Transgenic creeping bentgrass overexpressing <i>Osa‐miR393a</i> exhibits altered plant development and improved multiple stress tolerance

Zhao, Junlong; Yuan, Shuangrong; Zhou, Man; Yuan, Nannan; Li, Zhigang; Hu, Qian; Bethea, Frank; Liu, Haibo; Li, Shigui; Luo, Hong

doi:10.1111/pbi.12960

Cited by 83 publications

(56 citation statements)

References 111 publications

(161 reference statements)

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“…Our data indicated miR319 kept conserved functions in switchgrass as in other plant species in improving leaf width and thickness, suppressing tiller numbers, and enlarging stem diameters [25,26]. In addition, miR-NAs also showed species-specific functions, such as the opposite effect of miR393 in regulating tiller number among different plant species [20,38,39]. In this work, we observed miR319 played a positive role in improving stem elongation.…”

Section: Discussionsupporting

confidence: 53%

Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

Liu

Yan

Wang

et al. 2020

Biotechnol Biofuels

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Background: Switchgrass (Panicum virgatum L.), a C 4 perennial grass, has been recognized as one of the most potentially important lignocellulose biofuel crops. MicroRNA319 (miR319) plays a key role in plant development, abiotic resistance, and cell wall biosynthesis by repressing expression of its target TCP genes. We hypothesized miR319-TCP pathway could play important roles in switchgrass feedstock characteristics for biofuel production, and produced switchgrass transgenic plants overexpressing miR319 (by ectopic expressing Osa-MIR319b gene), blocking miR319 (by overexpressing a target mimicry of miR319/MIM319) and repression of miR319 target gene PvPCF5. Plant phenotype, biomass yield, and feedstock quality of transgenic plants were analyzed.Results: Overexpression of miR319 in switchgrass promoted leaf elongation and expansion of transgenic plants, increased plant height, stem diameter, and resulted in a significant increase in plant biomass yield. Transgenic plants overexpressing of miR319 reduced lignin content, showed significantly higher enzymatic hydrolysis efficiency compared to the wild type plant. However, opposite results were observed in the MIM319 plants. Furthermore, suppression of miR319 target gene PvPCF5 activity also reduced lignin content, increased lignin monomer S/G ratio and the proportion of β-O-4 linkages, while significantly improving the sugar production per plant. Quantitative real-time (qRT-PCR) analysis indicated that expression of PvMYB58/63B and PvHCT with predicted TCP binding sites in their promoter regions was negatively regulated by miR319-PvPCF5 module. Conclusions:MiR319-PvPCF5 module plays positive roles in regulating biomass yield and quality of switchgrass. It can be utilized as a candidate molecular tool in regulating biomass yield and feedstock quality. The finding could also be transferred to other grasses for forage quality improvement through genetic manipulation.

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Section: Discussionsupporting

confidence: 53%

Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

Liu

Yan

Wang

et al. 2020

Biotechnol Biofuels

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“…In switchgrass, transgenic plants overexpressing miR393 also showed more tillers and higher biomass yield in greenhouse and eld tests [17]. However, Osa-miR393a overexpressing creeping bentgrass had fewer and longer tillers compared with WT [31]. Wang et al [32] reported that overexpressing Osa-miR529 in rice inhibited SPL14/17 and enhanced the tiller number.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide Small Rna Profiling Reveals Tiller Development in Tall Fescue (Festuca Arundinacea Schreb.)

Wang

et al. 2020

Preprint

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Background: Tall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production (‘Ch-3’, high tiller production rate and ‘Ch-5’, low tiller production rate) andtwo types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). ‘Ch-3’, ‘Ch-5’, Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing.Results: A total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased abundance in both Ch-3 and Tillering plants compared with that in Ch-5 and Pre-tillering plants and were positive with tillering, while another four miRNAs were negative with tiller development. Out ofthe three miRNAs osa-miR156a, zma-miR528a-3p and osa-miR444b.2, the rest of 15 miRNAs were newfound controllers mediating tiller development in plants. Based on our previous full-length transcriptome analysis in tall fescue, 2 8927 potential target genes were discoveredfor all identified miRNAs. Most of the 212 target genes of the 18 miRNAs were dominantly enriched into “ubiquitin-mediated proteolysis”, “phagosome”, “fatty acid biosynthesis”, “oxidative phosphorylation”, and “biosynthesis of unsaturated fatty acids” KEGG pathways.Conclusions: This is the first genome-wide miRNA profiles analysis to identifyregulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass.

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“…Agrostis stolonifera Altered phenotype (fewer amount but longer tillers, and reduced stomata density and denser cuticles) and improved tolerance to multiple stresses (drought, heat and salinity, and increased uptake of potassium) Zhao et al (2019) Glycine max…”

Section: Osa-mir393amentioning

confidence: 99%

MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants

Basso

Ferreira

Kobayashi

et al. 2019

Plant Biotechnology Journal

107

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Summary Micro RNA s (mi RNA s) modulate the abundance and spatial–temporal accumulation of target mRNA s and indirectly regulate several plant processes. Transcriptional regulation of the genes encoding mi RNA s ( MIR genes) can be activated by numerous transcription factors, which themselves are regulated by other mi RNA s. Fine‐tuning of MIR genes or mi RNA s is a powerful biotechnological strategy to improve tolerance to abiotic or biotic stresses in crops of economic importance. Current approaches for mi RNA fine‐tuning are based on the down‐ or up‐regulation of MIR gene transcription and the use of genetic engineering tools to manipulate the final concentration of these mi RNA s in the cytoplasm. Transgenesis, cisgenesis, intragenesis, artificial MIR genes, endogenous and artificial target mimicry, MIR genes editing using Meganucleases, ZNF proteins, TALEN s and CRISPR /Cas9 or CRISPR /Cpf1, CRISPR / dC as9 or dC pf1, CRISPR 13a, topical delivery of mi RNA s and epigenetic memory have been successfully explored to MIR gene or mi RNA modulation and improve agronomic traits in several model or crop plants. However, advantages and drawbacks of each of these new biotechnological tools ( NBT s) are still not well understood. In this review, we provide a brief overview of the biogenesis and role of mi RNA s in response to abiotic or biotic stresses, we present critically the main NBT s used for the manipulation of MIR genes and mi RNA s, we show current efforts and findings with the MIR genes and mi RNA s modulation in plants, and we summarize the advantages and drawbacks of these NBT s and provide some alternatives to overcome. Finally, challenges and future perspectives to mi RNA modulating in important crops are also discussed.

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Transgenic creeping bentgrass overexpressing Osa‐miR393a exhibits altered plant development and improved multiple stress tolerance

Cited by 83 publications

References 111 publications

Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

Heteroexpression of Osa-miR319b improved switchgrass biomass yield and feedstock quality by repression of PvPCF5

Genome-wide Small Rna Profiling Reveals Tiller Development in Tall Fescue (Festuca Arundinacea Schreb.)

MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants

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