The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

Zhang, Yandong; Li, Wei; Lu, Tong; Xia, Yongmei

doi:10.1016/j.molcatb.2015.07.012

Cited by 15 publications

(15 citation statements)

References 26 publications

(37 reference statements)

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“…Therefore, significant research effort has been focused on the addition of glycosyl moieties in steviol glycosides, aiming at the improvement of their organoleptic properties. Cyclodextrin glucanotransferases (CGTases, 1,4-α-d-glucan: 1,4-α-d-glucopyranosyltransferase, cyclizing, EC 2.4.1.19) are the most common enzymes used for steviol glycosides transglycosylation, introducing α-1,4 glycosidic bonds with very high yields, exceeding 70% ( Li et al, 2013 ; Yu et al, 2015 ; Zhang et al, 2015 ). However, the hydrolysis of α- 1,4 bonds by human amylases can increase the caloric value of the modified steviosides, resulting in a final product with low appeal to target consumer groups, e.g., diabetics ( Gerwig et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Zerva

Chorozian

Kritikou

et al. 2021

Front. Bioeng. Biotechnol.

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Stevia rebaudiana Bertoni is a plant cultivated worldwide due to its use as a sweetener. The sweet taste of stevia is attributed to its numerous steviol glycosides, however, their use is still limited, due to their bitter aftertaste. The transglycosylation of steviol glycosides, aiming at the improvement of their taste, has been reported for many enzymes, however, glycosyl hydrolases are not extensively studied in this respect. In the present study, a β-glucosidase, MtBgl3a, and a β-galactosidase, TtbGal1, have been applied in the transglycosylation of two steviol glycosides, stevioside and rebaudioside A. The maximum conversion yields were 34.6 and 33.1% for stevioside, while 25.6 and 37.6% were obtained for rebaudioside A conversion by MtBgl3a and TtbGal1, respectively. Low-cost industrial byproducts were employed as sugar donors, such as cellulose hydrolyzate and acid whey for TtbGal1- and MtBgl3a- mediated bioconversion, respectively. LC-HRMS analysis identified the formation of mono- and di- glycosylated products from stevioside and rebaudioside A. Overall, the results of the present work indicate that both biocatalysts can be exploited for the design of a cost-effective process for the modification of steviol glycosides.

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Section: Introductionmentioning

confidence: 99%

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Zerva

Chorozian

Kritikou

et al. 2021

Front. Bioeng. Biotechnol.

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“…This finding is comparable with our highest stevioside conversion (83 ± 5%) as well as our highest reb A concentrations. Stevioside conversions of between 60% and 70% are achieved using non‐conventional microwave‐assisted bioconversions [ 24 , 25 ]. Rebaudioside A yields at a pH of 3, temperature of 15°C and sucrose concentration of 5 g/L were 0.331 and 0.665 for dried and lyophilized plant extracts, respectively.…”

Section: Resultsmentioning

confidence: 99%

Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13

Czinkóczky

Németh

2021

Engineering in Life Sciences

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Stevia rebaudiana is a sweet herbaceous perennial plant, which is frequently used in the preparation of plant-based sweeteners. The demand for such sweeteners continues to increase due to purposeful nutrition and modern-day metabolic syndromes. More than 20 types of steviol glycosides provide a sweet taste, which are more than 300 times sweeter than sucrose. They are formed of two main components, namely stevioside and rebaudioside A. Only a handful of studies have dealt with Stevia rebaudiana leaf extracts, the conversion of pure stevioside into the preferred rebaudioside A is more common. The aim of this study was to enrich the rebaudioside A content of Stevia rebaudiana leaf extract using enzymatic bioconversion by applying fermented cyclodextrin glycosyltransferase from Bacillus licheniformis DSM13. Two differently processed plant materials, namely dried and lyophilized Stevia rebaudiana plants, were extracted and compared. Following the bioconversion, the rebaudioside A content was on average doubled. The maximum increase was fivefold with a 70-80% conversion of the stevioside.

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“…Moreover, glycosylated SGs may lead to improved bioavailability and solubility of water-soluble components [18]. Previously published studies have indicated that rubusoside is an effective solubilizer, and a 10% rubusoside aqueous solution (w/v) leads to enhanced solubility of curcumin (0.6-2.3 mg/mL), but its industrial application is not cost-effective [19,20]. Curcuminoids are practically reported to be insoluble in acidic mediums and may be hydrolyzed into feruloylmethane, ferulic acid, and yellowish-brown condensation products.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Microwave-Assisted Green Method for Enhanced Solubilization of Water-Soluble Curcuminoids Prepared Using Steviol Glycosides

Ryu

Lee

et al. 2021

Foods

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In this study, the optimization and modeling of microwave-assisted extraction (MAE) of water-soluble curcuminoids prepared using novel steviol glycosides (SGs) was carried out using four independent process variables at varying levels—X1: microwave power (50–200 W), X2: stevioside concentration (50–200 mg/mL), X3: curcumin concentration (20–200 mg/mL), and X4: time (1–10 min)—in response surface methodology configuration. Moreover, the effects of stevioside, as the most cost-effective natural solubilizer, were also evaluated. The water solubility of curcuminoids increased from 11 to 1320 mg/L with the addition of stevioside as a natural solubilizer. Moreover, microwave heating synergistically with stevioside addition significantly (p < 0.05) increased the solubility up to 5400 mg/L. Based on the results, the optimum conditions providing the maximum solubilization of 16,700 mg/L were 189 W microwave power, 195 g/L stevioside concentration, 183 g/L curcuminoid concentration, and 9 min of incubation time. Moreover, MAE of curcuminoids using SGs might render a significant advantage for its wide-scale application to solubilizing the multitude of insoluble functional flavonoids in fruits, plants, and food materials.

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The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

Cited by 15 publications

References 26 publications

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13

Optimization of Microwave-Assisted Green Method for Enhanced Solubilization of Water-Soluble Curcuminoids Prepared Using Steviol Glycosides

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