Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate

Chhapekar, Sushil Satish; Sanagala, Raghavendrarao; Pavan, Gadamchetty; Chopperla, Ramakrishna; Singh, Vivek Kumar; Phanindra, Mullapudi Lakshmi Venkata; Gurusamy, Dhandapani; Sreevathsa, Rohini; Kumar, Polumetla Ananda

doi:10.1007/s00299-014-1732-2

Cited by 38 publications

(33 citation statements)

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“…In previous reports related to glyphosate-tolerant rice, either hpt gene or bar gene was used as the selectable marker gene, but glyphosate-tolerant EPSPS genes were rarely used as the selectable marker gene in rice transformation directly (Tian et al, 2013; Chandrasekhar et al, 2014; Deng et al, 2014; Chhapekar et al, 2015). Although G6 gene has been used as the selectable marker gene, the PCR positive ratio of the transformants was relatively low and the number of transformants with a single copy of foreign gene was rather limited (Zhao et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

et al. 2016

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Glyphosate is the most widely used herbicide for its low cost and high efficiency. However, it is rarely applied directly in rice field due to its toxicity to rice. Therefore, glyphosate-tolerant rice can greatly decrease the cost of rice production and provide a more effective weed management strategy. Although, several approaches to develop transgenic rice with glyphosate tolerance have been reported, the agronomic performances of these plants have not been well evaluated, and the feasibility of commercial production has not been confirmed yet. Here, a novel glyphosate-tolerant gene cloned from the bacterium Isoptericola variabilis was identified, codon optimized (designated as I. variabilis-EPSPS*), and transferred into Zhonghua11, a widely used japonica rice cultivar. After systematic analysis of the transgene integration via PCR, Southern blot and flanking sequence isolation, three transgenic lines with only one intact I. variabilis-EPSPS* expression cassette integrated into intergenic regions were identified. Seed test results showed that the glyphosate tolerance of the transgenic rice was about 240 times that of wild type on plant medium. The glyphosate tolerance of transgenic rice lines was further evaluated based on comprehensive agronomic performances in the field with T3 and T5generations in a 2-year assay, which showed that they were rarely affected by glyphosate even when the dosage was 8400 g ha−1. To our knowledge, this is the first demonstration of the development of glyphosate-tolerant rice lines based on a comprehensive analysis of agronomic performances in the field. Taken together, the results suggest that the selected glyphosate-tolerant rice lines are highly tolerant to glyphosate and have the possibility of commercial release. I. variabilis-EPSPS* also can be a promising candidate gene in other species for developing glyphosate-tolerant crops.

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Section: Discussionmentioning

confidence: 99%

“…sp (Zhao et al, 2011; Tian et al, 2013, 2015; Chandrasekhar et al, 2014; Deng et al, 2014; Chhapekar et al, 2015; Yi et al, 2016). Transgenic rice in these reports presented normal morphology 7 or 10 d after glyphosate treatment, suggesting that these transgenic rice plants are tolerant to glyphosate.…”

Section: Introductionmentioning

confidence: 99%

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

et al. 2016

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“…Gene methylation or occurrence of any other homologous gene in host genome may lead to complete or partial silencing of the transgene as well. One percent glyphosate spray (v/v) used for herbicide assay in this study was equivalent to the concentration used by Chhapekar et al (2015) and remarkably high in comparison to 0.1 to 0.5% (v/v) concentration used by Te et al (2011). HR tobacco plants expressing CP4-EPSPS gene conferred variable resistance against glyphosate at routine field dosage.…”

Section: Discussionmentioning

confidence: 92%

Genetically transformed tobacco plants expressing synthetic EPSPS gene confer tolerance against glyphosate herbicide

Imran

Asad

Barboza

et al. 2017

Physiol Mol Biol Plants

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Glyphosate quashes the synthesis of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme which intercedes the functioning of shikimate pathway for the production of aromatic amino acids. Herbicide resistant crops are developed using glyphosate insensitive EPSPS gene isolated from Agrobacterium sp. strain CP4, which give farmers a sustainable weed control option. Intentions behind this study were to design and characterize the synthetic herbicide resistant CP4-EPSPS gene in a model plant system and check the effectiveness of transformed tobacco against application of glyphosate. Putative transgenic plants were obtained from independent transformation events, and stable plant transformation, transgene expression and integration were demonstrated respectively by PCR, qRT-PCR and Southern hybridization. Gene transcript level and gene copy number (1-4) varied among the tested transgenic tobacco lines. Herbicide assays showed that transgenic plants were resistant to glyphosate after 12 days of spraying with glyphosate, and EPSPS activity remained at sufficient level to withstand the spray at 1000 ppm of the chemical. T 1 plants analyzed through immunoblot strips and PCR showed that the gene was being translated into protein and transmitted to the next generation successfully. This codon optimized synthetic CP4-EPSPS gene is functionally equivalent to the gene for glyphosate resistance available in the commercial crops and hence we recommend this gene for transformation into commercial crops.

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“…Durante el año 2010 se sembraron soya, maíz, canola, algodón, remolacha y alfalfa con esta característica, y el área sembrada alcanzó los 89,3 millones de hectáreas (James, 2010). Desde la entrada en producción de la soya transgénica Roundup Ready en 1996, introducida por la corporación Monsanto, se han obtenido nuevos organismos transgénicos resistentes al glifosato, en un intento por lograr una elevada productividad, bajos costos de producción, reducir la necesidad de labores y asegurar un fácil control de malezas a través del uso de herbicidas (Chhapekar, Raghavendrarao, Pavan, Ramakrishna, Singh, Phanindra, …& Kumar, 2015;Liu, Cao, Chen, Zhang, Ren, Lu, … & Wang, 2015) En 2014, el 82% (90,7 millones de hectáreas) de los 111 millones de hectáreas de soya plantadas en el mundo fueron transgénicas. Se plantaron 25,1 millones de hectáreas de algodón Bt, lo que significa un 68% de los 37 millones de hectáreas globales de algodón.…”

Section: Héctor E | Pérez S | Moreira R | Mille Bunclassified

Perspectivas futuras e impacto social de las biotecnologías vegetales

et al. 2017

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RESUMENLas investigaciones en biotecnología vegetal han conducido a la introducción en la agricultura de numerosos productos científicos, entre los que se destacan el mejoramiento genético y la multiplicación acelerada de especies vegetales, la conservación de germoplasma y las plantas transgénicas. Al mismo tiempo, se ha mantenido un constante debate sobre los beneficios y costos sociales de las aplicaciones biotecnológicas, principalmente dirigido hacia la inserción de los organismos genéticamente modificados en la agricultura. Este artículo tiene como objetivo hacer un balance de las perspectivas actuales y futuras de la aplicación de las biotecnologías vegetales en la agricultura, y de las implicaciones de su empleo para la sociedad. Se discuten los resultados más recientes obtenidos en esta área del conocimiento. Se concluye que la biotecnología vegetal ha demostrado su valor científico y aplicación práctica, aunque subsisten desigualdades en la propiedad sobre los avances y las ganancias obtenidas, las cuales tienden a mantenerse en el futuro.PALABRAS CLAVE: biotecnología vegetal, cultivo de tejidos, conservación de germoplasma, transgénesis. ABSTRACTResearch in plant biotechnology has led to the introduction of numerous scientific agriculture products, among which breeding and rapid multiplication of plant species, conservation of germplasm and transgenic plants are included. At the same time, it has remained a constant debate about the benefits and social costs of biotechnological applications, mainly directed towards the insertion of genetically modified organisms in agriculture. This article aims to take stock of current and future perspectives on application of plant biotechnologies in agriculture and their implications of use to society. The most recent results in this area of knowledge are discussed. It is concluded that plant biotechnology has demonstrated its scientific value and practical application, although there are still inequalities in the ownership of progress and profit obtained, which tend to remain in the future.

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Transgenic rice expressing a codon-modified synthetic CP4-EPSPS confers tolerance to broad-spectrum herbicide, glyphosate

Cited by 38 publications

References 35 publications

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

Development of Novel Glyphosate-Tolerant Japonica Rice Lines: A Step Toward Commercial Release

Genetically transformed tobacco plants expressing synthetic EPSPS gene confer tolerance against glyphosate herbicide

Perspectivas futuras e impacto social de las biotecnologías vegetales

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