Translational diffusion of a transient radical studied by the transient grating method, pyrazinyl radical in 2-propanol

Terazima, Masahide; Hirota, Noboru

doi:10.1063/1.464819

Cited by 101 publications

(121 citation statements)

References 27 publications

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“…If reaction kinetics are negligible, the time profile is expressed by a combination of the terms of exp(− Dq 2 t) so that the signals plotted against q 2 t should be identical. 23 However, the observed profiles are strongly dependent on q 2 , especially for the signals at larger q 2 -values. Thus, the TG signals were analyzed based on the timedependent D model as follows.…”

Section: Resultsmentioning

confidence: 95%

“…6,[23][24][25][26] The time profile represents the reaction kinetics as well as the thermal diffusion and molecular diffusion processes. Figure 1 shows a typical TG signal after photoexcitation of PixD in the buffer solution at a grating wavenumber q 2 = 4.0 × 10 11 m − 2 and a laser power of 0.2 mJ cm − 2 .…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the faster decay signal was attributed to the thermal grating component due to the heat release from the excited state of the flavin chromophore. The temporal profile of the thermal grating is expressed by: 23,24 …”

Section: Resultsmentioning

confidence: 99%

“…6,[20][21][22][23]25 Usually, 20-100 signals were averaged by a digital oscilloscope (TDS-7104; Tektronix) to improve the signal-to-noise ratio. The repetition rate of the excitation was b 0.03 Hz to avoid photoexcitation of a photoproduct.…”

Section: Transient Gratingmentioning

confidence: 99%

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Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694)

Tanaka

Nakasone

Okajima

et al. 2011

Journal of Molecular Biology

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The light-induced reaction of the BLUF (blue light photoreceptor using flavin adenine dinucleotide) photoreceptor PixD from Synechocystis sp. PCC6803 (Slr1694) was investigated using the time-resolved transient grating method. A conformational change coupled with a volume contraction of 13 mL mol(-1) was observed with a time constant of 45 ms following photoexcitation. At a weak excitation light intensity, there were no further changes in volume and diffusion coefficient (D). The determined D-value (3.7×10(-11) m(2) s(-1)) suggests that PixD exists as a decamer in solution, and this oligomeric state was confirmed by size-exclusion chromatography and blue native polyacrylamide gel electrophoresis. Surprisingly, by increasing the excitation laser power, we observed a large increase in D with a time constant of 350 ms following the volume contraction reaction. The D-value of this photoproduct species (7.5×10(-11) m(2) s(-1)) is close to that of the PixD dimer. Combined with transient grating and size-exclusion chromatography measurements under light-illuminated conditions, the light-induced increase in D was attributed to a transient dissociation reaction of the PixD decamer to a dimer. For the M93A-mutated PixD, no volume or D-change was observed. Furthermore, we showed that the M93A mutant did not form the decamer but only the dimer in the dark state. These results indicate that the formation of the decamer and the conformational change around the Met residue are important factors that control the regulation of the downstream signal transduction by the PixD photoreceptor.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Transient Gratingmentioning

confidence: 99%

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Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694)

Tanaka

Nakasone

Okajima

et al. 2011

Journal of Molecular Biology

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“…Although dynamic light scattering (Lehermayr et al, 2011), the Taylor-dispersion method (Chamieh et al, 2016, Hogstedt et al, 2016, and Oukacine et al, 2015, and nuclear-magnetic-resonance spectroscopy (Latour et al, 1994) are widely used as methods for measuring the diffusion coefficient, a long measurement time is required. A transient-grating method (Terazima et al, 1993) and fluorescence-correlation spectroscopy (Magde et al, 1972, Ruan et al, 2004, and Dertinger et al, 2007 have realized high-speed and small-sample-volume diffusion-coefficient sensing; however, these methods require surface modification or the application of additives to the sample in advance.…”

Section: Introductionmentioning

confidence: 99%

Decay time control of mass diffusion in a transient grating using a fringe-tunable electrothermal Fresnel mirror

Kiuchi

Taguchi

Nagasaka

2017

JTST

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Application of the Transient Grating Method to the Measurement of Transport Properties for High Pressure Fluids

Kimura

Kanda

Terazima

et al. 1995

Ber Bunsenges Phys Chem

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We have applied the transient grating method to the measurement of the transport properties for high pressure fluids, such as carbon dioxide and trifluoromethane from the gaseous-to the liquid-density regions for the first time. Using nitrobenzene and methyl red as probe moolecules, we have made the transient grating in a high pressure fluid by a nanosecond-pulsed laser. By the analysis of the timc profile ol' the transient grating signal in sub-microsecond to sub-millisecond range, we have succeeded in measuring the density and the temperature dependences of various transport properties such as the thermal diffusion constant, the sound velocity, the sound absorption, and the mass diffusion constant under the same experimental condition. The thermal diffusion constant of our mcasurcnient is identical with 4% error to the value reported in literature, irrespective of probe moleculcs. We have determined the sound velocity within 0.5% error in a high frequency region around hundreds of MHz. The mass diffusion constant of methyl red is determined within 5% error in trifluoromethane at high densities. We have also demonstrated that the thermal diffusion obeys the critical exponent near the critical point. Ncr. Bun.sw,yn. Plij)s. C'hem. 99, 196 ~ 203 (1995) No. 2 0 VCII VerlaRs~eselbchuft mDH. D-694JI Weinheirn, I995 0005-9021/95/

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Translational diffusion of a transient radical studied by the transient grating method, pyrazinyl radical in 2-propanol

Abstract: Articles you may be interested inStudy of the translational diffusion of the benzophenone ketyl radical in comparison with stable molecules in room temperature ionic liquids by transient grating spectroscopy

Cited by 101 publications

References 27 publications

Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694)

Light-Induced Conformational Change and Transient Dissociation Reaction of the BLUF Photoreceptor Synechocystis PixD (Slr1694)

Decay time control of mass diffusion in a transient grating using a fringe-tunable electrothermal Fresnel mirror

Application of the Transient Grating Method to the Measurement of Transport Properties for High Pressure Fluids

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