Transient Formation of DNA Strand Breaks During DMSO-Induced Differentiation of Promyelocytic Cell Line, HL-60

Farzaneh, Farzin; Lebby, Robert A.; Brill, David; Shall, Sydney; David, Jean‐Claude; Féon, Sylvie

doi:10.1007/978-3-642-70589-2_60

Cited by 4 publications

(2 citation statements)

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“…The linear sucrose gradients (15-30% for myoblasts and 5-20070 for lymphocytes) also contained 2 M NaCI, 10 mM EDTA and 10 mM Tris-HCI (pH 8.0), but no detergent. Where indicated 30 pg/ml ethidium bromide was included in both the lysis solution and the sucrose gradient to avoid possible effects on the nucleoid sedimentation rates of changes in chromatin composition [24,25]. The gradients were kept in the dark for 15 min at 20°C and then centrifuged at either 10000 rpm in a Beckman SW50.1 rotor for 45 min (muscle cells) or 25000 rpm in a Beckman SW41 rotor for 30 min (lymphocytes).…”

Section: Lymphocyte Culturesmentioning

confidence: 99%

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The dynamic nature of DNA‐strand breaks present in differentiating muscle cells and quiescent lymphocytes

1985

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Cellular d~~e~~a~onin a number of eukaryotic systems is associated with changes in the number of DNAstrand breaks and involves the activity of adenosine d~phosphor~bosyi transferase (ADPRT). DNA-strand breaks are essential For activation of nuclear ADPRT, the activity of which is required for efficient religation OF DNA-strand breaks. In this study we demonstrate the dynamic nature of DNA-strand breaks Formed in the genome of differentiating atian skeletal muscle cells and quiescent human lymphocytes. Inhibition of ADPRT activity blocks DNA-strand ligation in both cell types and leads to the a~umuiation of a higher number OF strand breaks, DNA-strand break ADP-ribosylationSingle-strand DNA breaks are formed at an early stage during the spontaneous differentiation of primary avian skeletal myoblasts in culture [1,2]. Induction of differentiation in murine erythroleukaemic cells [3,4], human promyelocytic leukaemia cells [S] and normal human granulocyte-macrophage progenitor cells [6,7] also results in the early formation of DNA-strand breaks. Quiescent human and murine l~ph~~es contain DNAstrand breaks which are iigated soon after mitogen stimulation (2-20 h), before most indications of activation are manifest f&-10]. In all of these examples alteration in the number af DNA-strand

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Section: Lymphocyte Culturesmentioning

confidence: 99%

“…The position of the nucleoids was then identified by collecting the gradients from the bottom through the flow cell of a recording spectrophotometer set at 260 nm or by direct identification of the nucleoid band against a UV light source in those instances in which the gradient contained ethidium bromide [25].…”

Section: Lymphocyte Culturesmentioning

confidence: 99%