Transgenic plants of Lolium multiflorum , Lolium perenne , Festuca arundinacea and Agrostis stolonifera by silicon carbide fibre-mediated transformation of cell suspension cultures

Genetic Engineering of Plants for Resistance to Viruses

“…Success has however been reported with maize (Bullock et al, 2001;Kaepler et al, 1992;Petolino et al, 2000;Wang et al, 1995), rice (Nagatani, 1997), wheat (Brisibe, et al, 2000;Serik, et al, 1996), tobacco (Kaeppler et al, 1990), Lolium multiflorum, L. perenne, Festuca arundinacea, and Agrostis stolonifera (Dalton et al, 1998).…”

Whisker-mediated transformation of peanut with chitinase gene enhances resistance to leaf spot disease

“…This means that whiskers puncture the cell only during vortexing, opening the way for DNA entry. However, to develop a protocol for consistent transgenic plant production, some marker genes were used (Dalton et al 1998). In this sense, the conditions for a successful whisker-mediated DNA transformation have been studied using the transient expression of reporter genes (Nagatani et al 1997, Iqbal et al 2012.…”

Genotypic variation of Agrobacterium-mediated transformation of Italian ryegrass

“…The susceptibility of Italian ryegrass to gene manipulation has been well established by direct gene transfer methods such as particle bombardment (Ye et al 1997;Dalton et al 1999;Takahashi et al 2002) or by silicon-carbide fibermediated transformation (Dalton et al 1998). For the first time, Ye et al (1997) generated transgenic Italian ryegrass using embryogenic suspension cells as target for particle bombardment-mediated transformation system.…”

“…Although these studies generated higher number of transgenic Italian ryegrass; unfortunately, cultivars from which transgenic plants were regenerated were not mentioned. Dalton et al (1998) also produced transgenic Italian ryegrass using silicon-carbide-fibermediated transformation system which is considered as simpler and less expensive comparative to microprojectile bombardment, however; in terms of efficiency of resources the microprojectile bombardment method is preferable since larger volume of cells can be treated at one time (Dalton et al 1998;Dalton et al 1999). Although direct gene transfer technology has been used successfully to produce transgenic Italian ryegrass, it has been claimed in several studies that transgenic plants obtained by direct gene transfer methods through protoplast or particle bombardment showed multiple copy insertions of the transgenes (Dalton et al 1995;Spangenberg et al 1995).…”