Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature

Kayıhan, Doğa Selin; Kayıhan, Ceyhun; Çiftçi, Yelda Özden

doi:10.1080/15226514.2020.1786795

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…Interestingly, a recent transgenic tobacco plant, Nicotiana tabacum, was reported using the bacterial Ntr gene (nitroreductase) to degrade DNT pollutants at 4 °C [ 115 ]. The objective of the study was to produce a transgenic plant used in the phytoremediation treatment to remediate DNT pollutants during the winter season.…”

Section: Other Potential Applicationsmentioning

confidence: 99%

Bibliometric Analysis of Hydrocarbon Bioremediation in Cold Regions and a Review on Enhanced Soil Bioremediation

Yap

Zakaria

Zulkharnain

et al. 2021

Biology

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The increased usage of petroleum oils in cold regions has led to widespread oil pollutants in soils. The harsh environmental conditions in cold environments allow the persistence of these oil pollutants in soils for more than 20 years, raising adverse threats to the ecosystem. Microbial bioremediation was proposed and employed as a cost-effective tool to remediate petroleum hydrocarbons present in soils without significantly posing harmful side effects. However, the conventional hydrocarbon bioremediation requires a longer time to achieve the clean-up standard due to various environmental factors in cold regions. Recent biotechnological improvements using biostimulation and/or bioaugmentation strategies are reported and implemented to enhance the hydrocarbon removal efficiency under cold conditions. Thus, this review focuses on the enhanced bioremediation for hydrocarbon-polluted soils in cold regions, highlighting in situ and ex situ approaches and few potential enhancements via the exploitation of molecular and microbial technology in response to the cold condition. The bibliometric analysis of the hydrocarbon bioremediation research in cold regions is also presented.

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Section: Other Potential Applicationsmentioning

confidence: 99%

Bibliometric Analysis of Hydrocarbon Bioremediation in Cold Regions and a Review on Enhanced Soil Bioremediation

Yap

Zakaria

Zulkharnain

et al. 2021

Biology

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“…Throughout history, wars and armed conflicts have produced and released billions of tons of trinitrotoluene (TNT) and its hazardous precursor, 2,4‐dinitrotoluene (2,4‐DNT), into the environment, causing significant concern. [ 1 , 2 ] 2,4‐DNT is widely used in the production of explosives, as well as in the manufacture of herbicides, plastics, and automobile airbags, resulting in significant environmental pollution and carbon emissions, posing a severe threat to public health. [ 3 , 4 , 5 ] Many published studies have established the carcinogenic effects of 2,4‐DNT on humans [ 6 ] and mutagenic toxicity to microorganisms, algae, fish, and plants.…”

Section: Introductionmentioning

confidence: 99%

“…[ 3 , 4 , 5 ] Many published studies have established the carcinogenic effects of 2,4‐DNT on humans [ 6 ] and mutagenic toxicity to microorganisms, algae, fish, and plants. [ 2 , 7 ] Recently, the World Health Organization and the United States Environmental Protection Agency classified 2,4‐DNT as a Group 2B human carcinogen and priority pollutant, respectively [ 8 , 9 ] Consequently, the increasing concern about 2,4‐DNT pollution has sparked a frenzy to eliminate it from the environment.…”

Section: Introductionmentioning

confidence: 99%

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Gao,

Li,

Zhu

et al. 2023

Advanced Science

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Pervasive environmental contamination due to the uncontrolled dispersal of 2,4‐dinitrotoluene (2,4‐DNT) represents a substantial global health risk, demanding urgent intervention for the removal of this detrimental compound from affected sites and the promotion of ecological restoration. Conventional methodologies, however, are energy‐intensive, susceptible to secondary pollution, and may inadvertently increase carbon emissions. In this study, a 2,4‐DNT degradation module is designed, assembled, and validated in rice plants. Consequently, the modified rice plants acquire the ability to counteract the phytotoxicity of 2,4‐DNT. The most significant finding of this study is that these modified rice plants can completely degrade 2,4‐DNT into innocuous substances and subsequently introduce them into the tricarboxylic acid cycle. Further, research reveals that the modified rice plants enable the rapid phytoremediation of 2,4‐DNT‐contaminated soil. This innovative, eco‐friendly phytoremediation approach for dinitrotoluene‐contaminated soil and water demonstrates significant potential across diverse regions, substantially contributing to carbon neutrality and sustainable development objectives by repurposing carbon and energy from organic contaminants.

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Exploring Multifaceted Roles of Nitroreductases in Bacterial, Animal and Plant Biosystems and their Detection using Fluorescent Probes

Verma,

Singla,

Bhadwal

et al. 2024

ChemistrySelect

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Nitroreductases are the enzymes that can convert nitroaromatic substances including explosives like TNT to their corresponding nitrite, hydroxylamine or amino derivatives with reduced NADH or NADPH as an electron donor. A wide range of nitroreductases are known to present in bacterial genomes that are explored for their potential in the reduction of nitroaromatic compounds and they are used in converting prodrugs to toxic hydroxylamine derivatives causing tumor cell killing. Likewise, in cancer cells, the low oxygen condition called hypoxia is a characteristic of solid tumors and during hypoxia nitroreductase gene expression occurs. Moreover, due to the ability of nitroreductases to reduce nitroaromatic compounds, phytoremediation potential of plants was inspected by expressing bacterial nitroreductase genes in plants through genetic engineering. Due to its diversity and widespread applications in different biosystems, its detection has gained momentum among the scientific community. Designing fluorescent probes for detecting nitroreductases has emerged as a beneficial tool compared to conventional monitoring approaches due to their high sensitivity, selectivity, and real‐time monitoring ability. This review aims to provide an understanding of the activity and the status of nitroreductases in bacteria, plants and animal cells as well as the significance of fluorescent imaging‐related studies by employing recently designed probes.

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Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature

Cited by 5 publications

References 39 publications

Bibliometric Analysis of Hydrocarbon Bioremediation in Cold Regions and a Review on Enhanced Soil Bioremediation

Bibliometric Analysis of Hydrocarbon Bioremediation in Cold Regions and a Review on Enhanced Soil Bioremediation

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Exploring Multifaceted Roles of Nitroreductases in Bacterial, Animal and Plant Biosystems and their Detection using Fluorescent Probes

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