Transistor-based immunosensing in human serum samples without on-site calibration

Gutiérrez‐Sanz, Óscar; Haustein, Natalie; Schroeter, Michele; Oelschlaegel, Tobias; Filipiak, Marcin S.; Tarasov, Alexey

doi:10.1016/j.snb.2019.05.043

Cited by 10 publications

(8 citation statements)

References 34 publications

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“…A substantial amount of work has been devoted to pushing the detection limits and sensitivities of biosensors, but little attention has been paid to sensor calibration and validation in real samples. , Only a few calibration-free biosensing approaches ,, have been developed to increase the adaptation and clinical utility of ECBs. For example, Tarasov and co-workers realized an on-site calibration-free biosensor by using “differential” readout of pairs of differently functionalized, antibody-modified electrolyte-gated field-effect transistors . However, the signal transduction mechanism of this sensor depends on the specific chemical reactivity of their target; thus, this strategy is not generalizable to the measurement of other molecules.…”

mentioning

confidence: 99%

“…For example, Tarasov and co-workers realized an on-site calibration-free biosensor by using "differential" readout of pairs of differently functionalized, antibody-modified electrolyte-gated field-effect transistors. 14 However, the signal transduction mechanism of this sensor depends on the specific chemical reactivity of their target; thus, this strategy is not generalizable to the measurement of other molecules.…”

mentioning

confidence: 99%

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Temperature-Alternated Electrochemical Aptamer-Based Biosensor for Calibration-Free and Sensitive Molecular Measurements in an Unprocessed Actual Sample

Chen

Wang

et al. 2021

Anal. Chem.

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Frequently calibrating electrochemical biosensors (ECBs) to obtain acceptable accuracy can be cumbersome for the users. Thus, the achievement of calibration-free operation would effectively lead to commercial applications for ECBs in the real world. Herein, we fabricated a temperature-alternated electrochemical aptamer-based (TAEAB) sensor, producing a cycle of "enhanced-responsive and ∼nonresponsive" state at rapidly alternated interface temperatures (5 and 30 °C, respectively). The ratio of peak currents collected at two temperatures overcomes sensor-to-sensor fabrication variations, obviating sensor calibration prior to use due to its good reproducibility. We then demonstrated the capability of TAEAB sensors for improved, sensitive, and calibration-free measurement of different targets within 7 min, which respectively achieved a detection limit of 0.5 μM procaine in undiluted urine and 1.0 μM adenosine triphosphate in undiluted serum. This generalizable approach ameliorates sensitivity without the complicated amplification step, thus simplifying the operation procedure and reducing the detection time, which will effectively improve the clinical utility of biosensors.

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confidence: 99%

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confidence: 99%

Temperature-Alternated Electrochemical Aptamer-Based Biosensor for Calibration-Free and Sensitive Molecular Measurements in an Unprocessed Actual Sample

Chen

Wang

et al. 2021

Anal. Chem.

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“…extended this approach to detect thyroid-stimulating hormone with antibodies in undiluted serum, 17,22 reporting a three-fold improvement in sensitivity after adding large PEG molecules to the electrode surface. Song et al also employed a PEG coating to detect glial fibrillary acidic protein 23 .…”

Section: The Debye Volume: Limiting Screening Through Surface Enginee...mentioning

confidence: 99%

“…17 Without the PEG coating, the nonspecific devices could not detect PSA and the sensitivity of the aptamer-immobilized devices was reduced 5fold. Gutieŕrez-Sanz et al extended this approach to detect thyroid-stimulating hormone with antibodies in undiluted serum, 18,23 reporting a 3-fold improvement in sensitivity after adding large PEG molecules to the electrode surface. Song et al also employed a PEG coating to detect glial fibrillary acidic protein.…”

Section: The Debye Volume: Limiting Screening Through Surface Enginee...mentioning

confidence: 99%

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Going beyond the Debye Length: Overcoming Charge Screening Limitations in Next-Generation Bioelectronic Sensors

2020

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Electronic biosensors are a natural fit for field-deployable diagnostic devices because they can be miniaturized, mass produced, and integrated with circuitry. Unfortunately, progress in the development of such platforms has been hindered by the fact that mobile ions present in biological samples screen charges from the target molecule, greatly reducing sensor sensitivity. Under physiological conditions, the thickness of the resulting electric double layer is less than 1 nm, and it has generally been assumed that electronic detection beyond this distance is virtually impossible. However, a few recently described sensor design strategies seem to defy this conventional wisdom, exploiting the physics of electrical double layers in ways that traditional models do not capture. In the first strategy, charge screening is decreased by constraining the space in which double layers can form. The second strategy uses external stimuli to prevent double layers from reaching equilibrium, thereby effectively reducing charge screening. In this Perspective, we describe these relatively new concepts and offer theoretical insights into mechanisms that may enable electronic biosensing beyond the Debye length. If these concepts can be further developed and translated into practical electronic biosensors, we foresee exciting opportunities for the next generation of diagnostic technologies.

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Electrochemical Affinity Sensors Using Field Effect Transducer Devices for Chemical Analysis

2021

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The review presents advances and main challenges of the affinity sensors based on field-effect transistors published during the last five years. The different nanomaterial-based field-effect transistors are classified according to the nature of the nanomaterials, beginning by silicon, the "gold-standard" semiconductor, the gallium nitride semiconductor, the organic semiconductors, the silicon nanowires, the inorganic nanomaterials, the carbon nanotubes and the graphene. Due to its exceptional electrical properties, the main works are devoted to graphene. The obtained analytical performances for the detection of biomarkers, of DNA sequences and of miRNA are listed. The relation between the operational conditions -nature of the nanomaterials, procedure of preparation, choice of the receptor molecule, method of immobilization -and the analytical performance are discussed. The perspective of industrialization of these affinity sensors based on field-effect transistors is discussed.

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Transistor-based immunosensing in human serum samples without on-site calibration

Cited by 10 publications

References 34 publications

Temperature-Alternated Electrochemical Aptamer-Based Biosensor for Calibration-Free and Sensitive Molecular Measurements in an Unprocessed Actual Sample

Temperature-Alternated Electrochemical Aptamer-Based Biosensor for Calibration-Free and Sensitive Molecular Measurements in an Unprocessed Actual Sample

Going beyond the Debye Length: Overcoming Charge Screening Limitations in Next-Generation Bioelectronic Sensors

Electrochemical Affinity Sensors Using Field Effect Transducer Devices for Chemical Analysis

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