Transgenic Coffee (Coffea Species)

Spiral, J.; Leroy, Thierry; Paillard, Michel; Pétiard, Vincent

doi:10.1007/978-3-642-59609-4_5

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…2A), while the controls (non-transformed calli) did not show any uidA expression. Differently to previous reports (van Boxtel et al, 1995;Spiral et al, 1999;Hatanaka et al, 1999), endogenous GUS activity was not observed on the assayed coffee somatic embryogenic tissues. The use of methanol in the GUS assay may have helped to avoid eventual endogenous GUS activity (Kosugi et al, 1990).…”

Section: Resultscontrasting

confidence: 99%

“…The transgenic coffee plants presented normal phenotype during the vegetative phase and produced viable seeds (data not shown). This is in agreement with previous work, in which C. canephora transformed with A. tumefaciens presented normal phenotype while plants transformed with A. rhizogenes showed several different undesirable traits, such as short internodes and crinkled leaves (Spiral et al, 1999). Expression of the bar gene was verified by localized application of herbicide on in vivo acclimated plants.…”

Section: Resultssupporting

confidence: 92%

“…There are few works reporting regeneration of transgenic coffee plants (Hatanaka et al, 1999;Spiral et al, 1999;Leroy et al, 2000). Spiral et al (1993) reported difficulties in screening transformation events in coffee with the kanamycin selection system.…”

Section: Resultsmentioning

confidence: 99%

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Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

Ribas

Kobayashi

Pereira

et al. 2006

Braz. arch. biol. technol.

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 92%

See 1 more Smart Citation

Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

Ribas

Kobayashi

Pereira

et al. 2006

Braz. arch. biol. technol.

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“…Furthermore, a system for genic transfer is required that is simple, cheap, and reproducible. In coffee, there are efficient regeneration systems through somatic embryogenesis from several tissues and the genetic transformation of coffee plants has been achieved successfully by several research groups (Barton et al, 1991;Spiral et al, 1993;Sugiyama et al, 1995;van Boxtel et al, 1995;Hatanaka et al, 1999;Spiral et al, 1999;Leroy et al, 2000;Fernández-Da Silva and Menéndez-Yuffá, 2003;Ogita et al, 2004).…”

Section: Transformation Of Coffeementioning

confidence: 99%

Coffee biotechnology

Santos-Briones

Hernández‐Sotomayor

2006

Braz. J. Plant Physiol.

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In the last three decades, interest has turned to in vitro cell culture in different areas of coffee research. In vitro techniques have been applied not only for coffee improvement through genetic transformation but also to study various aspects in coffee cells such as chemical (caffeine synthesis and the production of coffee aroma), physiological and more recently, biochemical aspects. The most important advances obtained to date on in vitro coffee techniques in fields like biochemistry, physiology, regeneration systems and genetic engineering, are presented and discussed.

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“…Transgenic plants of C. canephora and C. arabica were obtained using genetic transformation mediated by A. rhizogenes (Spiral et al 1993;Sugiyama et al (1995). Transient expression of uid A gene was demonstrated by GUS histochemical assay on callus tissues derived from transformed protoplasts (Spiral et al 1999).…”

Section: Genetic Transformation Of Coffea Spmentioning

confidence: 99%

Developments in coffee biotechnology—in vitro plant propagation and crop improvement

Kumar

Naidu

Ravishankar

2006

Plant Cell Tiss Organ Cult

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Coffee is an important plantation crop grown in about 80 countries across the globe. In recent years, coffee attained lot of attention in the biotechnology research area. Since last three decades, there has been a steady flow of information on coffee biotechnology and now it is entering into the genomic era. Major milestones in coffee biotech research are successful in vitro manipulation and multiplication of coffee, development of gene transfer protocols and generation of transgenic coffee plants with specific traits. The isolation of genes involved in caffeine biosynthetic pathway has opened up new avenues for generating caffeine free transgenic coffee. With the initiation of international coffee genomics initiatives, the genomic research in coffee is expected to reach new dimensions. The IPR issues may play crucial role in sharing of benefits during international collaborations in near future. This review focuses on the basic and applied aspects of coffee biotechnology for newer potentials.

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Transgenic Coffee (Coffea Species)

Cited by 12 publications

References 24 publications

Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

Production of herbicide-resistant coffee plants (Coffea canephora P.) via Agrobacterium tumefaciens-mediated transformation

Coffee biotechnology

Developments in coffee biotechnology—in vitro plant propagation and crop improvement

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