Transgenerational effects of developmental exposure to chlorpyrifos-oxon in zebrafish (DANIO RERIO)

Epigenetic Transgenerational Modifications Induced by Xenobiotic Exposure in Zebrafish

“…The levels of 5-methylcytosine (5-mC) can be measured in a simplified "one-step" ELISA type reaction; however this footprint may be biased since not all xenobiotics produce the same effect on methylation levels, and each cell type may express a different signature throughout development and different life stages (Santangeli et al, 2019;Pierron et al, 2021). For instance, no differences were observed in F0 and subsequent generations after bisphenol-A (BPA) and arsenic (As) exposure (Santangeli et al, 2019;Valles et al, 2020), while different levels of DNA methylation were observed in different generations after exposure to chlorpyrifos (F1 and F2 showed hypomethylation, whereas F3 presented hypermethylation) probably due to a paternal and/or maternal influence (Schmitt et al, 2020). Thus, global DNA methylation may not be the most sensitive marker to assess xenobiotic-induced transgenerational effects in zebrafish.…”

Epigenetics in neurodegenerative disorders induced by pesticides

“…In addition, the adverse effects of developmental exposure to the active CPO have been proved to persist into adulthood even future generations [79]. Schmitt et al demonstrated that early life stage exposures to CPO can lead to epigenetic changes in neurological activity, which may lead to alterations in response to CPO in future generations [34]. Liu' team also investigated H3K4me3 and DNA methylation levels of the PPARγ gene in the placenta was associated with prenatal chlorpyrifos exposure, and could effect on birth outcomes and neurodevelopment [80].…”