Transglycosylation of stevioside to improve the edulcorant quality by lower substitution using cornstarch hydrolyzate and CGTase

The effect of microwave irradiation on transglycosylation pathway of stevioside with starches or cyclodextrins catalyzed by a cyclodextrin glucanotransferase

“…Another interesting result is, the St conversion reached the highest when the mass ratio of cornstarch to St was 0.5:1 (w/w) ( Figure 2D) instead of 1:1 (w/w) that was needed under conventional heating [20]. Moreover, contrasting to that in conventional heating, higher mass ratio leading a decrease to St conversion and yield of glucosylated products under microwave irradiation [20].…”

“…There are two possibilities can cause this: thermal/irradiated deactivation of the enzyme and the equilibrium between the hydrolysis and glucosylation; which will be verified with extra enzyme loading experiment in the next section. In addition, it took 5 min ( Figure 2C) to obtain 36% of stevioside conversion that would cost 60 min under conventional heating [20].…”

“…Therefore, in decades, many efforts have been made to enzymatic modification of stevioside to improve its edulcorant quality through transglycosylation or hydrolysis of stevioside [10][11][12][13][14][15][16][17][18][19][20].…”

“…In our previous work, transglycosylation of stevioside was investigated using a commercial cyclodextrin glucanotransferase (CGTase) Toruzyme 3.0L and hydrolyzed cornstarch; with controlled parameters, the product was mainly composed of mono-and di-glucosylated stevioside while the highest stevioside conversion reached 77.1% [20]. Later, reaction pathways of transglycosylation of stevioside with starch catalyzed by CGTs were proposed that α/β-cyclodextrin (CD) performed transglycosylation via coupling to produce intermediates of reducing sugar and followed by disproportionation with stevioside; while starches may perform the transglycosylation combined the CDs' pathway and hydrolysis pathway of starches [17].…”

Análisis De Las Fuerzas Del Mercado Dendroenergético en La Vereda Cajete Del Municipio De Popayán

“…Rbd-A, presenta una unidad de glucosa extra en la posición C-13 en comparación con el esteviósido; por lo tanto, posee propiedades superiores en términos de dulzor y sabor [28,31].…”