Transgenic Pearl Millet (Pennisetum glaucum)

Lambé, Pascal; Dinant, Monique; Deltour, R.

doi:10.1007/978-3-642-59612-4_6

Cited by 17 publications

(8 citation statements)

References 39 publications

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“…Pearl millet callus was transformed and confirmed by Southern blot analysis for integration of the hygromycin resistance gene although plants were not recovered (Lambe et al 1995). A limited number of T 0 transgenic pearl millet plants containing the hygromycin resistance gene were recovered and confirmed by Southern blot analysis (Lambe et al 2000); however, molecular confirmation of transgene transmission to progeny was not shown and apparently only three plants produced progeny that segregated for hygromycin resistance. Lambe et al (2000) also compared hygromycin and phosphinothricin (PPT) selection but were not able to recover PPT-resistant plants.…”

Section: Introductionmentioning

confidence: 99%

Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues

Goldman¹,

Hanna²,

Fleming³

et al. 2003

Plant Cell Reports

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Pearl millet [ Pennisetum glaucum (L.) R. Br.] is a drought-tolerant cereal crop used for grain and forage. Novel traits from outside of the gene pool could be introduced provided a reliable gene-transfer method were available. We have obtained herbicide-resistant transgenic pearl millet plants by microprojectile bombardment of embryogenic tissues with the bar gene. Embryogenic tissues derived from immature embryos, inflorescences and apical meristems from diploid and tetraploid pearl millet genotypes were used as target tissues. Transformed cells were selected in the dark on Murashige and Skoog medium supplemented with 2 mg/l 2,4-D and 15 mg/l phosphinothricin (PPT). After 3-10 weeks in the dark, herbicide-resistant somatic embryos were induced to germinate on MS medium containing 0.1 mg/l thidiazuron and 0.1 mg/l 6-benzylaminopurine. Plants were transferred to the greenhouse after they were rooted in the presence of PPT and had passed a chlorophenol red assay (the medium turned from red to yellow). Transgenic plants were recovered from bombardments using intact pAHC25 plasmid DNA, a gel-purified bar fragment, or a mixture of pAHC25 plasmid or bar fragment and a plasmid containing the enhanced green fluorescent protein ( gfp) gene (p524EGFP.1). Analyses by the polymerase chain reaction, Southern blot hybridization, GFP expression, resistance to herbicide application, and segregation of the bar and gfp genes confirmed the presence and stable integration of the foreign DNA. Transformed plants were recovered from all three explants, although transformation conditions were optimized using only the tetraploid inflorescence. Time from culture initiation to rooted transgenic plant using the tetraploid inflorescence ranged from 3-4 months. Seven independent DNA/gold precipitations were used to bombard 52 plates, 29 of which produced an average of 5.5 herbicide-resistant plants per plate. The number of herbicide-resistant plants recovered per successful bombardment ranged from one to 28 and the frequency of co-transformation with gfp ranged from 5% to 85%.

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Section: Introductionmentioning

confidence: 99%

Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues

Goldman¹,

Hanna²,

Fleming³

et al. 2003

Plant Cell Reports

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“…Presence of co-transformed gus gene was observed on Southern hybridization in 60% of the hygromycin resistant clones by Lambé et al (2000). 82% of all the analyzed transformants contained the intact hph gene expression cassette and all of them had the intact gus gene expression unit.…”

Section: Selection and Plant Regenerationmentioning

confidence: 96%

“…Lambé et al (1995Lambé et al ( , 2000 Vasil and Vasil 1981a, b;Vasil 1983, 1984;Taylor andVasil 1995, 1996 Xu et al 1984;Rao et al 1988;Reddy et al 1988Reddy et al , 1990Kavi Kishor et al 1992;Zeatin, NAA Osuna-Avilla et al 1995;Vishnoi and Kothari 1996 Abbreviations: 2,4-D, 2,4-dichlorophenoxy acetic acid; 2,4,5-T, 2,4,5-trichlorophenoxy acetic acid; NAA, a-naphthalene acetic acid; BAP, 6-benzylamino purine; Kn, Kinetin; IAA, Indole-3-acetic acid; pCPA, para chlorophenoxy acetic acid; Pic, 4-amino-3,5-6-trichloropicolinic acid; TDZ, Thidiazuron; IBA, Indole-3-butyric acid; GA 3 , Gibberellic acid.…”

Section: Vectorsmentioning

confidence: 99%

“…In a later study on Pearl millet, Lambé et al (2000) reported regeneration of hygromycin resistant plants obtained from type II callus.…”

Section: Selection and Plant Regenerationmentioning

confidence: 99%

“…But, production of transgenic plants in millets remain restricted only to pearl millet (Lambé et al 2000;Girgi et al 2002) and bahia grass (Smith et al 2002). Other small millets have been overlooked due to economic or regional considerations.…”

Section: Future Prospectsmentioning

confidence: 99%

See 2 more Smart Citations

Applications of biotechnology for improvement of millet crops: Review of progress and future prospects

Kothari

Kumar

Vishnoi

et al. 2005

Plant Biotechnology

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Pearl Millet

O’Kennedy

Crampton

Ozias‐Akins

2008

Compendium of Transgenic Crop Plants

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Pearl millet ( Pennisetum glaucum (L.) R. Br.) is a staple food that supplies a major proportion of calories and protein to large segments of the populations living in the semi‐arid tropical regions of Africa and Asia. The crop is supremely adapted to heat and aridity, and production is likely to increase as the world gets hotter and drier. The grain is nutritious, and has higher protein and energy levels than maize or sorghum. Pearl millet has an abundant natural diversity with approximately 140 species or subspecies belonging to the genus Pennisetum . Detailed genetic maps of pearl millet of some 300 loci spread over 7 linkage groups are available and 3437 pearl millet sequences have been deposited in the National Centre for Biotechnology Information database. Breeding objectives for pearl millet include tolerance to drought, enhanced nutritional quality, adaptation to saline conditions, stover feeding value, high tillering, and resistance to major pearl millet diseases such as downy mildew, ergot, smut, rust, and head mold of pearl millet. A new green revolution will necessitate the application of recent advances in plant breeding, including new tissue culture techniques, marker‐aided selection, mutagenesis, and genetic modification to meet our increasing requirement for food, feed, fodder, and fuel, with cereal grains playing a pivotal role.

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Transgenic Pearl Millet (Pennisetum glaucum)

Cited by 17 publications

References 39 publications

Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues

Fertile transgenic pearl millet [Pennisetum glaucum (L.) R. Br.] plants recovered through microprojectile bombardment and phosphinothricin selection of apical meristem-, inflorescence-, and immature embryo-derived embryogenic tissues

Applications of biotechnology for improvement of millet crops: Review of progress and future prospects

Pearl Millet

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