&lt;p&gt;Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network&lt;/p&gt;

Li, Cui; Long, Hai; Niu, Qin; Jia, Guanqing

doi:10.2147/cmar.s258340

Cited by 26 publications

(30 citation statements)

References 27 publications

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“… 11 Similarly, miR-1182 is uncovered to suppress the multiplication, migration and invasion of NSCLC cells H522 and H1975. 21 Accumulating evidence demonstrates that circRNA can act as ceRNA by targeting miRNA, thereby reducing the inhibitory effect of miRNA on its target. 22 For instance, circWHSC1 modulates MUC1 and hTERT expression through sponging miR-1182, enhancing the multiplication, migration and invasion of ovarian cancer cells, and repressing the apoptosis of ovarian cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“… 24 Reportedly, NOVA2 is remarkably up-modulated in NSCLC tissues, and NOVA2 overexpression markedly facilitates the multiplication, migration and invasion of A549 and SPCA-1 cells. 13 , 21 Mechanistically, the NOVA1/NOVA2 heterodimer enhances the stability of β-catenin mRNA, and therefore augments β-catenin accumulating, which activates the WNT/β-catenin pathway and subsequently enhances the growth, metastasis and EMT of cancer cells, 24 , 25 which suggests that targeting NOVA2 may be one of the potential means to suppress NSCLC progression. In this work, miR-1182 was proved to target NOVA2 mRNA and down-modulate NOVA2 expression, while circ_0001806 indirectly up-modulated NOVA2 by down-modulating miR-1182.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

Yi¹,

Li²,

Wang³

et al. 2021

CMAR

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Background Circular RNAs (circRNAs) are non-coding RNAs with a covalently closed loop. circRNAs affect the progression of diverse cancers. Nonetheless, circ_0001806 expression and function in non-small cell lung cancer (NSCLC) are undefined. Methods qRT-PCR was executed to examine circ_0001806, miR-1182 and NOVA2 mRNA expression levels in NSCLC tissues and cells. CCK-8, EdU, cell scratch test and Transwell assay were conducted to examine the viability, multiplication, migration and invasion of NSCLC cell lines H1650 and HCC827. The binding sites between circ_0001806 and miR-1182, miR-1182 and NOVA2 mRNA were predicted by the circular RNA Interactome and TargetScan databases, and the dual-luciferase reporter gene experiment was employed for verification. Western blot was implemented to examine NOVA2 expression. Results Circ_0001806 expression in NSCLC tissues and cell lines was substantially augmented, while miR-1182 expression was markedly decreased. Circ_0001806 facilitated the multiplication, migration and invasion of H1650 and HCC827 cells, while miR-1182 exerted the opposite effect. Circ_0001806 indirectly enhanced NOVA2 expression by specifically down-modulating miR-1182. Conclusion Circ_0001806 augments NOVA2 expression by targeting miR-1182 to enhance the multiplication, migration and invasion of NSCLC cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

Yi¹,

Li²,

Wang³

et al. 2021

CMAR

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“…Hypoxia enhances circ_0000376 expression and promotes the glycolysis, viability, invasion and migration of NSCLC cells. Manipulated inhibition of circ_0000376 suppresses the progressive activities of hypoxia-induced NSCLC cells both in vitro and in vivo ( Li C. et al, 2020 ).…”

Section: Circrnas and Lung Cancermentioning

confidence: 99%

Insights Into circRNAs: Functional Roles in Lung Cancer Management and the Potential Mechanisms

Feng

Zhou

Wang

et al. 2021

Front. Cell Dev. Biol.

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Lung cancer is the most prevalent cancer globally. It is also the leading cause of cancer-related death because of the late diagnosis and the frequent resistance to therapeutics. Therefore, it is impending to identify novel biomarkers and effective therapeutic targets to improve the clinical outcomes. Identified as a new class of RNAs, circular RNAs (circRNAs) derive from pre-mRNA back splicing with considerable stability and conservation. Accumulating research reveal that circRNAs can function as microRNA (miRNA) sponges, regulators of gene transcription and alternative splicing, as well as interact with RNA-binding proteins (RBPs), or even be translated into proteins directly. Currently, a large body of circRNAs have been demonstrated differentially expressed in physiological and pathological processes including cancer. In lung cancer, circRNAs play multiple roles in carcinogenesis, development, and response to different therapies, indicating their potential as diagnostic and prognostic biomarkers as well as novel therapeutics. In this review, we summarize the multi-faceted functions of circRNAs in lung cancer and the underlying mechanisms, together with the possible future of these discoveries in clinical application.

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“… 13 In addition, circ_0000376 was found to facilitate NSCLC cell glycolysis, viability and metastasis through regulating the miR-1182/NOVA2 network. 14 …”

Section: Introductionmentioning

confidence: 99%

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

Yue¹,

Xu²,

Duan³

et al. 2021

OTT

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Background Circular RNA (circRNA) is a key regulator of cancer, and it has been proved to be involved in the regulation of cancer progression including non-small cell lung cancer (NSCLC). Circ-PITX1 was found to be a significantly upregulated circRNA in NSCLC, and its role and potential mechanism in NSCLC progression deserve further investigation. Methods The expression levels of circ-PITX1, microRNA (miR)-1248 and cyclin D2 (CCND2) were examined by quantitative real-time PCR (qRT-PCR). Cell proliferation, apoptosis, cell cycle process, migration and invasion were determined using cell counting kit 8 (CCK8) assay, colony formation assay, flow cytometry, wound healing assay and transwell assay. Xenograft models were built to explore the role of circ-PITX1 in NSCLC tumor growth in vivo. The glycolysis and glutamine metabolism of cells were assessed by detecting the consumptions of glucose and glutamine, cell extracellular acidification rate (ECAR), and the productions of lactate, α-ketoglutaric acid (α-KG) and ATP. The protein levels of hexokinase 2 (HK-2), glutaminase 1 (GLS1) and CCND2 were tested by Western blot (WB) analysis. Dual-luciferase reporter assay and RIP assay were employed to verify the interaction between miR-1248 and circ-PITX1 or CCND2. Results Circ-PITX1 was upregulated in NSCLC and its silencing could inhibit the proliferation, migration, invasion, cell cycle process, glycolysis, glutamine metabolism, and promote the apoptosis of NSCLC cells in vitro, as well as reduced tumor growth in vivo. In the terms of mechanism, we found that circ-PITX1 could act as a sponge of miR-1248, and miR-1248 could target CCND2. In addition, miR-1248 inhibitor reversed the inhibitory effect of circ-PITX1 knockdown on NSCLC progression. Similarly, CCND2 overexpression also reversed the suppressive effect of miR-1248 on NSCLC progression. Moreover, circ-PITX1 positively regulated CCND2 expression by sponging miR-1248. Conclusion Circ-PITX1 served as a sponge of miR-1248 to promote NSCLC progression by upregulating CCND2.

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<p>Circ_0000376, a Novel circRNA, Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1182/NOVA2 Network</p>

Cited by 26 publications

References 27 publications

Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

Circ_0001806 Promotes the Proliferation, Migration and Invasion of NSCLC Cells Through miR-1182/NOVA2 Axis

Insights Into circRNAs: Functional Roles in Lung Cancer Management and the Potential Mechanisms

Circ-PITX1 Promotes the Progression of Non-Small Cell Lung Cancer Through Regulating the miR-1248/CCND2 Axis

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