Transient β-gus and gfp Gene Expression and Viability Analysis of Microprojectile Bombarded Microspores of Brassica napus L.

Nehlin, Lilian; Möllers, Christian; Bergman, Per; Glimelius, Kristina

doi:10.1016/s0176-1617(00)80303-x

Cited by 39 publications

(25 citation statements)

References 32 publications

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“…Microspore-derived embryos of B. napus have been used to examine the biochemical pathways and screen metabolic products (Wiberg et al 1991). Microspores have also proven to be viable transformation targets for the production of transgenic B. napus plants (Nehlin et al 2000).…”

Section: Anther/microspore Culture and Double Haploidsmentioning

confidence: 99%

Advances in in vitro culture of the Brassicaceae crop plants

Park¹,

Ahmed

Kim

et al. 2012

Journal of Plant Biotechnology

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Section: Anther/microspore Culture and Double Haploidsmentioning

confidence: 99%

Advances in in vitro culture of the Brassicaceae crop plants

Park¹,

Ahmed

Kim

et al. 2012

Journal of Plant Biotechnology

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“…A highly efficient doubled haploidy protocol is required for any transformation protocols, making Brassica species ideal candidates. Different microspore transformation methods have been attempted in Brassica including Agrobacterium tumefaciens (Pechan 1989;Dormann et al 2001), particle bombardment (Fukuoka et al 1998;Nehlin et al 2000), microinjection (Jones-Villeneuve et al 1995), and electroporation (Guerche et al 1987;Jardinaud et al 1993). A recent paper combined particle bombardment and Agrobacterium-mediated transformation in B. napus microspores (Abdollahi et al 2009a).…”

Section: Utilization Of Doubled Haploids In Transformationmentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

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102

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The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.

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“…In addition, microspores, MDEs and DH plants are being used for transformation studies. Different approaches have been reported, using Agrobacterium tumefaciens (Dormann et al 2001;Cegielska-Taras et al 2008), particle gun bombardment (Fukuoka et al 1998;Chen and Beversdorf 1994;Nehlin et al 2000), or microinjection (Guerche et al 1987;Jardinaud et al 1993;Huang 1992).…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

et al. 2016

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In Brassica species microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC 1 from a cross between 'Express 617' and 'RS239'. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent 'Express 617' showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of 'Express 617'. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.

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Transient β-gus and gfp Gene Expression and Viability Analysis of Microprojectile Bombarded Microspores of Brassica napus L.

Cited by 39 publications

References 32 publications

Advances in in vitro culture of the Brassicaceae crop plants

Advances in in vitro culture of the Brassicaceae crop plants

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

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