Transgenic Insertional Mutagenesis: Applications to Inner-Ear Genetics

Friedman, Rick A.

doi:10.1001/archotol.1996.01890150030006

Cited by 3 publications

(2 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The aim is to inactivate the gene by inserting a piece of foreign DNA into its normal sequence. Gene trapping, that is untargeted insertional mutation based upon random incorporation of DNA sequences throughout the genome, is useful for locating previously unidentified genes [10,11], Poten tially useful mutants arc initially identified by behavioural defects such as loss of hearing or balance. Several important genes have been identified this way [6,[12][13][14], and many more arc likely to follow.…”

Section: Transgenic Micementioning

confidence: 99%

Production of Conditionally Immortalised Cell Lines from a Transgenic Mouse

Holley

Lawlor²

1997

Audiol Neurotol

View full text Add to dashboard Cite

This review describes the H2k^btsA58 transgenic mouse (Immortomouse) and its application to the production of conditionally immortalised cell lines from sensory epithelia within the mammalian inner ear. Established cell lines should overcome many of the technical difficulties associated with experimental procedures in auditory and vestibular research. These include the limited amount of tissue available and the relatively complex and laborious dissection. Conditional immortalisation should also allow essential studies on the molecular and cellular mechanisms that govern both the differentiation of sensory cells and the development of sensory epithelia.

show abstract

Section: Transgenic Micementioning

confidence: 99%

Production of Conditionally Immortalised Cell Lines from a Transgenic Mouse

Holley

Lawlor²

1997

Audiol Neurotol

View full text Add to dashboard Cite

show abstract

“…The avail ability of mouse embryonic stem cells capable of reconstituting the animal means that ma nipulation of the mouse genome is relatively commonplace, and investigation of gene func tion by the use of'knockout' strategies to gen erate null alleles [4] has demonstrated roles in the development of the inner ear fora number of genes. The generation of transgenic mice by the introduction of exogenous DNA into the genome can on occasions (5-10%) lead to dis ruption of an endogenous gene with concur rent phenotypic consequences [ 13], including effects on inner ear development and function [14].…”

Section: Introductionmentioning

confidence: 99%

Paradigms and Paradoxes: Mouse (and Human) Models of Genetic Deafness

Hughes

1997

Audiol Neurotol

View full text Add to dashboard Cite

Mouse models have proved valuable tools in the analysis of human genetic disorders. The identification of the genes mutated in classical mouse mutants and the analysis of the phenotype of mutants following targeted gene disruption have provided some clarification of the development and functioning of the inner ear. A number of these genes also play a role in human deafness. Analysis of mutations in both human and mouse deafness genes has identified a number of distinct phenomena that contribute to the observed phenotype.

show abstract