Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

Hesketh, Emma L.; Knight, John R. P.; Wilson, Rosemary H C; Chong, J.; Coverley, Dawn

doi:10.4161/15384101.2014.980647

Cited by 9 publications

(9 citation statements)

References 52 publications

(48 reference statements)

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“…5), thus indicating that all these cells were engaged in the cell cycle. Indeed, the MCM complex is loaded onto chromatin at replication origins during late mitosis or G1 phase to form pre-replicative complexes (Blow and Dutta, 2005;Hesketh et al, 2015), and it is absent in quiescent cells (Namdar and Kearsey, 2006). These data were corroborated by the intense incorporation of BrdU into the duct inner cells and in cells of the duct ciliated epithelium.…”

Section: Discussionmentioning

confidence: 81%

“…The minichromosome maintenance MCM6 protein is loaded onto chromatin at replication origins during late mitosis or G1 phase to form pre-replicative complexes (Blow and Dutta, 2005;Hesketh et al, 2015). MCM6 is conserved through the eukaryotes as illustrated by the homology between mollusk and toad (X. laevis) sequences (Fig.…”

Section: Dna Replication In the Gonad At An Early Stage Of The Oystermentioning

confidence: 99%

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Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers

Cavelier

Cau

Morin

et al. 2017

Journal of Experimental Biology

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While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster sexual cycle. Moreover, Vasa, a cytoplasmic marker for germline cells, was also detected in the gonad acini and duct cells, thus confirming that germline cells were abundant early on. In addition, the binding of the minichromosome maintenance MCM6 protein to chromatin indicated the gonad acini and duct cells were engaged in the cell cycle. DNA replication was indeed confirmed by an abundant incorporation of BrdU into the duct cell chromatin. Finally, proliferation of acini and duct cells was demonstrated by the chromatin-bound Ser10-phosphorylated histone H3, a mitotic marker. The markers for the cell cycle and mitosis used here thus indicate that acini and duct cells were already actively dividing early during the oyster sexual cycle. In addition, together with the stem cell markers, these data reveal that the epithelium delimiting the duct outer edge contains a dynamic population of undifferentiated cells.

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Section: Discussionmentioning

confidence: 81%

Section: Dna Replication In the Gonad At An Early Stage Of The Oystermentioning

confidence: 99%

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers

Cavelier

Cau

Morin

et al. 2017

Journal of Experimental Biology

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“…Remarkably, this nuclease-insoluble fraction is characterized by the presence of Top2, Rad53, Sgs1, the ORC, and the Cdc7-regulator Dbf4 (Frei and Gasser, 2000;Pasero et al, 1999), which are involved in replication fork stability under replication stress (Branzei and Foiani, 2010). In mammalian cells, replication origins and components of the pre-RC, including MCM, are associated during G1 and early S phases with the nuclear scaffold, defined as a salt-resistant or nuclease-insoluble nuclear fraction (Hesketh et al, 2015;Wilson and Coverley, 2013). According to those data, the DNA would be spooled through static replication machinery, and the newly synthesized DNA would be extruded as two loops (Wilson and Coverley, 2013).…”

Section: Discussionmentioning

confidence: 99%

Physical interactions between MCM and Rad51 facilitate replication fork lesion bypass and ssDNA gap filling by non-recombinogenic functions

Cabello-Lobato¹,

González-Garrido²,

Cano-Linares³

et al. 2021

Cell Reports

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“…In addition, E2F and PCNA integrate the cell cycle with DNA replication [49,50]. In eukaryotic cells, the MCM complex (known as DNA replication licensing) controls the once-per-cell cycle DNA replication process [51,52]. Therefore, targeting DNA replication stress is one strategy for cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

The Role of p53-Mediated Signaling in the Therapeutic Response of Colorectal Cancer to 9F, a Spermine-Modified Naphthalene Diimide Derivative

Gao

Wang

et al. 2020

Cancers

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Colorectal cancer (CRC) is one of the most prevalent cancers due to its frequency and high rate of mortality. Polyamine-vectorized anticancer drugs possess multiple biological properties. Of these drugs, 9F has been shown to inhibit tumor growth and the metastasis of hepatocellular carcinoma. This current study aims to investigate the effects of 9F on CRC and determine its molecular mechanisms of action. Our findings demonstrate that 9F inhibits CRC cell growth by inducing apoptosis and cell cycle arrest, and suppresses migration, invasion and angiogenesis in vitro, resulting in the inhibition of tumor growth and metastasis in vivo. Based on RNA-seq data, further bioinformatic analyses suggest that 9F exerts its anticancer activities through p53 signaling, which is responsible for the altered expression of key regulators of the cell cycle, apoptosis, the epithelial-to-mesenchymal transition (EMT), and angiogenesis. In addition, 9F is more effective than amonafide against CRC. These results show that 9F can be considered as a potential strategy for CRC treatment.

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Transient association of MCM complex proteins with the nuclear matrix during initiation of mammalian DNA replication

Cited by 9 publications

References 52 publications

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers

Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers

Physical interactions between MCM and Rad51 facilitate replication fork lesion bypass and ssDNA gap filling by non-recombinogenic functions

The Role of p53-Mediated Signaling in the Therapeutic Response of Colorectal Cancer to 9F, a Spermine-Modified Naphthalene Diimide Derivative

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