Translocation of Calcium in the Bean Plant.

Biddulph, Orlin; Cory, R.; Biddulph, Susann

doi:10.1104/pp.34.5.512

Cited by 100 publications

(53 citation statements)

References 3 publications

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“…Petrie (1934) with tomatoes and apples; Biddulph et al (1958), Bukovac and Wittwer (1961), and Kiselev (1961) with beans; Rinnie and Langston (1960) with peppermint; Peterburgskii (1962) with oats, peas, and potatoes; and Norton and Wittwer (1963) with strawberry].…”

Section: Introductionmentioning

confidence: 99%

“…Thus translocation was obtained by dehydrating the plants (Biddulph, Cory, and Biddulph 1959;Taylor, Moore, and Drinkwater 1961), by treatment with diethyl ether (Bukovac, Wittwer, and Tukey 1956;Biddulph, Cory, and Biddulph 1959), and in some instances by treatment with 2,3,5-triiodobenzoic acid (Kessler and Moscicki 1958;Bukovac and Wittwer 1961). Taylor, Moore, and Drinkwater (1961) were unsuccessful when they used the latter compound as a translocating agent.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Chelation and of Certain Cations on the Mobility of Foliar-Applied 45ca in Stock, Broad Bean, Peas, and Subterranean Clover

Millikan¹,

Hanger²

1965

Aust. Jnl. Of Bio. Sci.

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SummaryA series of water-culture experiments was conducted with broad bean (Vicia faba L.), common stock (Mathiola incana R. Br.), subterranean clover (Trifolium Bubterraneum L.), and garden peas (PiBum Bativum L.) grown in normal-and lowcalcium nutrient solutions. Leaves of the plants were either injected or s,,!rfacetreated with an aqueous solution of 45CaCh with and without various additions to the dose. The subsequent distribution of 45Ca in the plants was determined by means of radioautographs and radioassays.In each species, where nothing was added to the dose, there was negligible movement of 45Ca from the treated leaf. With broad bean or common stock or both, the addition of small amounts of EDTA (disodium salt) or HCI or both, or of citric acid, caused marked movement of 45Ca in an acropetal direction in both the normal and calcium-deficient plants. Marked accumulation of 45Ca occurred in the epidermal hairs and anthers of common stock. There was also some evidence of retranslocation of 45Ca in this species.A series of experiments with subterranean clover confirmed the effect of EDTA in promoting mobility of 45Ca when either injected into a leaf or applied to the uninjured leaf surface. With low concentrations of EDTA, movement was mainly acropetal, but with a high concentration marked basipetal movement to the roots also occurred.When graduated amounts of non-radioactive calcium (as CaCh.6H20) or equivalent amounts of magnesium (as MgCh.6H20) were added to the dose, increasing mobility of the injected 45Ca occurred in both normal and calcium-deficient plants. With the highest levels of calcium or magnesium additions, movement of 45Ca occurred throughout the plant including the cotyledons and the whole of the root system.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Chelation and of Certain Cations on the Mobility of Foliar-Applied 45ca in Stock, Broad Bean, Peas, and Subterranean Clover

Millikan¹,

Hanger²

1965

Aust. Jnl. Of Bio. Sci.

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“…Langston (1956), who dried tomato and peppermint plants for 24 hr before autoradiography, found the greatest concentration of 45Ca in leaf veins, petioles, and stem tissues. Later Biddulph, Cory, and Biddulph (1959) observed that 45Ca moved from bean leaves via the xylem during drying.…”

Section: Redistribution Of 45c3 In T Subterraneum and A Majus 1125mentioning

confidence: 99%

Redistribution of 45ca in Trifolium Subterranevm L. and Antirrhinum Majus L.

Millikan¹,

Hanger²

1967

Aust. Jnl. Of Bio. Sci.

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SummaryInjection of non·radioactive calcium into leaf 3 of normal· or low-calcium T. 8ubterraneum plants immediately after transfer to tracer-free solutions caused changes in the distribution of 450a in the low-calcium plants only. In these plants the mean 450a concentration in the laminae of all leaves was appreciably increased as a result of the influx of 450a from the petioles.For both normal-and low-calcium plants 2 weeks growth in tracer-free solutions resulted in a reduction of the mean 450a concentration in both laminae and petioles of the old leaves, while the isotope appeared in riew leaves produced during this period.A calcium injection at the end of this growth period caused a further reduction in mean 450a concentration in laminae and petioles of the old leaves, and movement of the isotope into the new leaves. This movement was mainly into the lamina centre of the low-calcium and, to a much lesser degree, into the petioles of the normal-calcium leaves.Dialysis of 450a in leaf 2 showed that these changes in ooncentration of the isotope were restricted to the water-soluble fraction in the normal-calcium samples. In the low-calcium samples most of the change also occurred in the water-soluble fraction, but small changes were also recorded in the fractions extracted by sodium chloride, magnesium chloride, and hydrochloric acid.With A. majus plants a much greater redistribution of previously deposited 450a occurred in both original and new leaves of low-calcium plants transferred to tracer-free, normal-calcium solutions than in plants transferred to low-calcium solutions.Redistribution of 450a occurred also in normal-calcium plants transferred to normal-calcium solutions and to a lesser extent in normal plants transferred to low-calcium solutions. This redistribution of 450a continued between days 8 and 16 after transfer to tracer-free solutions. 450a artefacts were produced in autoradiographs by drying A. majus plants grown in normal but not in low-calcium nutrient solutions.

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“…Strontium also behaves in the same way (2). There are some conditions, however, under which calcium does become mobile in plant tissue (3). For example, during the germination of the bean seed, calcium present in the cotyledons becomes distributed throughout the root, hypocotyl, and primary leaves.…”

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confidence: 99%

Stronium Mobility in Germinating Seeds and Plants

Creger

Allen

1969

Plant Physiol.

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Abstract. The uptake of strontium in the bean plant (Phaseolus vulgaris) was linear for the first 34 hr during continuous exposure to radiostrontium. After 35 hr there was a sharp increase in the rate of uptake to 48 hr. Radioactivity could be detected in the plant as early as 1 hr after addition of radiostrontium to the growth medium.Seventy-five percent of the radiostrontium was located in the seed coat immediately after soaking bean seed in an 59Sr solution. This radioactivity in the seed coat decreased rapidly up to the tenth day, while the proportion of stkontium in the cotyledons increased, after which time the proportion of radiostrontium in the cotyledons began to decrease. The amount of radioactivity of the seedling axis increased constantly, as did the activity of the first and second leaves after their appearance on about the seventh day.The radioactivity in the developing third and fourth leaves of ootton plants (Gossypium hirsutum) increased at the expense of the radioactivity in the first 2 leaves and stems. This represents a movement into certain parts and then a retranslocation out of these parts as other tissue begins to develop.Sources of radioactivity whiclh represent potential internal radiation hazards to animals include fallout following explosions of atomic weapons, or industrial accidents resulting in contamination of feed, plant life or water which is destined for animal consumption.Since strontium meets with no great difficultv in gaining entrance into the plant, the question of transport within the plant becomes important. When once delivered to a particular organ in the plant, calcium becomes largely immobile (1). Strontium also behaves in the same way (2). There are some conditions, however, under which calcium does become mobile in plant tissue (3). For example, during the germination of the bean seed, calcium present in the cotyledons becomes distributed throughout the root, hypocotyl, and primary leaves. During the growth of tulip bulbs, calcium moves from the bulb into the growing leaves (4). The (6). and these data were calculated by the computer method of Creger et al. (7), which extrapolates activity 'to the time of original application of the isotope to the plant.For the first phase of this investigation, the objective was to determine the rate of uptake of strontium bv bean seedlings. Bean seeds were germinated in paper towels and allowed to grow in a liquid culture solution until 10 days old. The plants were then transferred to 10 ml of identical culture solution that contained 1 /Ic 89Sr per ml (no cold Sr was added). At certain time intervals plants were removed, washed twice with 1 % EDTA and once with deionized water, separated into tops and roots, and analyzed for radiostrontium. Five plants were analyzed at each interval up to 48 hr.For the second phase of this investigationi, bean seeds were soaked for 24 hr in a culture solution containing 10 Mc 89Sr per ml. The seeds were then removed from the solution, washed twice with I % EDTA and once with deionized water an...

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Translocation of Calcium in the Bean Plant.

Cited by 100 publications

References 3 publications

Effects of Chelation and of Certain Cations on the Mobility of Foliar-Applied 45ca in Stock, Broad Bean, Peas, and Subterranean Clover

Effects of Chelation and of Certain Cations on the Mobility of Foliar-Applied 45ca in Stock, Broad Bean, Peas, and Subterranean Clover

Redistribution of 45ca in Trifolium Subterranevm L. and Antirrhinum Majus L.

Stronium Mobility in Germinating Seeds and Plants

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