Establishment of method for dual simultaneous detection of PEDV and TGEV by combination of magnetic micro-particles and nanoparticles

Luo, Le; Chen, Jing; Li, Xiaomin; Qiao, Dan; Wang, Zhenyu; Wu, Xingchen; Du, Qian; Tong, Dewen; Huang, Yong

doi:10.1016/j.jiac.2020.01.008

Cited by 8 publications

(6 citation statements)

References 14 publications

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“…In addition, the detection rate of TGEV has been at a low level without a trend of large-scale epidemics, while the detection rate of PoRVA and PDCoV have had a rising tendency in recent years. The detection results of co-infection revealed that PEDV and PoRVA co-infection is currently the most important form of co-infection rather than the previous PEDV and TGEV co-infection [ 35 , 36 ], indicating that the monitoring and prevention of PoRVA should be strengthened. The seasonality for the prevalence of PEDV has been gradually decreasing [ 37 , 38 ], and many provinces showed high incidence throughout the year, suggesting that the prevention and control of the epidemic should be strengthened in the summer and autumn when PEDV was low in the past.…”

Section: Discussionmentioning

confidence: 99%

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Geng

et al. 2022

Viruses

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Swine enteric viruses are a major cause of piglet diarrhea, causing a devastating impact on the pork industry. To further understand the molecular epidemiology and evolutionary diversity of swine enteric viruses, we carried out a molecular epidemiological investigation of swine enteric viruses (PEDV, PDCoV, PoRVA, and TGEV) on 7107 samples collected from pig farms in south-central China. The results demonstrated that PEDV is the predominant pathogen causing piglet diarrhea, and its infection occurs mainly in relatively cold winter and spring in Hunan and Hubei provinces. The positive rate of PEDV showed an abnormal increase from 2020 to 2021, and that of PoRVA and PDCoV exhibited gradual increases from 2018 to 2021. PEDV-PoRVA and PEDV-PDCoV were the dominant co-infection modes. A genetic evolution analysis based on the PEDV S1 gene and ORF3 gene revealed that the PEDV GII-a is currently epidemic genotype, and the ORF3 gene of DY2020 belongs to a different clade relative to other GII-a strains isolated in this study. Overall, our results indicated that the variant PEDV GII-a is the main pathogen of piglet diarrhea with a trend of outbreak. G9 is the dominant PoRVA genotype and has the possibility of outbreak as well. It is therefore critical to strengthen the surveillance of PEDV and PoRVA, and to provide technical reserves for the prevention and control of piglet diarrhea.

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Section: Discussionmentioning

confidence: 99%

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Geng

et al. 2022

Viruses

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“…In addition, owing to the indistinguishable clinical symptoms from the pathological and epidemiological changes caused by porcine enteric coronaviruses, such as the closely related coronaviruses (Luo et al 2020 ; Malbec et al 2020 ; Saif et al 2019 ), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV), rapid and accurate diagnostic tools are very important for the prevention and control of the spread of PEDV in pigs.…”

Section: Introductionmentioning

confidence: 99%

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Zou

et al. 2021

Animal Diseases

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Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease. In this study, a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label. Under optimal conditions, the newly developed latex bead-based lateral flow immunoassay (LBs-LFIA) attained a limit of detection (LOD) as low as 103.60 TCID50/mL and no cross-reactivity with other related swine viruses. To solve swine feces impurity interference, by adding a filtration unit design of LFIA without an additional pretreatment procedure, the LBs-LFIA gave good agreement (92.59%) with RT-PCR results in the analysis of clinical swine fecal samples (n = 108), which was more accurate than previously reported colloidal gold LFIA (74.07%) and fluorescent LFIA (86.67%). Moreover, LBs-LFIA showed sufficient accuracy (coefficient of variance [CV] < 15%) and stable (room temperature storage life > 56 days) performance for PEDV detection, which is promising for on-site analysis and user-driven testing in pig production system.

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“…TGEV is a porcine α-CoV that is often accompanied by mixed infection with PEDV, which seriously concerns the pig industry, and there is no effective therapy yet [ 40 , 41 ]. In order to explore whether hypericin could be a good therapy candidate for both viruses, we analyzed the available crystal structure of TGEV 3CLpro (PDB: 2AMP) and aligned it with PEDV.…”

Section: Resultsmentioning

confidence: 99%

“…We found these 3CLpro sequences were highly conserved and shared more than 60% identity with PEDV 3CLpro (Table S1), especially the residues in solution 1 (Met6, Ala122, Ile151, Asn152, and Gln295) and solution 2 (Asn141, Leu164, Glu165, Asp186, Gln187, and Gln191) (Figures 3 and 5A), suggesting that hypericin might also be able to inhibit other α-CoV 3CLpro. TGEV is a porcine α-CoV that is often accompanied by mixed infection with PEDV, which seriously concerns the pig industry, and there is no effective therapy yet [40,41]. In order to explore whether hypericin could be a good therapy candidate for both viruses, we analyzed the available crystal structure of TGEV 3CLpro (PDB: 2AMP) and aligned it with PEDV.…”

Section: The Hypericin-3clpro Binding Domains Are Highly Conserved In α-Covmentioning

confidence: 99%

Hypericin Inhibit Alpha-Coronavirus Replication by Targeting 3CL Protease

Zhang

Chen

Zou

et al. 2021

Viruses

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The porcine epidemic diarrhea virus (PEDV) is an Alphacoronavirus (α-CoV) that causes high mortality in infected piglets, resulting in serious economic losses in the farming industry. Hypericin is a dianthrone compound that has been shown as an antiviral activity on several viruses. Here, we first evaluated the antiviral effect of hypericin in PEDV and found the viral replication and egression were significantly reduced with hypericin post-treatment. As hypericin has been shown in SARS-CoV-2 that it is bound to viral 3CLpro, we thus established a molecular docking between hypericin and PEDV 3CLpro using different software and found hypericin bound to 3CLpro through two pockets. These binding pockets were further verified by another docking between hypericin and PEDV 3CLpro pocket mutants, and the fluorescence resonance energy transfer (FRET) assay confirmed that hypericin inhibits the PEDV 3CLpro activity. Moreover, the alignments of α-CoV 3CLpro sequences or crystal structure revealed that the pockets mediating hypericin and PEDV 3CLpro binding were highly conserved, especially in transmissible gastroenteritis virus (TGEV). We then validated the anti-TGEV effect of hypericin through viral replication and egression. Overall, our results push forward that hypericin was for the first time shown to have an inhibitory effect on PEDV and TGEV by targeting 3CLpro, and it deserves further attention as not only a pan-anti-α-CoV compound but potentially also as a compound of other coronaviral infections.

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Establishment of method for dual simultaneous detection of PEDV and TGEV by combination of magnetic micro-particles and nanoparticles

Cited by 8 publications

References 14 publications

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design

Hypericin Inhibit Alpha-Coronavirus Replication by Targeting 3CL Protease

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