Transgenic crops for improved pharmaceutical products

Oksman-Caldentey, Kirsi-Marja; Hiltunen, R.

doi:10.1016/0378-4290(95)00059-3

Cited by 57 publications

(30 citation statements)

References 56 publications

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“…Among T-DNA genes, the rol oncogenes cause striking phenotypical and biochemical alterations in transformed plants (Casanova et al, 2005;Christey, 2001;Oksman-Caldentey and Hiltunen, 1996;Piispanen et al, 2003). Moreover, three rol genes, rolA, rolB, and rolC or its combination (rolABC), are sufficient to induce hairy roots in Nicotiana tabacum (Cardarelli et al, 1987;Spena et al, 1987), Daucus carota (Capone et al, 1989), Ginkgo biloba (Ayadi and Trémouillaux-Guiller, 2003) and Catharanthus roseus (Hong et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli

Shkryl

Veremeichik

Bulgakov

et al. 2007

Biotech & Bioengineering

161

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It is known that the rolA, rolB, and rolC genes of Agrobacterium rhizogenes T-DNA affect processes of plant development and activate the synthesis of secondary metabolites in transformed plant cells. Although a synergistic activity of the rol genes on root formation is well-documented, little is known about their individual and combined action on secondary metabolism. In the present investigation, we provide evidence indicating that individual rolA, rolB, and rolC genes are capable of increasing biosynthesis of anthraquinones (AQs) in transformed calli of Rubia cordifolia. The stimulatory effect was due to the increased transcription of a key gene of AQ biosynthesis, the isochorismate synthase (ICS) gene. The strongest AQ-stimulating activity was shown for an R. cordifolia culture expressing rolB at high levels, where rolB ensured a 15-fold increase of AQ accumulation compared with the control, non-transformed calli. A tyrosine phosphatase inhibitor abolished the rolB-induced increase of AQ production, thus indicating the involvement of tyrosine (de)phosphorylation in the rolB-mediated AQ stimulation. The rolA- and rolC-expressing cultures produced 2.8- and 4.3-fold higher levels of AQs, respectively, when compared with the control calli. However, the effect of rolA, rolB, and rolC on AQ biosynthesis was not synergistic because rolA and rolC apparently attenuated the stimulatory effect of rolB on AQ biosynthesis. Therefore, the rol-gene-mediated signals that promote root formation and those which activate biosynthesis of secondary metabolites seem to have a point of divergence.

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Section: Introductionmentioning

confidence: 99%

Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli

Shkryl

Veremeichik

Bulgakov

et al. 2007

Biotech & Bioengineering

161

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“…However, differentiated tissues display both genetic and biochemical stability (Flores & Curtis, 1992). For this reason, genetically transformed roots are particularly interesting due to their capacity for long-term growth (Flores et al, 1999;Momčilović et al, 2001;Zdravković-Korać et al, 2003), and therefore widely used as a source of many useful compounds (Flores & Curtis, 1992;Oksman-Caldentey & Hiltunen, 1996;Flores et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Determination of escin content in androgenic embryos and hairy root culture ofAesculus hippocastanum

Ćalić¹,

Zdravković-Korać²,

Šavikin-Fodulović³

et al. 2010

Pharmaceutical Biology

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“…Furthermore, hairy roots have been found to synthesize secondary metabolites at similar or higher levels to those found in whole plants (Flores 1987;Hu and Du 2006;Oksman-Caldentey and Hiltunen 1996;Rhodes et al 1994;Sevón and Oksman-Caldentey 2002) and to have light-guiding properties (Towler et al 2006). In addition to their recombinant protein expression ability, transgenic hairy root cultures exhibit a significantly greater long-time stability than transgenic suspension cultures (Sharp and Doran 2001).…”

Section: Hairy Root Culturesmentioning

confidence: 95%

Plant Cell-Based Bioprocessing

Eibl

2009

Cell and Tissue Reaction Engineering

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Plant cell-based bioprocessing is the use of plant cell and tissue cultures for the production of biologically active substances (low molecular secondary metabolites and recombinant proteins). The most significant advantage of plant cell culture over the traditionally grown whole wild plant or engineered transgenic plant is the sterile production of metabolites under defined controlled conditions independent of climatic changes and soil conditions, which means that variations in product yield and quality can be better avoided. Furthermore, regulatory requirements such as the cGMP standards, which have to be adhered to in the early stages of pharmaceutical production, are more easily met.Moreover, plant cells are capable of performing complex posttranslational processing, which is a precondition for heterologous protein expression. When compared with mammalian cells, which currently dominate in the commercial protein manufacture, plant cell cultures as alternative expression systems guarantee safer processes because there is a lower risk of contamination by mammalian viruses, pathogens, and toxins. In addition to this considerable advantage, the process costs can also be substantially reduced. This is due to the fact that plant cell culture medium is very simple in composition and therefore relatively inexpensive.This chapter provides an overview of culture types, techniques, and suitable bioreactors used to produce secondary metabolites and recombinant proteins in plant cells. We describe plant cell culture basics, discuss key topics relevant to plant cell bioreactor engineering with application examples, and give an overview of approaches to improving productivity of plant cell-based processes.

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Transgenic crops for improved pharmaceutical products

Cited by 57 publications

References 56 publications

Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli

Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli

Determination of escin content in androgenic embryos and hairy root culture ofAesculus hippocastanum

Plant Cell-Based Bioprocessing

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