Transfusion transmitted virus infection in general populations and patients with various liver diseases in south China

Chen, Yong Peng

doi:10.3748/wjg.v6.i5.738

Cited by 9 publications

(3 citation statements)

References 16 publications

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“…However, many investigation data revealed that the patients infected with HGV or TTV were usually asymp tomatic and only a few of them showed mild liver injury [42][43][44] . Therefore, a wide variety of questions about the potential pathogenicity of HGV and TTV infection remain unanswered [31, [45][46][47] . Since HGV and TTV are generally transmitted by transfusion, high infection frequencies of the two viruses in blood recipients and in hemodialyzed patients have been demonstrated [31,43,[48][49][50] .…”

Section: Discussionmentioning

confidence: 99%

High frequencies of HGV and TTV infections in blood donors in Hangzhou

Yan¹,

Chen²,

Luo³

et al. 2001

WJG

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AIM To determine the frequencies of HGV and TTV infections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.RESULTS Thirty-two (15.8%) and 30 (14.8%) of the 203 serum samples were positive for HGV RNA and TTV DNA, respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA. Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3 serum samples compared with the reported HGV and TTV sequences was 89.36%, 87.94%, 88.65% and 63.51%, 65.77% and 67.12%, respectively.CONCLUSION The infection rates of HGV and/or TTV in blood donors are relatively high, and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas.Subject headings China; DNA virus infections/epidemiology; hepatitis, virus, human/ epidemiology; blood transfusion/ adverse effect; blood donors; hepatitis agents, GB/ isolation & purification

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Section: Discussionmentioning

confidence: 99%

High frequencies of HGV and TTV infections in blood donors in Hangzhou

Yan¹,

Chen²,

Luo³

et al. 2001

WJG

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“…The mechanisms of carcinogenesis may be related with the block of GJIC of homologous and heterologous communication between tumor cells and surrounding normal cells [26] . Connexin32 (Cx32) and connexin43 (Cx43) are the major kinds of gap junction proteins expressing in hepatocytes [18] . Our works showed that expressions of Cx32,Cx43 proteins decreased in hepatocellular carcinoma tissues and cell lines whereas increased in normal tissues and cell lines [19][20][21] , but cx32 mRNA and cx43 mRNA kept a high level in both hepatocellular carcinoma and normal liver cells lines described previously [22,23] .…”

Section: Introductionmentioning

confidence: 99%

“…Hepatocellular carcinoma has been one of the most common malignant threatening human health [1][2][3][4][5][6][7][8][9] , and much higher incidences of human hepatocellular carcinoma (HCC) are observed recently [10][11][12][13][14][15][16][17][18] . Gap junctional intercellular communication (GJIC) is performed by intercellular hemichannels [19] , which are formed by six basic protein subunits named connexin (Cx) expressed in neighboring cells [20] .…”

Section: Introductionmentioning

confidence: 99%

Signal transduction of gap junctional genes, connexin32, connexin43 in human hepatocarcinogenesis

Ma²,

Sui³

et al. 2003

WJG

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AIM:To inve s ti ga te ga p ju n ct io na l i nt er c el lu la r communication (GJIC) in hepatocellular carcinoma cell lines, and signal transduction mechanism of gap junction genes connexin32(cx32),connexin43(cx43) in human hepatocarcinogenesis. METHODS:Scarped loading and dye transfer (SLDT) was employed with Lucifer Yellow (LY) to detect GJIC function in hepatocellular carcinoma cell lines HHCC, SMMC-7721 and normal control liver cell line QZG. After Fluo-3AM loading, laser scanning confocal microscope (LSCM) was used to measure concentrations of intracellular calcium [Ca 2+ ]i in the cells. The phosphorylation on tyrosine of connexin proteins was examined by immunoblot. RESULTS:SLDT showed that ability of GJIC function was higher in QZG cell than that in HHCC and SMMC-7721 cell lines. By laser scanning confocal microscopy, concentrations of intracellular free calcium [Ca

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