Inhibition of ADP-Ribosyl Transferase Activity Blocks the Stable Integeration of Transfected DNA into the Mammalian Cell Genome

Farzaneh, Farzin; Panayotou, George; Bowler, Lucas D.; Broom, Timothy; Shall, Sydney

doi:10.1007/978-1-4615-8507-7_35

Cited by 1 publication

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“…The nuclear ADPRT, which is totally dependent on the presence of DNA strand-breaks for its activity (12), is involved in a variety of cellular processes which require the ligation of DNA strand-breaks, possibly because it regulates DNA ligase activity (13,14). These processes include DNA excision repair (15), a number of examples of eukaryotic cellular differentiation (2,5,(15)(16)(17)(18)(19), mitogenic activation of quiescent lymphocytes (6-9), sister chrcmatid exchange (20,21), antigenic variation in Trypanoscma brucei (22), and the stable expression of transfected DNA in mammalian host cells (24). For a recent review of ADPRT involvement in cellular processes see references 25 to 28.…”

Section: Several Studies Havementioning

confidence: 99%

Transient formation of DNA strand breaks during the induced differentiation of a human promyelocytic leukaemic cell line, HL-60

1987

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During the induced differentiation of the human promyelocytic leukaemic cell line, HL-60, along the myelocytic lineage, DNA strand-breaks are formed. These breaks which are formed in the face of a proficient DNA repair mechanism, are only transiently maintained and subsequently become religated. The ligation of these breaks requires the activity of the nuclear adenosine diphosphoribosyl transferase (ADPRT). Inhibition of nuclear ADPRT, an enzyme totally dependent on the presence of DNA strand-breaks for its activity and required for efficient DNA repair in eukaryotic cells, blocks the religation of these breaks but not their formation. The inhibition of DNA strand ligation in the differentiating HL-60 cells results in loss of viability and cell death.

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