Transmissibility of viral double-stranded RNA between strains of the violet root rot fungus Helicobasidium mompa and the potential for viral dsRNA infection to this fungus using monokaryotic strains

Suzaki, Koichi; Kanematsu, Satoko; Yoshida, Kouji; Sasaki, Atsuko; Matsumoto, Naoyuki

doi:10.1007/s10267-003-0096-1

Cited by 5 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous report (Suzaki et al 2003) (Table 2). Although the two monokaryotic strains were thought to be the same or closely related (Suzaki et al 2003), HmTV1-17 virus seemed to be transmitted from these monokaryotic strains into different MCGs randomly (Table 2).…”

Section: Discussionmentioning

confidence: 86%

“…Although the two monokaryotic strains were thought to be the same or closely related (Suzaki et al 2003), HmTV1-17 virus seemed to be transmitted from these monokaryotic strains into different MCGs randomly (Table 2). Aimi et al (2001) reported that the karyotype of H. mompa was variable and that monokaryotic strains of H. mompa resulted from nuclear fusion in dikaryotic strains.…”

Section: Discussionmentioning

confidence: 96%

“…Twelve virulent isolates were randomly chosen from our fungal collection as recipients of HmTV1-17 virus. Monokaryotic strains V1M and V3M were isolated during successive culture on plate media from their parental dikaryotic strains and were found to be genetically the same or closely related by random amplified polymorphic DNA (RAPD) analysis (Suzaki et al 2003). The donor and all of the recipients of HmTV1-17 virus are dikaryotic.…”

Section: Fungal Isolatesmentioning

confidence: 99%

“…Viral dsRNA elements were isolated from 0.5 g of lyophilized mycelia according to the method described by Morris and Dodds (1979) and purified according to our previous report (Suzaki et al 2003).…”

Section: Viral Dsrna Isolationmentioning

confidence: 99%

“…Because protoplast preparation of H. mompa is difficult and hyphal anastomosis occurs between arbitrary isolates of this fungus, we thought that hyphal anastomosis for mycovirus transmission might be the only practical method to transmit a mycovirus into various isolates of H. mompa. In our previous report (Suzaki et al 2003), we showed that viral dsRNA elements in H. mompa isolate V70 containing partitivirus (Osaki et al 2002) were once transmitted into monokaryotic strains of H. mompa, and then the viral dsRNA elements were transmissible from monokaryotic strains into various MCGs, regardless of mycelial incompatibility.…”

Section: Introductionmentioning

confidence: 98%

See 4 more Smart Citations

Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

et al. 2005

Self Cite

View full text Add to dashboard Cite

Monokaryotic strains of Helicobasidium mompa were used as vectors of a mycovirus between various H. mompa isolates to examine the transmissibility of one of the mycoviruses, totivirus (HmTV1-17 virus) in the hypovirulent isolate V17 of H. mompa. The isolates that acquired HmTV1-17 virus were also examined for any alteration in the virulence. Twelve dikaryotic isolates of H. mompa, belonging to 11 mycelial compatibility groups (MCGs) and being mycelially incompatible with isolate V17, were used as recipients of HmTV1-17 virus. Two monokaryotic isolates that were mycelially incompatible with isolate V17 and all of the recipients were also used as vectors of HmTV1-17 virus between isolate V17 and the recipients. When isolate V17 and recipients were directly paired on plate media, HmTV1-17 virus was transmitted from isolate V17 into 2 of the 12 recipients (i.e., 2 of the 11 MCGs). Two monokaryotic strains were paired with isolate V17, and the monokaryotic strains that acquired HmTV1-17 virus were then used as new virus donors. When the monokaryotic strains containing HmTV1-17 virus were paired with the 12 recipients, HmTV1-17 virus was transmitted into 7 of the 12 recipients from the monokaryotic strains (i.e., 7 of 11 MCGs). Based on these results, we concluded that monokaryotic strains could act as vectors to transmit HmTV1-17 virus into H. mompa isolates. When four of the H. mompa isolates that acquired HmTV1-17 virus were used to inoculate apple rootstock Malus prunifolia, the virulence of all of the isolates was attenuated from that of their parental isolates. Moreover, because the DNA fingerprints of the fungal isolates that acquired HmTV1-17 virus were the same as those of their parental isolates, the infection with HmTV1-17 virus is considered the cause of virulence attenuation of H. mompa.

show abstract

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Section: Fungal Isolatesmentioning

confidence: 99%

Section: Viral Dsrna Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

Hypovirulent strain of the violet root rot fungus Helicobasidium mompa

Ikeda

Nakamura

Matsumoto

2003

Journal of General Plant Pathology

Self Cite

View full text Add to dashboard Cite

The hyphal tip was isolated from 13 weakly or moderately virulent strains of Helicobasidium mompa to remove double-stranded (ds) RNAs and demonstrate their role as the hypovirulence factor. All of 829 hyphal tip subcultures retained dsRNAs. However, strain v670 containing two large fragments (Ͼ10 kb) and one small fragment (ca. 2.3 kb) of dsRNA lost the largest fragment in 3 of 63 subcultures analyzed. One of the three subcultures (v670hti) was used to inoculate carrots to regain virulence compared to the parental strain v670. When isolate v670hti was paired with v670, the largest fragment was reintroduced to v670hti, and its virulence was diminished. Northern blot analysis with two probes hybridizing dsRNA fragments in most H. mompa strains revealed that the largest fragment involved in hypovirulence was different from two other fragments that are common in Japan. These results indicate that the largest dsRNA fragment in strain v670 is associated with hypovirulence in H. mompa.

show abstract

Transmission of the M2 double-stranded RNA inRhizoctonia solanianastomosis group 3 (AG-3)

Charlton¹,

Cubeta

2007

Mycologia

View full text Add to dashboard Cite

Horizontal transmission of the 3.57 kb M2 double-stranded RNA (dsRNA) between mycelia of somatically incompatible isolates of Rhizoctonia solani anastomosis group 3 (AG-3), an economically important pathogen of cultivated plants in the family Solanaceae, was investigated. Nine donor isolates of R. solani AG-3 containing the M2 dsRNA were paired on potato-dextrose agar with each of three different recipient isolates where the M2 dsRNA was absent. Reverse-transcription PCR (RT-PCR) was used to detect horizontal transmission of the M2 dsRNA via hyphal anastomosis from donor to recipient isolates by examining hyphal explants taken 3 cm from the hyphal interaction zone. PCR-RFLP genetic-based markers of two nuclear loci and one mitochondrial locus were used to confirm identity and transmission between donor and recipient isolates of R. solani AG-3. The frequency of transmission observed between 72 pairings of the eight donor and three recipient isolates was approximately 4% of the total pairings, and differences in the phenotype of the recipient isolates after acquisition of the M2 dsRNA via horizontal transmission were observed. To our knowledge this represents the first demonstration of transmission of dsRNA between genetically different individuals of R. solani confirmed with nuclear and mitochondrial markers. These results suggest that transmission can occur between somatically incompatible isolates of R. solani AG-3 but that maintenance of the dsRNA in the recipient isolates was not stable after repeated subculturing on nutrient medium.

show abstract

Transmissibility of viral double-stranded RNA between strains of the violet root rot fungus Helicobasidium mompa and the potential for viral dsRNA infection to this fungus using monokaryotic strains

Cited by 5 publications

References 23 publications

Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

Horizontal transmission and host-virulence attenuation of totivirus in violet root rot fungus Helicobasidium mompa

Hypovirulent strain of the violet root rot fungus Helicobasidium mompa

Transmission of the M2 double-stranded RNA inRhizoctonia solanianastomosis group 3 (AG-3)

Contact Info

Product

Resources

About