Translation initiation factor 5A in Picrorhiza is up-regulated during leaf senescence and in response to abscisic acid

Parkash, Jai; Vaidya, Tanmay; Kirti, Shruti; Dutt, Som

doi:10.1016/j.gene.2014.03.032

Cited by 20 publications

(14 citation statements)

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“…It was proposed that different isoforms of eIF5A function distinctively in regulation of cell division and cell death. Also, eIF5A has been found to play a pivotal role in senescence (Thompson et al 2004, Parkash et al 2014). Thus, the two different types of functions raises the possibility that eIF5A isoforms are elements of a biological switch that is in one position in dividing cells and in another position in dying cells.…”

Section: Roles Of Translation Initiation System In Plantsmentioning

confidence: 99%

“…It has also been shown that transgenic plants overexpressing Xu et al 2011 eIF5A exhibit an enhanced fitness when exposed to osmotic and nutrient (N, P, and K) stresses (Ma et al 2010). In Picrorhiza kurrooa, a plant of a high altitude area of the Himalayan region, expression of eIF5a increases during leaf senescence and in response to abscisic acid thus suggesting its association with leaf senescence (Parkash et al 2014). The eIF4 has been observed to be involved in a selective translation of mRNAs related to root development during normal growth conditions in Arabidopsis (Martínez-Silva et al 2012).…”

Section: Roles Of Translation Initiation System In Plantsmentioning

confidence: 99%

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Translation initiation in plants: roles and implications beyond protein synthesis

et al. 2015

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Protein synthesis is a ubiquitous and essential process in all organisms, including plants. It is primarily regulated at translation initiation stage which is mediated through a number of translation initiation factors (eIFs). It is now becoming more apparent that in addition to synthesis of proteins, eIFs also regulate various aspects of plant development and their interaction with environment. Translation initiation factors, such as eIF3, eIF4A, eIF4E, eIF4G, and eIF5A affect different processes during vegetative and reproductive growth like embryogenesis, xylogenesis, flowering, sporogenesis, pollen germination, etc. On the contrary, eIF1A, eIF2, eIF4, and eIF5A are associated with interaction of plants with different abiotic stresses, such as high temperature, salinity, oxidative stress, etc. Similarly, eIF4E and eIF4G have roles in interaction with many viruses. Therefore, the translation initiation factors are important candidates for improving plant performance and adaptation. A large number of genes encoding eIFs can functionally be validated and utilized through genetic engineering approaches for better adaptability and performance of plants by inhibiting/minimizing or increasing expression of desired eIF(s).

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Section: Roles Of Translation Initiation System In Plantsmentioning

confidence: 99%

Section: Roles Of Translation Initiation System In Plantsmentioning

confidence: 99%

Translation initiation in plants: roles and implications beyond protein synthesis

et al. 2015

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“…Complementary DNA (cDNA) was synthesized from 2 μg of DNase treated total RNA as a template in 20 μl reaction volume by using cDNA synthesis kit (Invitrogen, USA) as described previously (Parkash et al, 2014b). Degenerate primers (Pk-cbcp-dF1, Pkcbcp-dR1) for Pk-cbcp were designed from the conserved regions of corresponding gene reported from different plant sources, and the partial gene sequence was amplified by PCR as detailed in Table 1.…”

Section: Cloning Of Cdna Of Pk-cbcpmentioning

confidence: 99%

“…To analyze the expression level of the cloned gene, total RNA was isolated from leaf tissue (100 mg) using PureLink™ RNA Mini Kit (Invitrogen, USA). The cDNA was synthesized from DNA-free RNA using SuperScript® III Reverse Transcriptase (Invitrogen, USA) as described previously (Parkash et al, 2014b). This cDNA was to be used as template for Reverse transcription-PCR reaction using gene specific primers (Pk-cbcp-expF1, Pk-cbcp-expR1) as mentioned in Table 1.…”

Section: Semi-quantitative Expression Analysis By Rt-pcrmentioning

confidence: 99%

Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

et al. 2015

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Medicinal importance of Picrorhiza (Picrorhiza kurrooa Royle ex Benth -an herb of western Himalayan region) and its endangered status in Red Data Book presses an urgent need for intensive R&D interventions towards ensuring its availability for the medicinal use, its sustainability and improvement. The present study was conducted on cathepsin B cysteine protease in Picrorhiza. Cathepsin B cysteine protease has been reported to function in diverse processes such as senescence, abscission, programmed cell death, fruit ripening and in response to pathogen and pest attacks. A full-length cDNA-Pk-cbcp encoding cathepsin B-like cysteine protease was cloned from Picrorhiza. The full length Pk-cbcp cDNA consisted of 1369 bp with an open reading frame of 1080 bp, 80 bp 5′ untranslated region and 209 bp 3′ untranslated region. The deduced Pk-cbcp protein contained 359 amino acids with a molecular weight of 39.981 kDa and an isoelectric point of 5.75. Secondary structure analysis revealed that Pk-cbcp had 28.97% α-helices, 14.48% β-turns, 19.50% extended strands and 37.05% random coils. Semi-quantitative PCR analysis revealed 157% higher expression of Pk-cbcp during senescence compared to that of pre-senescence. Further, application of phytohormones abscisic acid, jasmonic acid and cytokinin influenced the temporal expression status of Pk-cbcp. Abscisic acid and jasmonic acid increased the expression level whereas cytokinin reduced the expression. The findings suggest the role of Pk-cbcp in leaf senescence in Picrorhiza which may be differentially mediated through phytohormones.

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“…Likewise, we also found a clear correlation between decreased sugar content and increased endogenous ABA content in our sugar starvation treatments. Previous studies reported that exogenous ABA induce leaf senescence in wheat flag leaves by inducing the expression of many senescence-associated genes (Yang et al[27] [28]. The endogenous level of ABA in plant tissues was controlled by the equilibrium between ABA biosynthesis and catabolism.…”

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confidence: 99%

Participation of ABA Metabolism and ROS Generation in Sugar Starvation-Induced Senescence of Rice Flag Leaves

Mau¹,

Wang²,

Y³

et al. 2019

Preprint

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Background: Both sucrose and abscisic acid (ABA) play pivotal role in the regulation of plant leaf senescence. However, the exact mechanism by which sugar starvation , ABA, and reactive oxygen species (ROS) interact with each other during leaf senescence remains largely unknown. In this study, the genotype-dependent alteration in temporal patterns of sugar concentration during leaf senescence and its relation to ABA metabolism and ROS generation were investigated by using the premature senescence of flag leaf ( psf ) mutant and its wild type. Results: Results showed that sugar starvation-induced leaf senescence was closely associated with the endogenous ABA concentration and ROS level in senescent leaves. Sugar starvation accelerated leaf senescence, concomitantly with the marked increase in ABA concentration and malonaldehyde (MDA) accumulation in detached leaves. Conversely, exogenous sugar treatment significantly suppressed the ABA concentration ad ROS level in detached leaves, thus leaf senescence was delayed by exogenous sugar supply. Pharmacological tests revealed that ABA biosynthesis inhibitor (NDGA) delayed the sugar starvation-induced leaf senescence, while ABA catabolism inhibitor (DNCZ) accelerated leaf senescence and significantly increased the endogenous ABA content in senescent leaves. For the expression patterns of ABA synthesis and catabolism related genes induced by sugar starvation, exogenous sucrose supply, NDGA and DNCZ. sugar starvation up-regulated the OsABA8ox1 transcript, while exogenous sucrose and NDGA down-regulated the transciptional expressions of OsNCED1 , OsNCED4 and OsNCED5 and OsABA8ox2 and OsABA8ox3 e by sugar starvation and DNCZ, while the transcript of was increased. Conclusion: Together, our results demonstrated that the rise in endogenous ABA content during sugar starvation-induced leaf senescence is mostly caused by the suppression of ABA catabolism, rather than the enhancement of ABA biosynthesis, and the expression of ABA metabolic genes determines the equilibrium between ABA biosynthesis and catabolism that eventually influence cross-talk between endogenous factors. The breaking for the equilibrium between ABA biosynthesis and catabolism was strongly responsible for sugar starvation-induced leaf senescence, which was resulted from the suppression of ABA catabolism, rather than the enhancement of ABA biosynthesis .

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Translation initiation factor 5A in Picrorhiza is up-regulated during leaf senescence and in response to abscisic acid

Cited by 20 publications

References 20 publications

Translation initiation in plants: roles and implications beyond protein synthesis

Translation initiation in plants: roles and implications beyond protein synthesis

Cathepsin B cysteine protease gene is upregulated during leaf senescence and exhibits differential expression behavior in response to phytohormones in Picrorhiza kurrooa Royle ex Benth.

Participation of ABA Metabolism and ROS Generation in Sugar Starvation-Induced Senescence of Rice Flag Leaves

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