Transforming growth factor-6 stimulates bone ongrowth: Hydroxyapatite-coated implants studied in dogs

Lind, Martin; Overgaard, Søren; Nguyen, Tue H.; Ongpipattanakul, Boonsri; Bünger, Cody; Søballe, Kjeld

doi:10.3109/17453679608997766

Cited by 86 publications

(62 citation statements)

References 21 publications

(20 reference statements)

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“…Previous studies using a similar implant model in the dog have reported varied results with regard to the effect of TGF-β1 on implant fixation. TGF-β1 adsorbed to a hydroxyapatite coating only increased bone ongrowth by one-third, while no improvement was seen in gap healing and mechanical fixation compared to the control implants (Lind et al 1996a) When TGF-β1 was adsorbed to a tricalcium phosphate coating, bone ongrowth and gap healing was increased by 50% (Lind et al 1996b).…”

Section: Discussionmentioning

confidence: 99%

Locally delivered TGF-β1 and IGF-1 enhance the fixation of titanium implants: A study in dogs

et al. 2006

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Background Osteogenic growth factors have been suggested to enhance the fixation of implants used in joint replacement. We examined the effect of locally delivered transforming growth factor-β1 and insulin-like growth factor-1 in a biodegradable poly (D, L-lactide) coating.Material and methods In a paired study using 9 dogs, unloaded titanium implants surrounded by a 1-mm gap were inserted into the proximal humerus. The growth factors were incorporated in a poly (D, L-lactide) coating at a 1% (w/w) ratio of TGF-β1 and a 5% (w/w) ratio of IGF-1. Control implants were uncoated. After 4 weeks, the implants were evaluated by mechanical pushout test and by histomorphometry.Results A twofold increase was seen in mechanical fixation (strength, stiffness, energy absorption) for the growth factor-treated implants (p = 0.04). Similar results were seen in histomorphometry, as bone ongrowth was 2.5 times higher (p = 0.02), and gap healing was 30-110% higher (p = 0.04) for the growth factor-treated implants than for the control implants. Ongrowth of fibrous tissue was eliminated by the treatment.Interpretation TGF-beta-1 and IGF-1, locally delivered in a biodegradable poly(D,L-lactide) coating, enhance the mechanical fixation and osseointegration of titanium implants in cancellous bone, and no fibrous tissue is produced in the growth factor treated implants.

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Section: Discussionmentioning

confidence: 99%

Locally delivered TGF-β1 and IGF-1 enhance the fixation of titanium implants: A study in dogs

et al. 2006

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“…Secondly, biochemical modifications of the surface can also be designed with the aim of controlling the biological reaction at the interface by means of the delivery of specific molecules. This includes, for example, immobilisation of proteins or peptides on the surface of the biomaterial or controlled release of osteogenic growth factors (Lind et al, 1996;Lind, 1998;Elmengaard et al, 2005;Oates et al, 2007) and the coating of implant surfaces with minerals, such as calcium phosphate (Hayakawa et al, 2002). It is clear as well that further progress within these techniques is still needed and this demands deeper knowledge of the biological cascade associated to early bone healing around the implant.…”

Section: Introductionmentioning

confidence: 99%

Bone ingrowth on the surface of endosseous implants. Part 1: Mathematical model

Moreo

García-Aznar

Doblaré

2009

Journal of Theoretical Biology

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“…Although some encouraging results have been reported [4][5][6], a major drawback with the adsorption method is that it provides little, if any, control over delivery, including retention and orientation, of molecules. Proteins are initially retained on the surface by weak physisorption forces, and then, depending on the implant microenvironment, which varies among anatomical sites and among patients, the molecules desorb from the surface in an uncontrolled manner to interact with cells.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Sharon

Puleo

2008

Acta Biomaterialia

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Attachment of growth factors to biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), may enhance and/or accelerate integration of tissue engineering scaffolds. Although proteins are commonly bound via abundant amino groups, a more selective approach may increase bioactivity of immobilized molecules. In this research, exposed carboxyl groups on acid-terminated PLGA were modified with dihydrazide spacer molecules. The number of hydrazide groups available for subsequent attachment of protein was dependent on dihydrazide length, with shorter molecules present at significantly greater surface densities. The potent angiogenic glycoprotein vascular endothelial growth factor (VEGF) was oxidized with periodate and the aldehyde moieties allowed to react with the hydrazide-derivatized PLGA. Derivatization initially affected the amount of protein bound to the surfaces, but differences were substantially reduced following overnight incubation in saline. More importantly, use of shorter dihydrazide spacers significantly enhanced accessibility of immobilized VEGF for binding neutralizing antibody and soluble VEGR receptor. Furthermore, immobilized growth factor enhanced endothelial cell proliferation, with surfaces having the shortest and longest spacers stimulating greater effects. The present work has not only demonstrated an alternative approach to immobilizing growth factors on biodegradable materials, but the scheme can be used to alter the amount of protein bound as well as its availability for subsequent biointeractions.

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Transforming growth factor-6 stimulates bone ongrowth: Hydroxyapatite-coated implants studied in dogs

Cited by 86 publications

References 21 publications

Locally delivered TGF-β1 and IGF-1 enhance the fixation of titanium implants: A study in dogs

Locally delivered TGF-β1 and IGF-1 enhance the fixation of titanium implants: A study in dogs

Bone ingrowth on the surface of endosseous implants. Part 1: Mathematical model

Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

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