Transgenic microalgae as green cell-factories

Léon, Rosa; González-Ballester, David; Galván, Aurora; Fernández, Emilio

doi:10.1016/j.tibtech.2003.11.003

Cited by 246 publications

(112 citation statements)

References 61 publications

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“…Impediments to large-scale culture of microalgae are mainly economic and are tied to the investment requirements for the algae cultivation. One solution would be to increase the oil productivity by genetic and metabolic engineering (León-Bañares et al, 2004;Mathews & Wang, 2009). One may expect the expansion of algal technology via CO 2 filtration because power plants can incorporate this technology immediately into their management systems.…”

Section: Management and Sustainabilitymentioning

confidence: 99%

The Challenge of Bioenergies: An Overview

Carels¹

2011

Biofuel's Engineering Process Technology

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Section: Management and Sustainabilitymentioning

confidence: 99%

The Challenge of Bioenergies: An Overview

Carels¹

2011

Biofuel's Engineering Process Technology

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“…Several transcriptomic analyses have also been conducted with Nannochloropsis (Vieler et al, 2012;Carpinelli et al, 2013;Zheng et al, 2013); however, the individual-snapshot mRNA analyses, which tested only one or two time points, has failed to capture the temporal dynamics of gene expression. Moreover, the paucity of studies correlating gene expression and metabolite data during oleaginousness has precluded inference and untangling of the causal relationships underlying the complex molecular network involved in lipid metabolism (León-Bañares et al, 2004;Rosenberg et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Choreography of Transcriptomes and Lipidomes ofNannochloropsisReveals the Mechanisms of Oil Synthesis in Microalgae

et al. 2014

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To reveal the molecular mechanisms of oleaginousness in microalgae, transcriptomic and lipidomic dynamics of the oleaginous microalga Nannochloropsis oceanica IMET1 under nitrogen-replete (N+) and N-depleted (N-) conditions were simultaneously tracked. At the transcript level, enhanced triacylglycerol (TAG) synthesis under N-conditions primarily involved upregulation of seven putative diacylglycerol acyltransferase (DGAT) genes and downregulation of six other DGAT genes, with a simultaneous elevation of the other Kennedy pathway genes. Under N-conditions, despite downregulation of most de novo fatty acid synthesis genes, the pathways that shunt carbon precursors from protein and carbohydrate metabolic pathways into glycerolipid synthesis were stimulated at the transcript level. In particular, the genes involved in supplying carbon precursors and energy for de novo fatty acid synthesis, including those encoding components of the pyruvate dehydrogenase complex (PDHC), glycolysis, and PDHC bypass, and suites of specific transporters, were substantially upregulated under N-conditions, resulting in increased overall TAG production. Moreover, genes involved in the citric acid cycle and b-oxidation in mitochondria were greatly enhanced to utilize the carbon skeletons derived from membrane lipids and proteins to produce additional TAG or its precursors. This temporal and spatial regulation model of oil accumulation in microalgae provides a basis for improving our understanding of TAG synthesis in microalgae and will also enable more rational genetic engineering of TAG production.

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“…These strategies may result in productivity gains, enabling commercialscale production. Such strategies include codon optimization (León-Bañares et al 2004), development of alternative vectors Zschoernig 2007, Lauersen et al 2013) and modifications to the design expressed constructs. Changes in constructs include the addition of autocleavage peptide (Rasala et al 2012), use of fused promoters (EichlerStahlberg et al 2009, Schroda et al 2000 and addition of intronic sequences (EichlerStahlberg et al 2009, Lumbreras et al 1998.…”

Section: Expression Systemmentioning

confidence: 99%