Transgenic Plants as Biofactories for the Production of Biopharmaceuticals: A Case Study of Human Placental Lactogen

Urreta, Iratxe; Castañón, Sonia

doi:10.5772/31515

Cited by 3 publications

(1 citation statement)

References 113 publications

(115 reference statements)

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“…Analysis of the deleterious effects of recombinant protein accumulation on plant physiology and growth is crucial in order to assess the economic feasibility of using transgenic plants as biofactories, and this is largely dependent on the target protein function 65 . In our scenario with BvLz m , we found no deleterious effects of enhanced BvLz m expression on sugarcane growth.…”

Section: Resultsmentioning

confidence: 99%

Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system

Damaj¹,

Jifon

Woodard

et al. 2020

Sci Rep

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Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, highbiomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter:BvLz lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter:BvLz lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter:BvLz lines with additional BvLz expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing highvalue proteins and bioproducts. Recombinant proteins are currently being produced in cultured cell-based systems in mammals, microbes (bacteria and yeast), insects and plants, as well as in transgenic animals (reviewed by Demain and Vaishnav) 1. Transgenic plants constitute an attractive system for expression and production of a variety of proteins and biomolecules due to their efficient eukaryotic protein synthesis, high scalability, relatively low production costs and environmental footprint 2-4. However, selecting suitable hosts and expression vectors are key considerations since protein accumulation is determined by expression levels. Important factors to consider when selecting a plant-based production platform include biomass yield per hectare, recombinant protein yield per unit biomass, ease of transformation, scalability and safety 5. Sugarcane

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Section: Resultsmentioning

confidence: 99%

Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system

Damaj¹,

Jifon

Woodard

et al. 2020

Sci Rep

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A Review on Edible Vaccines and Biopharmaceutical Products from Plants

Paradia¹,

Bhavale

Agnihotri

et al. 2023

CPB

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Plants have substantial potential for the development of various biopharmaceuticals. Plants provide a cost-effective and direct source for the production of biopharmaceuticals such as vaccines, antibodies, proteins, enzymes and hormones. In most cases, purification is an important and expensive step in the production of these substances. The problem can be resolved when it is produced in plants and the whole plant can be consumed. Direct ingestion of plant materials may help in overcoming the purification step. Being produced in seeds, fruits and tubers, it helps in providing more immunization in developing countries at a cheaper rate. Moreover, it can be administered more efficiently than any other dosage forms. This review focuses on various immunization and therapeutic products that are produced in plants along with currently available formulations in each category.

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Plant Biotechnology for Industrial Production

Takayama¹,

Mera²,

Akita³

2012

J. Sci. High Tech. Agric.

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This paper presents an overview of the applications of plant biotechnology for industrialscale production of clonal plants, secondary metabolites and heterologous proteins. Clonal plants are produced at industrial-scale tissue culture nurseries with tissue culture facilities, and these production systems have been scaled-up recently with the use of bioreactors. Plant biotechnology-based systems for production of secondary metabolites and/or heterologous proteins have been optimized in plants through various expression strategies. Recently, the production of secondary metabolites in large-scale bioreactors has been commercialized. Furthermore, heterologous proteins from various eukaryotic origins such as antigens, as well as those with vaccine value are expressed in plants. Studies on the production of a number of commercially interesting products currently being investigated are described.

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Transgenic Plants as Biofactories for the Production of Biopharmaceuticals: A Case Study of Human Placental Lactogen

Cited by 3 publications

References 113 publications

Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system

Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system

A Review on Edible Vaccines and Biopharmaceutical Products from Plants

Plant Biotechnology for Industrial Production

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