans et al, 1996).…”

Section: Tissue Distribution Of Fipv Rna and Histologic Lesionsmentioning

confidence: 74%

In vivo cytokine response to experimental feline infectious peritonitis virus infection

Dean

Olivry

Stanton

et al. 2003

Veterinary Microbiology

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Feline infectious peritonitis virus (FIPV) is a coronavirus that causes sporadic fatal disease in cats characterized by vasculitis, granulomatous inflammation and effusive pleuritis/peritonitis. Histologic changes in lymphoid tissues include lymphoid hyperplasia, lymphoid depletion, histiocytosis, and granuloma formation. Although viremia occurs, histologic lesions are not found uniformly throughout lymphoid tissues. We used experimental infection of cats with a highly pathogenic FIPV isolate, UCD8, to study histologic lesions, virus replication, and cytokine expression in multiple lymphoid tissues during the effusive phase of disease. Viral RNA was found in 76% of central tissues (mediastinal lymph node, spleen, mesenteric lymph node) examined, as compared to 27% of peripheral tissues (popliteal lymph node, cervical lymph node, femoral bone marrow). All tissues positive for virus replication also demonstrated lymphoid depletion. Generally, affected tissues had lower levels of IL-4 and IL-12-p40 mRNA and higher levels of IL-10 mRNA. Although no differences in IFN-gamma or TNF-alpha mRNA were measured, TNF-alpha protein expression was greater in affected tissues and demonstrated a shift in the source of TNF-alpha from macrophages to lymphocytes. Together, these results colocalize FIPV replication, lymphocyte depletion in tissues, and alterations in cytokine transcription and translation. A possible role for TNF-alpha in the previously described FIPV-induced lymphocyte apoptosis is also suggested.

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Section: Tissue Distribution Of Fipv Rna and Histologic Lesionsmentioning

confidence: 74%

In vivo cytokine response to experimental feline infectious peritonitis virus infection

Dean

Olivry

Stanton

et al. 2003

Veterinary Microbiology

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“…Apoptosis is a predominant component for contributing to the pathogenesis process of animal virus infectious diseases (Chen et al, 2008;Everett and McFadden, 1999;Shih et al, 2004). Previous studies have reported that apoptosis occurs in response to TGEV infection in ST cells and human rectal tumor adenocarcinoma cell line HRT18 modified to express the porcine aminopeptidase N (pAPN, a cellular receptor for porcine coronaviruses) in vitro (Eleouet et al, 1998(Eleouet et al, , 2000Kim et al, 2000;Sirinarumitr et al, 1998). In the present study, we detected the effects of TGEV infection in PK-15 cells and analyzed possible mechanisms involved in apoptosis of TGEV-infected cells.…”

Section: Discussionmentioning

confidence: 99%

“…In consistent with in vivo pathologic changes, TGEV also induces cytopathic effects (CPE) when propagated in vitro cultured cells (Eleouet et al, 1998;Solorzano et al, 1978). Further studies have demonstrated that TGEV infection caused CPE in swine testis (ST) cells by induction of apoptosis (Eleouet et al, 1998;Kim et al, 2000;Sirinarumitr et al, 1998). However, the exact molecular pathways of TGEV-induced apoptosis are not clear.…”

Section: Introductionmentioning

confidence: 99%

Transmissible gastroenteritis virus infection induces apoptosis through FasL- and mitochondria-mediated pathways

Ding

Huang

et al. 2012

Veterinary Microbiology

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Transmissible gastroenteritis virus (TGEV) has been reported to induce apoptosis in swine testis (ST) cells. However, the mechanisms underlying TGEV-induced apoptosis are still unclear. In this study we observed that TGEV infection induced apoptosis in porcine kidney (PK-15) cells in a time- and dose-dependent manner. TGEV infection up-regulated FasL, activated FasL-mediated apoptotic pathway, leading to activation of caspase-8 and cleavage of Bid. In addition, TGEV infection down-regulated Bcl-2, up-regulated Bax expression, promoted translocation of Bax to mitochondria, activated mitochondria-mediated apoptotic pathway, which in turn caused the release of cytochrome c and the activation of caspase-9. Both extrinsic and intrinsic pathways activated downstream effector caspase-3, followed by the cleavage of PARP, resulting in cell apoptosis. Moreover, TGEV infection did not induce significant DNA fragmentation in ammonium chloride (NH(4)Cl) pretreated PK-15 cells or cells infected with UV-inactivated TGEV. In turn, block of caspases activation also did not affect TGEV replication. Taken together, this study demonstrates that TGEV-induced apoptosis is dependent on viral replication in PK-15 cells and occurs through activation of FasL- and mitochondria-mediated apoptotic pathways.

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“…When healthy pigs are exposed to air, drinking water, feed, utensils, etc. contaminated by viruses, TGEV easily enters the piglets from the outside through the respiratory or digestive tract, eventually reaches the small intestine of the target organ, and proliferates in the intestinal epithelial cells, causing the small intestine villi to shrink or even fall off [8].…”

mentioning

confidence: 99%

Establishment of method for dual simultaneous detection of PEDV and TGEV by combination of magnetic micro-particles and nanoparticles

Luo

Chen

et al. 2020

Journal of Infection and Chemotherapy

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a b s t r a c tTransmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are the main pathogens causing viral diarrhea in pig, mixed infections of these two viruses are very common in intensive pig rearing. However, there is a lack of a method to simultaneously detect and distinguish PEDV and TGEV in preclinical levels. In this study, we aimed to establish a dual ultrasensitive nanoparticle DNA probe-based PCR assay (dual UNDP-PCR) based on functionalized magnetic bead enrichment and specific nano-technology amplification for simultaneous detection and distinguish diagnosis of PEDV and TGEV. The detection limit of dual UNDP-PCR for single or multiple infections of PEDV and TGEV is 25 copies/g, which is 400 times more sensitive than the currently known duplex RT-PCR, showing better specificity and sensitivity without cross-reaction with other viruses. For pre-clinical fecal samples, the dual UNDP-PCR showed a markedly higher positive detection rate (52.08%) than conventional duplex RT-PCR (13.21%), can rapidly and accurately identify targeted pathogens whenever simple virus infection or co-infection. In summary, this study provides a technique for detecting and distinguishing PEDV and TGEV in preclinical levels, which is high sensitivity, specificity, repeatability, low cost and broad application prospect.

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Transmissible gastroenteritis virus induced apoptosis in swine testes cell cultures

Cited by 24 publications

References 39 publications

In vivo cytokine response to experimental feline infectious peritonitis virus infection

In vivo cytokine response to experimental feline infectious peritonitis virus infection

Transmissible gastroenteritis virus infection induces apoptosis through FasL- and mitochondria-mediated pathways

Establishment of method for dual simultaneous detection of PEDV and TGEV by combination of magnetic micro-particles and nanoparticles

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