1987
DOI: 10.1128/mcb.7.10.3830
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Transient expression of the beta interferon promoter in human cells.

Abstract: A human transient expression assay was used to examine the inducible transcriptional activation of beta interferon (IFN-beta) and IFN-alpha 1 promoters in a homologous cellular environment. Use of 293 cells, an adenovirus DNA-transformed human embryonic kidney cell line, permitted Sendai virus-inducible expression of IFN-beta-CAT hybrid gene. Introduction of the simian virus 40 (SV40) enhancer 5' or 3' to the IFN-CAT gene increased basal (uninduced) levels of chloramphenicol acetyltransferase (CAT) activity; i… Show more

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Cited by 19 publications
(31 citation statements)
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“…NF-KB is a multisubunit transcription factor comprising p50, p65, and rel protooncogene products (7) that has been implicated in the regulation of the IFN-3 gene promoter by dsRNA (8)(9)(10). In many unstimulated cells, NF-KB is found localized to the cytoplasm as a latent heterodimeric complex bound to its subunit-specific inhibitor IKB (also called MAI)-3) (11).…”
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confidence: 99%
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“…NF-KB is a multisubunit transcription factor comprising p50, p65, and rel protooncogene products (7) that has been implicated in the regulation of the IFN-3 gene promoter by dsRNA (8)(9)(10). In many unstimulated cells, NF-KB is found localized to the cytoplasm as a latent heterodimeric complex bound to its subunit-specific inhibitor IKB (also called MAI)-3) (11).…”
mentioning
confidence: 99%
“…Human full-length PKR was expressed as a glutathione S-transferase (GST) fusion in the expression vector pGEX-2T and purified from bacteria by using glutathione-Sepharose chromatography (18). 1KB-a, p50, and p65 (10)(11)(12) were produced in a similarfashion except that the GST portion ofthese fusion proteins was cleaved with thrombin and removed by centrifugation (the molecular mass of the bacterially produced IKB-a protein was 37 kDa).…”
mentioning
confidence: 99%
“…We previously described a human transient expression system that accurately reflected the virus-induced activation of the IFN-P promoter (49). Expression of IFN-,-Bchloramphenicol acetyltransferase (CAT) hybrid genes was dependent on induction by virus; however, no expression from the IFN-a-CAT hybrid gene was detectable, indicating that human epithelioid cells lack a factor required for the expression of the IFN-a promoter.…”
mentioning
confidence: 99%
“…TAg also increases production of mRNA from other viral and cellular promoters (1,6,40). We asked whether TAg could likewise trans activate by similar indirect mechanisms the IFN promoter, given that IFN regulation is tightly controlled by positive and negative regulatory interactions (9,13,27,49,51). The activities of the IFN-a1 and IFN-, promoters were first compared in the presence and absence of viral TAg (Fig.…”
mentioning
confidence: 99%
“…The positive regulatory domain I (PRDI) is located between nucleotides -77 and -64 from the start site of transcription, while another element (PRDII) is located between -66 and -55 (7)(8)(9)(10). A third element (PRDIII) is located between -90 and -78 (11)(12)(13)(14)(15).…”
mentioning
confidence: 99%