Transglycosylation specificity of glycosyl donors in transglycosylation of stevioside catalysed by cyclodextrin glucanotransferase

Lu, Tong; Xia, Yongmei

doi:10.1016/j.foodchem.2014.03.017

Cited by 20 publications

(24 citation statements)

References 19 publications

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“…The transglucosylation pathway of stevioside with hydrolyzed/gelatinized starch involves cyclodextrin transglucosylation and hydrolysis pathway of starches [17]. Therefore, the influence of microwave irradiation on the transglucosylation pathway of cyclodextrin was noted in Figure 4.…”

Section: The Transglycosylation Pathway Affected By the Microwave Irrmentioning

confidence: 97%

“…Detailed calculation or analysis strategy in this experiment was adapted from the previous work [17]. In thermo generated transglucosylation of stevioside with cyclodextrins, there was a transformation between α-CD to β-CD [17]; and it was the intermediate of reducing sugar instead of glucose produced from cyclodextrins donated the glucosyl to stevioside.…”

Section: The Transglycosylation Pathway Affected By the Microwave Irrmentioning

confidence: 99%

“…Therefore, in decades, many efforts have been made to enzymatic modification of stevioside to improve its edulcorant quality through transglycosylation or hydrolysis of stevioside [10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…However, enzymatic transglycosylation of stevioside usually takes longer reaction time and concerns thermo-deactivation of the enzyme, yet its reaction pathway has been rarely studied [17].…”

Section: Introductionmentioning

confidence: 99%

“…Later, reaction pathways of transglycosylation of stevioside with starch catalyzed by CGTs were proposed that α/β-cyclodextrin (CD) performed transglycosylation via coupling to produce intermediates of reducing sugar and followed by disproportionation with stevioside; while starches may perform the transglycosylation combined the CDs' pathway and hydrolysis pathway of starches [17]. However, except the speeding, no evidences of how microwave affect the reaction selectivity, or the pathways have not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

Zhang

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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Section: The Transglycosylation Pathway Affected By the Microwave Irrmentioning

confidence: 97%

Section: The Transglycosylation Pathway Affected By the Microwave Irrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

Zhang

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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Heterologous expression of β-γ-type cyclodextrin glycosyltransferase of newly isolated alkaliphilicBacillussp. SD5 inPichia pastoris

Kabacaoğlu

Budak

2017

Starch - Stärke

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Cyclodextrin glycosyltransferase (CGTase) enzyme is used industrially to obtain cyclodextrins. In this study, a newly isolated alkaliphilic CGTase producing Bacillus sp. SD5 was used as the source organism for the gene encoding for CGTase. The gene was amplified and sequenced in preparation for cloning into Pichia pastoris for methanol‐induced expression and extracellular secretion. Three strategies were employed in designing the amplification primers for obtaining the fragments to be used in transformation. Hence, three P. pastoris X33 clones were obtained: N2 (with natural gene sequence), S3 (also encoding the c‐myc epitope and polyhistidine tail), and M5 (encoding the polyhistidine tail). Extracellular CGTase activity was monitored throughout fermentation for each clone. The molecular weights of the enzymes were in the range of 85–90 kDa and all three clones showed maximum CGTase activity upon 24 h of induced fermentation. The CGTase enzyme obtained from the M5 clone had Km and Vmax values 13.59 mg/mL and 1153 µmol/(mg min), respectively. The enzyme was purified and displayed optimum activity at 50°C and pH 6 and pH 9. HPLC analysis of reaction products following 10 min of incubation in a reaction solution containing 4% starch showed 852.5 mg/L β‐cyclodextrin and 89.6 mg/L γ‐cyclodextrin being produced.

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Recent progress on biological production of α-arbutin

Zhu

Tian

Zhang

et al. 2018

Appl Microbiol Biotechnol

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Arbutin, a glucoside of hydroquinone, is used as a powerful skin lightening agent in the cosmeceutical industry because of its strong inhibitory effect on the human tyrosinase activity. It is a natural compound occurring in a number of plants, with a β-anomeric form of the glycoside bond between glucose and hydroquinone. α-Arbutin, which glycoside bond is generated with α-anomeric form, is the isomer of natural arbutin. α-Arbutin is generally produced by transglucosylation of hydroquinone by microbial glycosyltransferases. It is interesting that α-arbutin is found to be over 10 times more effective than arbutin, and thus biological production of α-arbutin attracts increasing attention. Seven different microbial enzymes have been identified to be able to produce α-arbutin, including α-amylase, sucrose phosphorlase, cyclodextrin glycosyltransferase, α-glucosidase, dextransucrase, amylosucrase, and sucrose isomerase. In this work, enzymatic and microbial production of α-arbutin is reviewed in detail.

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Transglycosylation specificity of glycosyl donors in transglycosylation of stevioside catalysed by cyclodextrin glucanotransferase

Cited by 20 publications

References 19 publications

The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

Heterologous expression of β-γ-type cyclodextrin glycosyltransferase of newly isolated alkaliphilicBacillussp. SD5 inPichia pastoris

Recent progress on biological production of α-arbutin

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