Transgenic Potato Cultivars Resistant to Potato Virus X

Hoekema, A.; Huisman, Marianne J.; Willink, Dinie Posthumus-Lutke; Jongedijk, Erik; Elzen, Peter van den; Cornelissen, Ben J. C.

doi:10.1007/978-1-4615-3304-7_19

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…The transformation of potato cv. ‘Désirée’ was performed using routine transformation protocols ( Fillatti et al., 1987 , Hoekema et al., 1991 ). Among 35 independent primary transformants, the resistant event A31-47 was selected after growth under greenhouse conditions (18–22 °C, 16 h of light and 8 h of dark) and field condition.…”

Section: Methodsmentioning

confidence: 99%

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Aguilera-Gálvez

Champouret

Rietman

et al. 2018

Studies in Mycology

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Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.

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Section: Methodsmentioning

confidence: 99%

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Aguilera-Gálvez

Champouret

Rietman

et al. 2018

Studies in Mycology

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Genetically engineered resistance to potato virus X in four commercial potato cultivars

Khalilian

Eweida

et al. 1995

Plant Cell Reports

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The genes for the capsid protein (CP) and the 8K movement protein of PVX were introduced into potato (Solanum tuberosum L.) and expressed under the control of CaMV 35S promoter using a binary vector andAgrobacterium tumefaciens. Four commercial potato cultivars (Russet Burbank, Shepody, Desirée and Bintje) have been efficiently transformed. Eleven independent transgenic clones, with CP expression levels higher than 0.05% of the soluble leaf proteins, were analyzed for resistance to inoculation with PVX (5 and 50µg/ml). The resistance of the transgenic plants to PVX was observed with the lower titer of virus inoculation (5 µg/ml) but not with higher titer (50 µg/ml). A significant reduction in the accumulation of virus in the inoculated transgenic potato plants has been observed under greenhouse and field conditions. Furthermore, the CP gene is very stable and is transferred to new plants originated from stem cuttings or from tubers. The transgenic plants appeared to be phenotypically identical to the nontransformed controls.

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Transgenic Potato Cultivars Resistant to Potato Virus X

Cited by 2 publications

References 27 publications

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

Genetically engineered resistance to potato virus X in four commercial potato cultivars

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