Translation and functional roles of circular RNAs in human cancer

Lei, Ming; Zheng, Guantao; Ning, Qianqian; Zheng, Junnian; Dong, Dong

doi:10.1186/s12943-020-1135-7

Cited by 491 publications

(396 citation statements)

References 108 publications

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“…[6][7][8] As a special non-coding RNA, circular RNA (circRNA) has been shown to play a vital role in the occurrence of cancers. 9,10 CircRNA is considered to be an important regulator of cancer progression and mainly plays anti-cancer or pro-cancer role. 11,12 At present, many circRNAs are found in NSCLC, and their functions are illuminated.…”

Section: Introductionmentioning

confidence: 99%

<p>Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network</p>

Li¹,

Long²,

Niu³

et al. 2020

CMAR

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Background: Hypoxia has been shown to induce the malignant progression of cancer, including non-small cell lung cancer (NSCLC). Circular RNA (circRNA) is considered to be an important regulator of cancer progression. However, the role of a newly discovered circRNA, circ_0000376, in the progression of NSCLC is unclear. Methods: The relative expression levels of circ_0000376, miR-1182 and neuro-oncological ventral antigen 2 (NOVA2) were detected via quantitative real-time polymerase chain reaction (qRT-PCR). Glucose consumption and lactate production were determined using Glucose Assay Kit and Lactate Assay Kit, respectively. Moreover, the protein levels of glycolysis markers and NOVA2 were measured using Western blot (WB) analysis. Furthermore, 3-(4, 5-dimethyl-2 thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay was performed to assess cell viability, and transwell assay was employed to evaluate cell migration and invasion. The interaction between miR-1182 and circ_0000376 or NOVA2 was confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. In addition, animal experiments were conducted to assess the influence of circ_0000376 silencing on NSCLC tumor growth in vivo. Results: Circ_0000376 was upregulated in NSCLC, and its high expression was related to the poor overall survival of NSCLC patients. Hypoxia could enhance circ_0000376 expression and promote the glycolysis, viability, migration, and invasion of NSCLC cells. However, silencing of circ_0000376 could inhibit the glycolysis, viability, migration, and invasion of hypoxia-induced NSCLC cells. Additionally, circ_0000376 could sponge miR-1182, and miR-1182 could target NOVA2. MiR-1182 silencing could reverse the inhibitory effect of circ_0000376 knockdown on NSCLC progression, and NOVA2 overexpression also could reverse the suppressive effect of miR-1182 overexpression on NSCLC progression. Meanwhile, miR-1182 inhibitor could invert the negative regulation effect of circ_0000376 silencing on NOVA2 expression. In addition, circ_0000376 knockdown inhibited the NSCLC tumor growth via regulating the miR-1182 and NOVA2 expression in vivo. Conclusion: Circ_0000376 promoted NSCLC progression by regulating the miR-1182/NOVA2 axis, suggesting that circ_0000376 might be a potential biomarker for NSCLC treatment.

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Section: Introductionmentioning

confidence: 99%

<p>Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network</p>

Li¹,

Long²,

Niu³

et al. 2020

CMAR

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show abstract

“…(b) circRNAs are widely present in eukaryotic cells and have high expression levels in cells, tissues, and body uids. (c) Compared with traditional biomarkers, circRNAs are more speci c in terms of tissue expression [36].…”

Section: Discussionmentioning

confidence: 99%

CircRNAs as Potential Biomarkers for the Clinicopathology and Prognosis of Glioma Patients:A Meta-analysis

Ding

Yang

Geng

et al. 2020

Preprint

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Abstract Background: An increasing number of studies have reported circular RNAs (circRNAs) as new potential biomarkers for the prognosis of gliomas. However, the overall prognostic value of circRNAs for glioma remains unclear. Therefore, this study is the first comprehensive evaluation of the clinicopathological and prognostic value of dysregulated circRNAs in the treatment of glioma patients. Methods: We systematically reviewed the online databases of PubMed, Web of Science, EMBASE, and Cochrane Library to identify studies that explored the relationship between circRNA expression and clinicopathological and prognostic factors in glioma through April 11, 2020. The quality of the included studies was evaluated by the Newcastle-Ottawa Scale (NOS) checklists. Clinicopathological features were assessed by pooled odds ratios (ORs) and 95% confidence intervals (CIs), and overall survival (OS) was assessed by hazard ratios (HRs) and 95% CIs. Results: Twenty-four eligible studies, including 22 studies of clinicopathological features, 1 diagnostic study, and 18 studies of prognosis, that included a total of 1390 patients were ultimately included in this study. Meta-analysis showed that highly expressed oncogenic circRNAs were significantly related to poor clinicopathological features (age: P =0.026; tumor size: P≤0.001; tumor grade: P≤0.001; KPS: P =0.012) and worse overall survival (OS) (HR = 2.01, 95% CI: 1.61-2.50, P≤0.001). Moreover, we found that highly expressed tumor-suppressor circRNAs were related to better clinicopathological features (gender: P =0.042; age: P =0.014; tumor size: P =0.022; tumor grade: P≤0.001) and longer OS (HR = 2.70, 95% CI: 1.82-3.99, P≤0.001). Conclusions: In conclusion, there is a significant correlation between the dysregulated expression of circRNAs and the clinicopathology and prognosis of glioma patients.

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“…Circular RNA (circRNA) is another kind of ncRNA comprising covalently closed single-stranded loops. Recent advances in RNA sequencing and bioinformatics tools have led to the discovery and identification of thousands of circRNAs and validated their important roles in the pathogenesis of cancers [ 18 , 19 , 20 , 21 ]. They can mainly function as ceRNAs to modulate gene transcription by interacting with miRNAs or lncRNAs, sponging miRNAs, or RNA-binding proteins, and rarely can be translated into proteins [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

The Roles of ceRNAs-Mediated Autophagy in Cancer Chemoresistance and Metastasis

Zhang

Lü

2020

Cancers

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Chemoresistance and metastasis are the main causes of treatment failure and unfavorable outcome in cancers. There is a pressing need to reveal their mechanisms and to discover novel therapy targets. Autophagy is composed of a cascade of steps controlled by different autophagy-related genes (ATGs). Accumulating evidence suggests that dysregulated autophagy contributes to chemoresistance and metastasis via competing endogenous RNA (ceRNA) networks including lncRNAs and circRNAs. ceRNAs sequester the targeted miRNA expression to indirectly upregulate ATGs expression, and thereof participate in autophagy-mediated chemoresistance and metastasis. Here, we attempt to summarize the roles of ceRNAs in cancer chemoresistance and metastasis through autophagy regulation.

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Translation and functional roles of circular RNAs in human cancer

Cited by 491 publications

References 108 publications

<p>Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network</p>

<p>Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network</p>

CircRNAs as Potential Biomarkers for the Clinicopathology and Prognosis of Glioma Patients:A Meta-analysis

The Roles of ceRNAs-Mediated Autophagy in Cancer Chemoresistance and Metastasis

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