Translational applications of molecular imaging in cardiovascular disease and stem cell therapy

Du, Wei; Tao, Hongyan; Zhao, Shihua; He, Zuo-Xiang; Li, Zongjin

doi:10.1016/j.biochi.2015.06.021

Cited by 21 publications

(15 citation statements)

References 86 publications

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“…10,41,42 BLI revealed that the survival of transplanted ADSCs could be significantly enhanced by CS-IGF-1C hydrogel application. Besides monitoring cell survival, molecular imaging also has been used for real-time visualization of VEGFR2 expressions in vivo, which provides direct evidence for disclosure of the therapeutic mechanism of angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Besides monitoring cell survival, molecular imaging also has been used for real-time visualization of VEGFR2 expressions in vivo, which provides direct evidence for disclosure of the therapeutic mechanism of angiogenesis. 42 ADSCs with CS hydrogel treatment resulted in a modest enhancement in angiogenesis after ischemia, which was improved by CS-IGF-1C peptide modification. This increase in angiogenic response may result from prolonged cytokine release by IGF-1C hydrogel application, as previously documented.…”

Section: Discussionmentioning

confidence: 99%

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IGF-1 C Domain–Modified Hydrogel Enhances Cell Therapy for AKI

Feng

Zhang

Yang³

et al. 2016

JASN

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104

135

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Low cell retention and engraftment after transplantation limit the successful application of stem cell therapy for AKI. Engineered microenvironments consisting of a hydrogel matrix and growth factors have been increasingly successful in controlling stem cell fate by mimicking native stem cell niche components. Here, we synthesized a bioactive hydrogel by immobilizing the C domain peptide of IGF-1 (IGF-1C) on chitosan, and we hypothesized that this hydrogel could provide a favorable niche for adipose-derived mesenchymal stem cells (ADSCs) and thereby enhance cell survival in an AKI model. In vitro studies demonstrated that compared with no hydrogel or chitosan hydrogel only, the chitosan-IGF-1C hydrogel increased cell viability through paracrine effects. In vivo, cotransplantation of the chitosan-IGF-1C hydrogel and ADSCs in ischemic kidneys ameliorated renal function, likely by the observed promotion of stem cell survival and angiogenesis, as visualized by bioluminescence imaging and attenuation of fibrosis. In conclusion, IGF-1C immobilized on a chitosan hydrogel provides an artificial microenvironment for ADSCs and may be a promising therapeutic approach for AKI.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

IGF-1 C Domain–Modified Hydrogel Enhances Cell Therapy for AKI

Feng

Zhang

Yang³

et al. 2016

JASN

Self Cite

104

135

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“…Acute myocardial infarction (AMI) is associated with considerable morbidity and mortality worldwide (1,2). The majority of myocardial infarcts result from coronary atherosclerosis, complicated by acute rupture of an atherosclerotic plaque and subsequent formation of coronary thrombus.…”

Section: Introductionmentioning

confidence: 99%

Increased complements and high-sensitivity C-reactive protein predict heart failure in acute myocardial infarction

Liu

et al. 2016

Biomedical Reports

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The aim of the present study was to investigate whether the serum levels of complements and high-sensitivity C-reactive protein (hs-CRP) in patients with acute myocardial infarction (AMI) are associated with the severity of myocardial injury. Consecutive patients (n=110) with AMI and 33 healthy individuals, who served as control subjects, were enrolled from May 2013 to February 2015. These patients were divided into two groups, those with ST segment elevation MI (STEMI) and those with non-ST segment elevation MI (NSTEMI). The patients with STEMI exhibited progression to diastolic dysfunction and heart failure. Furthermore, the results revealed that the level of serum complement and hs-CRP in patients with AMI increased rapidly when compared with the subjects from the control group, particularly in the STEMI patients, at different time-points. A statistically significant elevation of the complement and hs-CRP levels was observed at day 3 after AMI in the STEMI group. The activation of complement and hs-CRP following AMI may serve as a specific marker to successfully predict left ventricular dysfunction. Thus, biomarker-based approaches may be adopted to identify the severity of AMI with distinct pathophysiologic responses in order to rationally implement clinical therapeutic strategies.

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“…It has been widely agreed that the most critical mechanism by which stem cells confer reparative benefits are their paracrine actions including anti-inflammatory, pro-proliferative, anti-apoptotic, and pro-angigenic effects31. Considering the therapeutic efficacy of MSCs is associated with cell engraftment efficiency, strategies aimed at prolonging cell survival with biomaterials to extend paracrine activation should be introduced for future stem cell therapy103233.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects

Cai

Shen

Song

et al. 2016

Sci Rep

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102

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Stem cells are promising for the treatment of myocardial infarction (MI) and large animal models should be used to better understand the full spectrum of stem cell actions and preclinical evidences. In this study, bone marrow mesenchymal stem cells (BM-MSCs) were transplanted into swine heart ischemia model. To detect glucose metabolism in global left ventricular myocardium and regional myocardium, combined with assessment of cardiac function, positron emission tomography-computer tomography (PET-CT) and magnetic resonance imaging (MRI) were performed. To study the changes of glucose transporters and glucose metabolism-related enzymes and the signal transduction pathway, RT-PCR, Western-blot, and immunohistochemistry were carried out. Myocardium metabolic evaluation by PET-CT showed that mean signal intensity (MSI) increased in these segments at week 4 compared with that at week 1 after BM-MSCs transplantation. Moreover, MRI demonstrated significant function enhancement in BM-MSCs group. The gene expressions of glucose transporters (GLUT1, GLUT4), glucose metabolism-related enzymes phosphofructokinase (PFK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) and 70-kDa ribosomal protein S6 kinase (p70s6k) in BM-MSCs injected areas were up-regulated at week 4 after BM-MSCs transplantation and this was confirmed by Western-blot and immunohistochemistry. In conclusions, BM-MSCs transplantation could improve cardiac function in swine MI model by activation of mTOR signal transduction pathway.

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Translational applications of molecular imaging in cardiovascular disease and stem cell therapy

Cited by 21 publications

References 86 publications

IGF-1 C Domain–Modified Hydrogel Enhances Cell Therapy for AKI

IGF-1 C Domain–Modified Hydrogel Enhances Cell Therapy for AKI

Increased complements and high-sensitivity C-reactive protein predict heart failure in acute myocardial infarction

Bone Marrow Mesenchymal Stem Cells (BM-MSCs) Improve Heart Function in Swine Myocardial Infarction Model through Paracrine Effects

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